Our previous studies have confirmed that during nerve transposition repair to injured peripheral nerves, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively repair dis...Our previous studies have confirmed that during nerve transposition repair to injured peripheral nerves, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively repair distal nerve and target muscle tissue and restore muscle motor function. To observe the effect of nerve regeneration and motor function recovery after several types of nerve transposition for median nerve defect(2 mm), 30 Sprague-Dawley rats were randomly divided into sham operation group, epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group. Three months after nerve repair, the wrist flexion test was used to evaluate the recovery of wrist flexion after regeneration of median nerve in the affected limbs of rats. The number of myelinated nerve fibers, the thickness of myelin sheath, the diameter of axons and the cross-sectional area of axons in the proximal and distal segments of the repaired nerves were measured by osmic acid staining. The ratio of newly produced distal myelinated nerve fibers to the number of proximal myelinated nerve fibers was calculated. Wet weights of the flexor digitorum superficialis muscles were measured. Muscle fiber morphology was detected using hematoxylin-eosin staining. The cross-sectional area of muscle fibers was calculated to assess the recovery of muscles. Results showed that wrist flexion function was restored, and the nerve grew into the distal effector in all three nerve transposition groups and the epineurial neurorrhaphy group. There were differences in the number of myelinated nerve fibers in each group. The magnification of proximal to distal nerves was 1.80, 3.00, 2.50, and 3.12 in epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group, respectively. Nevertheless, axon diameters of new nerve fibers, cross-sectional areas of axons, t展开更多
Peripheral nerve injury is a common clinical problem because the dysfunction of distal limb seriously affects patients＇ quality of life,and it results in a huge social and economic burden.[1] Repair of peripheral ner...Peripheral nerve injury is a common clinical problem because the dysfunction of distal limb seriously affects patients＇ quality of life,and it results in a huge social and economic burden.[1] Repair of peripheral nerve injury is a very complex pathological process.Due to slow nerve regeneration,Wallerian degeneration of nerve stump,tissue adhesion,atrophy of muscles and motor end plates and other constraints,functional rehabilitation of the damaged nerve is always restricted.展开更多
目的研究全身振动治疗（WBVT）对兔膝骨关节炎软骨下骨结构与功能重塑的影响。方法新西兰兔24只，随机分为膝关节前交叉韧带切断术（ACLT组）和WBVT＋ACLT组，每组12只。两组行左侧ACLT，制备膝骨关节炎模型。术后2个月wBVT＋ACLT组实施...目的研究全身振动治疗（WBVT）对兔膝骨关节炎软骨下骨结构与功能重塑的影响。方法新西兰兔24只，随机分为膝关节前交叉韧带切断术（ACLT组）和WBVT＋ACLT组，每组12只。两组行左侧ACLT，制备膝骨关节炎模型。术后2个月wBVT＋ACLT组实施WBVT。振动治疗参数为：频率40Hz，振动幅度2～4mm，40min／d，5d／周，持续治疗4周。治疗完成后，取两组动物左侧膝股骨和胫骨置于Micro—CT系统进行扫描，应用Micview V2．1．2三维重建处理软件和ABA专用骨骼分析软件对股骨髁和胫骨平台骨小梁体积分数（BVF）、骨小梁厚度（Tb．Th）、骨小梁分离度（Tb．Sp）、骨小梁数量（Tb．N）、体积骨密度（vBMD）和组织骨密度（tBMD）进行检测。应用Geomagic Studio 11．0软件换算股骨髁和胫骨平台弹性模量（EM）、反应力（RF）和平均Von Miss应力（VMF）。结果显微CT成像结果发现：ACLT组软骨下骨骨小梁出现稀疏表现并呈现断裂现象，部分骨小梁排列紊乱，结构扭曲。关节边缘处见不同程度的椭圆形或圆形骨赘形成。WBVT＋ACLT组软骨下骨骨小梁虽然也出现稀疏断裂的现象，但直观上较ACI．T组骨小梁排列较为整齐有序，少见结构性扭曲。WBVT＋ACLT组与ACLT组相比较，BVF、Tb．N、Tb．Th，股骨的EM、RF和VMF升高（P〈0．05）；Tb．Sp减少（P〈0．05）。骨密度（vBMD和tBMD）增加（P〈0．05）。结论WBVT可有效改善兔膝骨关节炎早期阶段软骨下骨微结构和力学性能，起到保护进展性骨关节炎软骨下骨微损伤和力学性能降低的作用。展开更多
