Alfalfa(Medicago sativa)is one of the most important forage crops in the world;however,its molecular genetics and breeding research are hindered due to the lack of a high-quality reference genome.Here,we report a de n...Alfalfa(Medicago sativa)is one of the most important forage crops in the world;however,its molecular genetics and breeding research are hindered due to the lack of a high-quality reference genome.Here,we report a de novo assembled 816-Mb high-quality,chromosome-level haploid genome sequence for‘Zhongmu No.1’alfalfa,a heterozygous autotetraploid.The contig N50 is 3.92 Mb,and 49165 genes are annotated in the genome.The alfalfa genome is estimated to have diverged from M.truncatula approximately 8 million years ago.Genomic population analysis of 162 alfalfa accessions revealed high genetic diversity,weak population structure,and extensive gene flow from wild to cultivated alfalfa.Genome-wide association studies identified many candidate genes associated with important agronomic traits.Furthermore,we showed that MsFTa2,a Flowering Locus T homolog,whose expression is upregulated in salt-resistant germplasms,may be associated with fall dormancy and salt resistance.Taken together,these genomic resources will facilitate alfalfa genetic research and agronomic improvement.展开更多
Pancreatic cancer is the fourth most common cause of cancer-related deaths in the United States, with over 38000 deaths in 2013. The opportunity to detect pancreatic cancer while it is still curable is dependent on ou...Pancreatic cancer is the fourth most common cause of cancer-related deaths in the United States, with over 38000 deaths in 2013. The opportunity to detect pancreatic cancer while it is still curable is dependent on our ability to identify and screen high-risk populations before their symptoms arise. Risk factors for developing pancreatic cancer include multiple genetic syndromes as well as modifiable risk factors. Genetic conditions include hereditary breast and ovarian cancer syndrome, Lynch Syndrome, familial adenomatous polyposis, Peutz-Jeghers Syndrome, familial atypical multiple mole melanoma syndrome, hereditary pancreatitis, cystic fibrosis, and ataxia-telangiectasia; having a genetic predisposition can raise the risk of developing pancreatic cancer up to 132-fold over the general population. Modifiable risk factors, which include tobacco exposure, alcohol use, chronic pancreatitis, diet, obesity, diabetes mellitus, as well as certain abdominal surgeries and infections, have also been shown to increase the risk of pancreatic cancer development. Several largevolume centers have initiated such screening protocols, and consensus-based guidelines for screening high-riskgroups have recently been published. The focus of this review will be both the genetic and modifiable risk factors implicated in pancreatic cancer, as well as a review of screening strategies and their diagnostic yields.展开更多
A new dinosaur of Early Cretaceous age was recently discovered from the Mazongshan area of northwestern Gansu Province, China. The new dinosaur represents a new genus and species of Sauropoda, and is among the most ba...A new dinosaur of Early Cretaceous age was recently discovered from the Mazongshan area of northwestern Gansu Province, China. The new dinosaur represents a new genus and species of Sauropoda, and is among the most basal members of Titanosauria. Its finding also suggests that titanosaurs might have originated in Asia no later than the Early Cretaceous.展开更多
Quenched and tempered steels are prone to hydrogen induced cracking in the heat affected zone after welding. The use of austenitic stainless steel consumables to weld the above steel was the only available remedy beca...Quenched and tempered steels are prone to hydrogen induced cracking in the heat affected zone after welding. The use of austenitic stainless steel consumables to weld the above steel was the only available remedy because of higher solubility for hydrogen in austenitic phase. In this investigation, an attempt was made to determine a suitable consumable to replace expensive austenitic consumables. Two different consumables, namely, austenitie stain less steel and low hydrogen ferritic steel, were used to fabricate the joints by shielded metal are welding （SMAW） and flux cored arc welding （FCAW） processes. The joints fabricated by using low hydrogen ferritic steel consumables showed superior transverse tensile properties, whereas joints fabricated by using austenitic stainless steel consumables exhibited better impact toughness, irrespective of the welding process used. The SMAW joints exhibited superior mechanical and impact properties, irrespective of the consumables used, than their FCAW counterparts.展开更多
