The balance of redox homeostasis is key to stem cell maintenance and differentiation.However,this balance is disrupted by the overproduced reactive oxygen species(ROS)in pathological conditions,which seriously impair ...The balance of redox homeostasis is key to stem cell maintenance and differentiation.However,this balance is disrupted by the overproduced reactive oxygen species(ROS)in pathological conditions,which seriously impair the therapeutic efficacy of stem cells.In the present study,highly dispersed fullerol nanocrystals with enhanced bioreactivity were incorporated into hydrogel microspheres using one-step innovative microfluidic technology to construct fullerol-hydrogel microfluidic spheres(FMSs)for in situ regulating the redox homeostasis of stem cells and promoting refractory bone healing.It was demonstrated that FMSs exhibited excellent antioxidant activity to quench both intracellular and extracellular ROS,sparing stem cells from oxidative stress damage.Furthermore,these could effectively promote the osteogenic differentiation of stem cells with the activation of FoxO1 signaling,indicating the intrinsically osteogenic property of FMSs.By injecting the stem cells-laden FMSs into rat calvarial defects,the formation of new bone was remarkably reinforced,which is a positive synergic effect from modulating the ROS microenvironment and enhancing the osteogenesis of stem cells.Collectively,the antioxidative FMSs,as injectable stem cell carriers,hold enormous promise for refractory bone healing,which can also be expanded to deliver a variety of other cells,targeting diseases that require in situ redox regulation.展开更多
A new C 60 derivative hydroxylated fullerene epoxide [C 60 (OH) x (O) y ] was prepared by the reaction of C 60 benzene solution with aqueous NaOH and H 2O 2 in the presence of tetrabutylammoni...A new C 60 derivative hydroxylated fullerene epoxide [C 60 (OH) x (O) y ] was prepared by the reaction of C 60 benzene solution with aqueous NaOH and H 2O 2 in the presence of tetrabutylammonium hydroxide(TBAH) as the catalyzer. The structure of hydroxylated fullerene epoxide was characterized by FT IR, NMR and MS , and it was found that it is easy to hydrolyze to fullerol.展开更多
Fullerols of C 60 and of C 70 \ [C 60 (OH)\-\%n\%, C 70 (OH)\-\%m\%], water\|soluble fullerene derivatives, unlike some other fullerene derivatives (such as C 60 (C\-4H\-6O), C 60 (C\-3H\-7N) and C 60 [C(COOEt)\-2]\-\...Fullerols of C 60 and of C 70 \ [C 60 (OH)\-\%n\%, C 70 (OH)\-\%m\%], water\|soluble fullerene derivatives, unlike some other fullerene derivatives (such as C 60 (C\-4H\-6O), C 60 (C\-3H\-7N) and C 60 [C(COOEt)\-2]\-\%x\%), do not result in excited triplet state but in ionization via monophotonic process in aqueous solutions with 248 nm laser. The quantum yields of formation of hydrated electron ( Φ e - ) are determined to be 0.08 and 0.11 for fullerols of C 60 and of C 70 respectively at room temperature (ca. 15℃) with KI solution used as reference. By laser flash photolysis and oxidation of sulfate radical anion SO -· \-4, the fullerol radical cation or neutral radical of C 60 is confirmed to be existent and the transient absorption spectra of fullerol radical cation of C 70 are observed for the first time.展开更多
A dialysis-based method was developed to remove Na ions in fullerol preparation by a conventional synthetic route. Fullerol was synthesized using a typical two-phase reaction in the presence of tetrabutylammonium hydr...A dialysis-based method was developed to remove Na ions in fullerol preparation by a conventional synthetic route. Fullerol was synthesized using a typical two-phase reaction in the presence of tetrabutylammonium hydroxide. Its structure was confirmed by UV spectra, Fourier transform infrared spectroscopy spectra, 1 H nuclear magnetic resonance, and matrix-assisted laser desorption/ionization time of flight mass spectra. The content of Na element was determined by inductive coupled plasma atomic emission spectroscopy. The mass content of Na in the product obtained from the conventional route was about 10%, and it could be almost completely eliminated by dialysis with a membrane of molecular weight cut-off (MWCO) 8-15 kDa. The yield of fullerol by the dialysis method was slightly higher than by the conventional route. These results indicate that dialysis may have a potential application for fullerol preparation.展开更多
开发了新的富勒醇、异硫氰酸荧光素和N,N-二甲基苯胺(F-ol-(FITC)n-DMA)标记试剂.基于F-ol的活性-OH与FITC游离的-COOH反应生成含有多个FITC分子的复合物(F-ol-(FITC)n-DMA),增加WGA-AP-WGA-F-ol FITC-DMA(WGA和AP分别为麦胚凝集素与碱...开发了新的富勒醇、异硫氰酸荧光素和N,N-二甲基苯胺(F-ol-(FITC)n-DMA)标记试剂.基于F-ol的活性-OH与FITC游离的-COOH反应生成含有多个FITC分子的复合物(F-ol-(FITC)n-DMA),增加WGA-AP-WGA-F-ol FITC-DMA(WGA和AP分别为麦胚凝集素与碱性磷酸酶)生物靶的发光分子数而进一步提高SSRTP(固体基质室温磷光法)的灵敏度.用F-ol-(FITC)n-DMA标记WGA方法的检出限为0.15 ag spot-1(F-ol)与0.097 ag spot-1((FITC),比F-ol-DMA、FITC-DEA单发光分子标记WGA方法的检出限(0.20 ag spot-1(F-ol),0.22 ag spot-1(FITC)低.用于血清试样中AP含量的测定结果与酶联免疫法相吻合.展开更多
基金This work was supported by the National Key Research and Development Program of China(2020YFA0908200)National Natural Science Foundation of China(81772372 and 81930051)+2 种基金Shanghai Jiao Tong University“Medical and Research”Program(ZH2018ZDA04)Science and Technology Commission of Shanghai Municipality(18140901500,19440760400)Shanghai Municipal Health and Family Planning Commission(201840027).