基金funded by the National Natural Science Foundation of China,No.31571236,31571235(to YHK,PXZ)National Key Research and Development Program of China,No.2016YFC1101604(to DYZ)+3 种基金National Key Basic Research Program of China(973 Program),No.2014CB542200(to BGJ)Ministry of Education Innovation Program of China,No.IRT_16R01(to BGJ)Beijing Science and Technology New Star Cross Program of China,No.2018019(to PXZ)Peking University People’s Hospital Research and Development Funds,No.RDH2017-01(to HLX)
文摘Our previous studies have confirmed that during nerve transposition repair to injured peripheral nerves, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively repair distal nerve and target muscle tissue and restore muscle motor function. To observe the effect of nerve regeneration and motor function recovery after several types of nerve transposition for median nerve defect(2 mm), 30 Sprague-Dawley rats were randomly divided into sham operation group, epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group. Three months after nerve repair, the wrist flexion test was used to evaluate the recovery of wrist flexion after regeneration of median nerve in the affected limbs of rats. The number of myelinated nerve fibers, the thickness of myelin sheath, the diameter of axons and the cross-sectional area of axons in the proximal and distal segments of the repaired nerves were measured by osmic acid staining. The ratio of newly produced distal myelinated nerve fibers to the number of proximal myelinated nerve fibers was calculated. Wet weights of the flexor digitorum superficialis muscles were measured. Muscle fiber morphology was detected using hematoxylin-eosin staining. The cross-sectional area of muscle fibers was calculated to assess the recovery of muscles. Results showed that wrist flexion function was restored, and the nerve grew into the distal effector in all three nerve transposition groups and the epineurial neurorrhaphy group. There were differences in the number of myelinated nerve fibers in each group. The magnification of proximal to distal nerves was 1.80, 3.00, 2.50, and 3.12 in epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group, respectively. Nevertheless, axon diameters of new nerve fibers, cross-sectional areas of axons, t
基金This research was continuously funded by grants from Chinese National Key Basic Research 973 Program （No. 2014CB542201）, Chinese National High Technology Research and Development 863 Program （No. SS2015AA020501）, and Chinese National General Program of National Natural Science Foundation （Nos. 31771322, 31571235, 31571236, 31271284, 31171150, 81171146, 31471144, 30971526, 31100860, 31040043, 31371210, 81372044）.
文摘Peripheral nerve injury is a common clinical problem because the dysfunction of distal limb seriously affects patients＇ quality of life,and it results in a huge social and economic burden.[1] Repair of peripheral nerve injury is a very complex pathological process.Due to slow nerve regeneration,Wallerian degeneration of nerve stump,tissue adhesion,atrophy of muscles and motor end plates and other constraints,functional rehabilitation of the damaged nerve is always restricted.
文摘目的研究全身振动治疗（WBVT）对兔膝骨关节炎软骨下骨结构与功能重塑的影响。方法新西兰兔24只，随机分为膝关节前交叉韧带切断术（ACLT组）和WBVT＋ACLT组，每组12只。两组行左侧ACLT，制备膝骨关节炎模型。术后2个月wBVT＋ACLT组实施WBVT。振动治疗参数为：频率40Hz，振动幅度2～4mm，40min／d，5d／周，持续治疗4周。治疗完成后，取两组动物左侧膝股骨和胫骨置于Micro—CT系统进行扫描，应用Micview V2．1．2三维重建处理软件和ABA专用骨骼分析软件对股骨髁和胫骨平台骨小梁体积分数（BVF）、骨小梁厚度（Tb．Th）、骨小梁分离度（Tb．Sp）、骨小梁数量（Tb．N）、体积骨密度（vBMD）和组织骨密度（tBMD）进行检测。应用Geomagic Studio 11．0软件换算股骨髁和胫骨平台弹性模量（EM）、反应力（RF）和平均Von Miss应力（VMF）。结果显微CT成像结果发现：ACLT组软骨下骨骨小梁出现稀疏表现并呈现断裂现象，部分骨小梁排列紊乱，结构扭曲。关节边缘处见不同程度的椭圆形或圆形骨赘形成。WBVT＋ACLT组软骨下骨骨小梁虽然也出现稀疏断裂的现象，但直观上较ACI．T组骨小梁排列较为整齐有序，少见结构性扭曲。WBVT＋ACLT组与ACLT组相比较，BVF、Tb．N、Tb．Th，股骨的EM、RF和VMF升高（P〈0．05）；Tb．Sp减少（P〈0．05）。骨密度（vBMD和tBMD）增加（P〈0．05）。结论WBVT可有效改善兔膝骨关节炎早期阶段软骨下骨微结构和力学性能，起到保护进展性骨关节炎软骨下骨微损伤和力学性能降低的作用。