Ge Gen Decoction(GGD),a Traditional Chinese Medicine prescription,is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus(IAV)infection.However,the underlying mechanis...Ge Gen Decoction(GGD),a Traditional Chinese Medicine prescription,is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus(IAV)infection.However,the underlying mechanism of GGD against IAV infection remains unclear.In this study,we found that GGD had moderate anti-IAV activity in vitro.GGD was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization sta ge.In vivo,GGD treatment reduced the virus titers of lung tissue significantly and improved the survival rate,lung index,and pulmonary hist opathological changes in H1N1-infected mice.We observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the control.GGD treatment decreased the expression of TNF-αand improved Th1/Th2 immune balance to reduce the excessive immune response in H1N1-infected mice.Besides,the expression of the toll-like receptor 7 signaling pathway in H1N1-infected mice decreased after GGD treatment.Our results showed that GGD has anti-IAV activity and can modulate the immune system to relieve lung inflammation.展开更多
设计并提出一种超高频射频识别系统读写器设计的新方案。该读写器采用IntelR1000收发器芯片、W78E365微控器，符合ISO18000-6C和EPC global Gen2标准，工作频率为860-960MHz，读写距离在2～10m之间。同时给出读写器硬件系统的组成和软...设计并提出一种超高频射频识别系统读写器设计的新方案。该读写器采用IntelR1000收发器芯片、W78E365微控器，符合ISO18000-6C和EPC global Gen2标准，工作频率为860-960MHz，读写距离在2～10m之间。同时给出读写器硬件系统的组成和软件工作流程，并针对同时读取多张卡的情况进行分析，实现了防冲突算法。该读写器支持SSB-ASK和DSB-ASK双重调制方式，可根据需要改变使用天线的单、双模式。展开更多
This paper lists the accepted names and classification of marine fungi,updating the scheme presented in 2009.The classification includes 1,112 species(in 472 genera):Ascomycota 805(in 352 genera),Basidiomycota 21 spec...This paper lists the accepted names and classification of marine fungi,updating the scheme presented in 2009.The classification includes 1,112 species(in 472 genera):Ascomycota 805(in 352 genera),Basidiomycota 21 species(in 17 genera),Chytridiomycota and related phyla 26 species(in 13 genera),Zygomycota three(in two genera),Blastocladiomycota one species(one genus),asexual morphs of filamentous fungi 43(in 26 genera);and marine yeasts:Ascomycota 138 species(in 35 genera),Basidiomycota 75 species(in 26 genera).These fungi belong to 129 families and 65 orders.The Halosphaeriaceae remains the largest family of marine fungi with 141 species in 59 genera,while the most specious genera are Aspergillus(47 species),Penicillium(39 species)and the yeast genus Candida(64 species).The review includes details of recent higher order nomenclature changes,and accounts of new families,genera and species described over the past 5 years.展开更多
A new gigantic sauropod, Huabeisaurus allocotus gen. et sp. nov., about 20 m in length and 5 m in height, was discovered in the Upper Cretaceous Huiquanpu Formation, Tianzhen County, Shanxi Province. It is notably dif...A new gigantic sauropod, Huabeisaurus allocotus gen. et sp. nov., about 20 m in length and 5 m in height, was discovered in the Upper Cretaceous Huiquanpu Formation, Tianzhen County, Shanxi Province. It is notably different from Diplodocidae, Titanosauridae and Nemegtosauridae in the following aspects: the teeth are strong, peglike with a length ratio of the tooth crown to tooth root at about 3 to 1; the cervical vertebrae are long with forked spines; the spines in dorsal vertebrae are relatively high, unbifurcated; the caudal vertebrae are amphicoelous, with anterior neural spines and unbifurcated spines and chevrons; the femur is straight and long, narrow and flat and the tibia and fibula are long and flat. These characters show that the described genus should represent a new family, Huabeisauridae fam. nov. The discovery enriches the sauropod dinosaur record in China, and is quite significant to the study of the taxonomy, evolution, migration, extinction and palaeobiogeographic provincialism of the Late Cretaceous sauropod dinosaurs.展开更多
