目的探究蒙山地区地椒精油对淡色库蚊幼虫和成蚊的生物活性并对其成分进行分析,为植物源杀虫剂的研制提供理论依据。方法用挥发油提取器提取地椒精油,采用幼虫浸渍法测定地椒精油对淡色库蚊4龄幼虫的杀灭活性,采用密闭三角瓶熏蒸法测定...目的探究蒙山地区地椒精油对淡色库蚊幼虫和成蚊的生物活性并对其成分进行分析,为植物源杀虫剂的研制提供理论依据。方法用挥发油提取器提取地椒精油,采用幼虫浸渍法测定地椒精油对淡色库蚊4龄幼虫的杀灭活性,采用密闭三角瓶熏蒸法测定对成蚊的熏蒸活性,通过气相色谱-质谱法(GC-MS)分析地椒精油的化学成分。采用SPSS 25.0软件分析,计算半数致死浓度(LC50)、击倒中时(KT50)、毒力回归方程及95%置信限并进行卡方检验。结果幼虫杀灭活性测定显示地椒精油对淡色库蚊4龄幼虫的24 h LC50值为104.74μg/mL(χ^(2)=0.787,P=0.853);雌性成蚊熏蒸活性的KT_(50)值为20.73 min,差异有统计学意义。地椒精油中共检测出50种化合物,其中γ-松油烯的相对含量最高(12.18%),其次是左旋龙脑(8.56%)、3,4,5-三甲基甲苯(7.39%)和麝香草酚(6.04%)。结论蒙山地区地椒精油对淡色库蚊幼虫和成蚊均具有一定的杀虫活性,有望作为潜在的杀虫剂。展开更多
Objective: This paper mainly determined the action modes of extract of Aloe vera L. against Tetranychus cinnabarinus Boisduval. Methods: The different action modes, contact action, repellent, fumigant, and oviposition...Objective: This paper mainly determined the action modes of extract of Aloe vera L. against Tetranychus cinnabarinus Boisduval. Methods: The different action modes, contact action, repellent, fumigant, and oviposition inhibition property of the acetone extract of Aloe vera L. leaf against the carmine spider mite Tetranychus cinnabaribus (Boisduval) (Acarina: Tetranychidae) were investigated at 26°C ± 1°C, 75% - 80% relative humidity, and 14:10 light: day cycle in the laboratory. Results: Based on the established toxicity regression line of the Aloe vera L. acetone extract against female adult mites, the median lethal concentrations (LC50) were 0.836 and 0.167 mg/mL for 48 and 72 h, respectively. With processing time increased, the contact acaricidal activity increased and the repellent activity gradually decreased. The main modes of action of the extract against female adult mites were contact and repellent, and preferable effects were observed on adult mites. These results indicate that A. vera L. extract contains acaricidal and repellent bioactive components that may be useful in future control of the phytophagous mites.展开更多
文摘目的探究蒙山地区地椒精油对淡色库蚊幼虫和成蚊的生物活性并对其成分进行分析,为植物源杀虫剂的研制提供理论依据。方法用挥发油提取器提取地椒精油,采用幼虫浸渍法测定地椒精油对淡色库蚊4龄幼虫的杀灭活性,采用密闭三角瓶熏蒸法测定对成蚊的熏蒸活性,通过气相色谱-质谱法(GC-MS)分析地椒精油的化学成分。采用SPSS 25.0软件分析,计算半数致死浓度(LC50)、击倒中时(KT50)、毒力回归方程及95%置信限并进行卡方检验。结果幼虫杀灭活性测定显示地椒精油对淡色库蚊4龄幼虫的24 h LC50值为104.74μg/mL(χ^(2)=0.787,P=0.853);雌性成蚊熏蒸活性的KT_(50)值为20.73 min,差异有统计学意义。地椒精油中共检测出50种化合物,其中γ-松油烯的相对含量最高(12.18%),其次是左旋龙脑(8.56%)、3,4,5-三甲基甲苯(7.39%)和麝香草酚(6.04%)。结论蒙山地区地椒精油对淡色库蚊幼虫和成蚊均具有一定的杀虫活性,有望作为潜在的杀虫剂。
文摘Objective: This paper mainly determined the action modes of extract of Aloe vera L. against Tetranychus cinnabarinus Boisduval. Methods: The different action modes, contact action, repellent, fumigant, and oviposition inhibition property of the acetone extract of Aloe vera L. leaf against the carmine spider mite Tetranychus cinnabaribus (Boisduval) (Acarina: Tetranychidae) were investigated at 26°C ± 1°C, 75% - 80% relative humidity, and 14:10 light: day cycle in the laboratory. Results: Based on the established toxicity regression line of the Aloe vera L. acetone extract against female adult mites, the median lethal concentrations (LC50) were 0.836 and 0.167 mg/mL for 48 and 72 h, respectively. With processing time increased, the contact acaricidal activity increased and the repellent activity gradually decreased. The main modes of action of the extract against female adult mites were contact and repellent, and preferable effects were observed on adult mites. These results indicate that A. vera L. extract contains acaricidal and repellent bioactive components that may be useful in future control of the phytophagous mites.