A new basal hadrosauriform dinosaur, Xuwulongyueluni gen. et sp. nov., is reported based on an articulated specimen including a complete cranium, almost complete axial skeleton, and complete left pelvic girdle. The sp...A new basal hadrosauriform dinosaur, Xuwulongyueluni gen. et sp. nov., is reported based on an articulated specimen including a complete cranium, almost complete axial skeleton, and complete left pelvic girdle. The specimen is recovered from the Early Cretaceous Yujingzi Basin in the Jiuquan area, Gansu Province of northwestern China. Xuwulong is among the most basal members of hadrosauriform dinosaurs, and distinguished by its unique lower jaw in having a V-shaped rostral end of the dentary in lateral view and a mandibular articular surface which levels with mid-height of the dentary ramus, as well as an enlarged and downturned prepubic process. Including Xuwulong, three basal hadrosauriforms have been discovered from the Early Cretaceous Xinminpu Group in the Mazongshan area, making it an important area to study the origin and early evolution of duck-billed dinosaurs.展开更多
Two zosterophyll plants are described from the Lower Devonian （Lochkovian） Xitun Formation of Qujing, Yunnan, China. Xitunia spinitheca gen. et sp. nov. has stalked sporangia laterally attached on the axis in a heli...Two zosterophyll plants are described from the Lower Devonian （Lochkovian） Xitun Formation of Qujing, Yunnan, China. Xitunia spinitheca gen. et sp. nov. has stalked sporangia laterally attached on the axis in a helical arrangement. Sporangia are dorsoventrally flattened and composed of two unequal valves; the adaxial valve is round in face view, while the abaxial valve is larger than the former, triangular or wedge-shaped, and radially bears long spiny appendages along the distal margin. Xitunia shows new variation of sporangial morphology within the zosterophylls. Zosterophyllum minorstachyum sp. nov. has K-shaped branchings at the basal parts and small-sized terminal spikes, which consist of round to elliptical sporangia arranged helically. This paper provides new data on the diversity of plant types during Lochkovian when rare vascular plants were reported. As for various species of Zosterophyllum in South China, their apparent evolutionary trend of features from the Late Silurian to Early Devonian （Emsian） is discussed.展开更多
A new compsognathid dinosaur, Sinocalliopteryx gigas gen. et sp. nov., is erected based on a complete skeleton from the Early Cretaceous Yixian Formation of western Liaoning, northeastern China. It shares the features...A new compsognathid dinosaur, Sinocalliopteryx gigas gen. et sp. nov., is erected based on a complete skeleton from the Early Cretaceous Yixian Formation of western Liaoning, northeastern China. It shares the features with Huaxiagnathus orientalis in having a manus as long as the humerus plus radius, very large and subequally long manual claws I and H, and reduced olecranon process on the ulna. But it differs from Huaxiagnathus orientalis in having the much large size, a very long maxillary process of premaxilla not extending the vertical level of the maxillary antorbital fossa, and the proportionally longer ulna and so on. Sinocalliopteryx gigas gen. et sp. nov. represents the largest species among the known compsognathid dinosaurs, suggesting the tendency of the body enlargement in compsognathids to some extent. The long filamentous integuments are attached to the whole body of this compsognathid, confirming that such integuments evolved firstly in the basal coelurosaurs. This new giant compsognathid was a fierce carnivorous theropod, as shown further by an incomplete dromaeosaurid leg inside its abdominal cavity.展开更多
A glucosidic indole-lignan conjugate with a novel carbon skeleton, named isatindolignanoside A（1）, was isolated from an aqueous extract of the Isatis indigotica roots ＂ban lan gen＂. Its structure was elucidated by...A glucosidic indole-lignan conjugate with a novel carbon skeleton, named isatindolignanoside A（1）, was isolated from an aqueous extract of the Isatis indigotica roots ＂ban lan gen＂. Its structure was elucidated by comprehensive analysis of spectroscopic data, including J-and NOESY correlation-based configurational analysis, circular dichroism（CD） data, enzyme hydrolysis, and theoretical ECD calculation. In a preliminary assay, compound 1 showed antiviral activity against Coxsackie virus B3. This compound is the first example of natural product having a structural feature of conjugation between indole and lignan units, and its biosynthetic pathway is postulated.展开更多
基金supported by the National Key Research&Development Program of China(2019YFD1002701)Agricultural Variety Improvemnt Project of Shandong Province(2019LZGC010)the Project for Extramural Scientists of the State Key Laboratory for Agrobiotech no logy(2020SKLAB6-15,2011SKLAB01-1).
文摘Alfalfa(Medicago sativa)is one of the most important forage crops in the world;however,its molecular genetics and breeding research are hindered due to the lack of a high-quality reference genome.Here,we report a de novo assembled 816-Mb high-quality,chromosome-level haploid genome sequence for‘Zhongmu No.1’alfalfa,a heterozygous autotetraploid.The contig N50 is 3.92 Mb,and 49165 genes are annotated in the genome.The alfalfa genome is estimated to have diverged from M.truncatula approximately 8 million years ago.Genomic population analysis of 162 alfalfa accessions revealed high genetic diversity,weak population structure,and extensive gene flow from wild to cultivated alfalfa.Genome-wide association studies identified many candidate genes associated with important agronomic traits.Furthermore,we showed that MsFTa2,a Flowering Locus T homolog,whose expression is upregulated in salt-resistant germplasms,may be associated with fall dormancy and salt resistance.Taken together,these genomic resources will facilitate alfalfa genetic research and agronomic improvement.
文摘Pancreatic cancer is the fourth most common cause of cancer-related deaths in the United States, with over 38000 deaths in 2013. The opportunity to detect pancreatic cancer while it is still curable is dependent on our ability to identify and screen high-risk populations before their symptoms arise. Risk factors for developing pancreatic cancer include multiple genetic syndromes as well as modifiable risk factors. Genetic conditions include hereditary breast and ovarian cancer syndrome, Lynch Syndrome, familial adenomatous polyposis, Peutz-Jeghers Syndrome, familial atypical multiple mole melanoma syndrome, hereditary pancreatitis, cystic fibrosis, and ataxia-telangiectasia; having a genetic predisposition can raise the risk of developing pancreatic cancer up to 132-fold over the general population. Modifiable risk factors, which include tobacco exposure, alcohol use, chronic pancreatitis, diet, obesity, diabetes mellitus, as well as certain abdominal surgeries and infections, have also been shown to increase the risk of pancreatic cancer development. Several largevolume centers have initiated such screening protocols, and consensus-based guidelines for screening high-riskgroups have recently been published. The focus of this review will be both the genetic and modifiable risk factors implicated in pancreatic cancer, as well as a review of screening strategies and their diagnostic yields.
文摘A new dinosaur of Early Cretaceous age was recently discovered from the Mazongshan area of northwestern Gansu Province, China. The new dinosaur represents a new genus and species of Sauropoda, and is among the most basal members of Titanosauria. Its finding also suggests that titanosaurs might have originated in Asia no later than the Early Cretaceous.
文摘Quenched and tempered steels are prone to hydrogen induced cracking in the heat affected zone after welding. The use of austenitic stainless steel consumables to weld the above steel was the only available remedy because of higher solubility for hydrogen in austenitic phase. In this investigation, an attempt was made to determine a suitable consumable to replace expensive austenitic consumables. Two different consumables, namely, austenitie stain less steel and low hydrogen ferritic steel, were used to fabricate the joints by shielded metal are welding （SMAW） and flux cored arc welding （FCAW） processes. The joints fabricated by using low hydrogen ferritic steel consumables showed superior transverse tensile properties, whereas joints fabricated by using austenitic stainless steel consumables exhibited better impact toughness, irrespective of the welding process used. The SMAW joints exhibited superior mechanical and impact properties, irrespective of the consumables used, than their FCAW counterparts.
基金supported by the Key R&D Project in Shandong Province(the Second Development of the Chinese Patent Medicine Ge Gen Decoction Granule,No.2016CYJS08A01-8)
文摘Ge Gen Decoction(GGD),a Traditional Chinese Medicine prescription,is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus(IAV)infection.However,the underlying mechanism of GGD against IAV infection remains unclear.In this study,we found that GGD had moderate anti-IAV activity in vitro.GGD was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization sta ge.In vivo,GGD treatment reduced the virus titers of lung tissue significantly and improved the survival rate,lung index,and pulmonary hist opathological changes in H1N1-infected mice.We observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the control.GGD treatment decreased the expression of TNF-αand improved Th1/Th2 immune balance to reduce the excessive immune response in H1N1-infected mice.Besides,the expression of the toll-like receptor 7 signaling pathway in H1N1-infected mice decreased after GGD treatment.Our results showed that GGD has anti-IAV activity and can modulate the immune system to relieve lung inflammation.
文摘设计并提出一种超高频射频识别系统读写器设计的新方案。该读写器采用IntelR1000收发器芯片、W78E365微控器，符合ISO18000-6C和EPC global Gen2标准，工作频率为860-960MHz，读写距离在2～10m之间。同时给出读写器硬件系统的组成和软件工作流程，并针对同时读取多张卡的情况进行分析，实现了防冲突算法。该读写器支持SSB-ASK和DSB-ASK双重调制方式，可根据需要改变使用天线的单、双模式。
基金Gareth Jones is supported by the Distinguished Scientist Fellowship Program(DSFP),King Saud University.This Project was funded by the National Plan for Science,Technology and Innovation(MAARIFAH),King Abdulaziz City for Science and Technology,Kingdom of Saudi Arabia,Award Number(12-BIO2840-02.K.L).Pang would like to thank Ministry of Science and Technology,Taiwan for financial support(NSC101-2621-B-019-001-MY3).T.Boekhout is supported by a grant NPRP6-647-1-127 from the Qatar National Research Fund(a member of the Qatar Foundation).This research was also financially supported by The Biodiversity and Training Program(BRT R_251006,BRT R_351004,BRT_R352015)and National Center for Genetic Engineering and Biotechnology(BIOTEC),Thailand.We thank Drs.Holger Thüs and Patrick M.McCarthy for their comments on what constitutes littoral/intertidal lichensDrs.Joyce Longcore,D.Reham Simmons,Serygey K.Karpov,Frank Gleason for their critical comments on marine chytrids+1 种基金Drs.Teppo Rämä,Hans-Otto Baral and Ove E.Eriksson for allowing us to refer to their publication on Orbilia marina.Jack Fell for useful comments on marine yeastsRhiannon Owen for assistance with theses held at the Hugh Lloyd Library,University of Aberystwyth,Wales.
文摘This paper lists the accepted names and classification of marine fungi,updating the scheme presented in 2009.The classification includes 1,112 species(in 472 genera):Ascomycota 805(in 352 genera),Basidiomycota 21 species(in 17 genera),Chytridiomycota and related phyla 26 species(in 13 genera),Zygomycota three(in two genera),Blastocladiomycota one species(one genus),asexual morphs of filamentous fungi 43(in 26 genera);and marine yeasts:Ascomycota 138 species(in 35 genera),Basidiomycota 75 species(in 26 genera).These fungi belong to 129 families and 65 orders.The Halosphaeriaceae remains the largest family of marine fungi with 141 species in 59 genera,while the most specious genera are Aspergillus(47 species),Penicillium(39 species)and the yeast genus Candida(64 species).The review includes details of recent higher order nomenclature changes,and accounts of new families,genera and species described over the past 5 years.
文摘A new gigantic sauropod, Huabeisaurus allocotus gen. et sp. nov., about 20 m in length and 5 m in height, was discovered in the Upper Cretaceous Huiquanpu Formation, Tianzhen County, Shanxi Province. It is notably different from Diplodocidae, Titanosauridae and Nemegtosauridae in the following aspects: the teeth are strong, peglike with a length ratio of the tooth crown to tooth root at about 3 to 1; the cervical vertebrae are long with forked spines; the spines in dorsal vertebrae are relatively high, unbifurcated; the caudal vertebrae are amphicoelous, with anterior neural spines and unbifurcated spines and chevrons; the femur is straight and long, narrow and flat and the tibia and fibula are long and flat. These characters show that the described genus should represent a new family, Huabeisauridae fam. nov. The discovery enriches the sauropod dinosaur record in China, and is quite significant to the study of the taxonomy, evolution, migration, extinction and palaeobiogeographic provincialism of the Late Cretaceous sauropod dinosaurs.
基金provided by the National Natural Science Foundation of China(41072019 and 90914003)the basic outlay of scientific research work from Ministry of Science and Technology(J1003)+1 种基金National Science Foundation (1024671)Gansu Provincial Bureau of Geo-Exploration and Mineral Development,and Gansu Geological Museum
文摘A new basal hadrosauriform dinosaur, Xuwulongyueluni gen. et sp. nov., is reported based on an articulated specimen including a complete cranium, almost complete axial skeleton, and complete left pelvic girdle. The specimen is recovered from the Early Cretaceous Yujingzi Basin in the Jiuquan area, Gansu Province of northwestern China. Xuwulong is among the most basal members of hadrosauriform dinosaurs, and distinguished by its unique lower jaw in having a V-shaped rostral end of the dentary in lateral view and a mandibular articular surface which levels with mid-height of the dentary ramus, as well as an enlarged and downturned prepubic process. Including Xuwulong, three basal hadrosauriforms have been discovered from the Early Cretaceous Xinminpu Group in the Mazongshan area, making it an important area to study the origin and early evolution of duck-billed dinosaurs.
基金supportedby the National Natural Science Foundation of China(Nos.40672001,40802003,40830211)China PostdoctoralScience Foundation(20070420245)
文摘Two zosterophyll plants are described from the Lower Devonian （Lochkovian） Xitun Formation of Qujing, Yunnan, China. Xitunia spinitheca gen. et sp. nov. has stalked sporangia laterally attached on the axis in a helical arrangement. Sporangia are dorsoventrally flattened and composed of two unequal valves; the adaxial valve is round in face view, while the abaxial valve is larger than the former, triangular or wedge-shaped, and radially bears long spiny appendages along the distal margin. Xitunia shows new variation of sporangial morphology within the zosterophylls. Zosterophyllum minorstachyum sp. nov. has K-shaped branchings at the basal parts and small-sized terminal spikes, which consist of round to elliptical sporangia arranged helically. This paper provides new data on the diversity of plant types during Lochkovian when rare vascular plants were reported. As for various species of Zosterophyllum in South China, their apparent evolutionary trend of features from the Late Silurian to Early Devonian （Emsian） is discussed.
基金This research was supported by the National Basic Research Program of China （973 Project, grant no. 2006CB701405）the National Natural Science Foundation of China （grant no. 40272008）the China Geological Survey.
文摘A new compsognathid dinosaur, Sinocalliopteryx gigas gen. et sp. nov., is erected based on a complete skeleton from the Early Cretaceous Yixian Formation of western Liaoning, northeastern China. It shares the features with Huaxiagnathus orientalis in having a manus as long as the humerus plus radius, very large and subequally long manual claws I and H, and reduced olecranon process on the ulna. But it differs from Huaxiagnathus orientalis in having the much large size, a very long maxillary process of premaxilla not extending the vertical level of the maxillary antorbital fossa, and the proportionally longer ulna and so on. Sinocalliopteryx gigas gen. et sp. nov. represents the largest species among the known compsognathid dinosaurs, suggesting the tendency of the body enlargement in compsognathids to some extent. The long filamentous integuments are attached to the whole body of this compsognathid, confirming that such integuments evolved firstly in the basal coelurosaurs. This new giant compsognathid was a fierce carnivorous theropod, as shown further by an incomplete dromaeosaurid leg inside its abdominal cavity.
基金Financial support from the National Natural Science Foundation of China (Nos. 81373287, 81630094, and 30825044) is acknowledged
文摘A glucosidic indole-lignan conjugate with a novel carbon skeleton, named isatindolignanoside A（1）, was isolated from an aqueous extract of the Isatis indigotica roots ＂ban lan gen＂. Its structure was elucidated by comprehensive analysis of spectroscopic data, including J-and NOESY correlation-based configurational analysis, circular dichroism（CD） data, enzyme hydrolysis, and theoretical ECD calculation. In a preliminary assay, compound 1 showed antiviral activity against Coxsackie virus B3. This compound is the first example of natural product having a structural feature of conjugation between indole and lignan units, and its biosynthetic pathway is postulated.