文摘The balance of redox homeostasis is key to stem cell maintenance and differentiation.However,this balance is disrupted by the overproduced reactive oxygen species(ROS)in pathological conditions,which seriously impair the therapeutic efficacy of stem cells.In the present study,highly dispersed fullerol nanocrystals with enhanced bioreactivity were incorporated into hydrogel microspheres using one-step innovative microfluidic technology to construct fullerol-hydrogel microfluidic spheres(FMSs)for in situ regulating the redox homeostasis of stem cells and promoting refractory bone healing.It was demonstrated that FMSs exhibited excellent antioxidant activity to quench both intracellular and extracellular ROS,sparing stem cells from oxidative stress damage.Furthermore,these could effectively promote the osteogenic differentiation of stem cells with the activation of FoxO1 signaling,indicating the intrinsically osteogenic property of FMSs.By injecting the stem cells-laden FMSs into rat calvarial defects,the formation of new bone was remarkably reinforced,which is a positive synergic effect from modulating the ROS microenvironment and enhancing the osteogenesis of stem cells.Collectively,the antioxidative FMSs,as injectable stem cell carriers,hold enormous promise for refractory bone healing,which can also be expanded to deliver a variety of other cells,targeting diseases that require in situ redox regulation.
文摘A new C 60 derivative hydroxylated fullerene epoxide [C 60 (OH) x (O) y ] was prepared by the reaction of C 60 benzene solution with aqueous NaOH and H 2O 2 in the presence of tetrabutylammonium hydroxide(TBAH) as the catalyzer. The structure of hydroxylated fullerene epoxide was characterized by FT IR, NMR and MS , and it was found that it is easy to hydrolyze to fullerol.
文摘Fullerols of C 60 and of C 70 \ [C 60 (OH)\-\%n\%, C 70 (OH)\-\%m\%], water\|soluble fullerene derivatives, unlike some other fullerene derivatives (such as C 60 (C\-4H\-6O), C 60 (C\-3H\-7N) and C 60 [C(COOEt)\-2]\-\%x\%), do not result in excited triplet state but in ionization via monophotonic process in aqueous solutions with 248 nm laser. The quantum yields of formation of hydrated electron ( Φ e - ) are determined to be 0.08 and 0.11 for fullerols of C 60 and of C 70 respectively at room temperature (ca. 15℃) with KI solution used as reference. By laser flash photolysis and oxidation of sulfate radical anion SO -· \-4, the fullerol radical cation or neutral radical of C 60 is confirmed to be existent and the transient absorption spectra of fullerol radical cation of C 70 are observed for the first time.
基金Supported by the National Natural Science Foundation of China （20672012） the Natural Science Foundation of Beijing（20822020）
文摘A dialysis-based method was developed to remove Na ions in fullerol preparation by a conventional synthetic route. Fullerol was synthesized using a typical two-phase reaction in the presence of tetrabutylammonium hydroxide. Its structure was confirmed by UV spectra, Fourier transform infrared spectroscopy spectra, 1 H nuclear magnetic resonance, and matrix-assisted laser desorption/ionization time of flight mass spectra. The content of Na element was determined by inductive coupled plasma atomic emission spectroscopy. The mass content of Na in the product obtained from the conventional route was about 10%, and it could be almost completely eliminated by dialysis with a membrane of molecular weight cut-off (MWCO) 8-15 kDa. The yield of fullerol by the dialysis method was slightly higher than by the conventional route. These results indicate that dialysis may have a potential application for fullerol preparation.
文摘开发了新的富勒醇、异硫氰酸荧光素和N,N-二甲基苯胺(F-ol-(FITC)n-DMA)标记试剂.基于F-ol的活性-OH与FITC游离的-COOH反应生成含有多个FITC分子的复合物(F-ol-(FITC)n-DMA),增加WGA-AP-WGA-F-ol FITC-DMA(WGA和AP分别为麦胚凝集素与碱性磷酸酶)生物靶的发光分子数而进一步提高SSRTP(固体基质室温磷光法)的灵敏度.用F-ol-(FITC)n-DMA标记WGA方法的检出限为0.15 ag spot-1(F-ol)与0.097 ag spot-1((FITC),比F-ol-DMA、FITC-DEA单发光分子标记WGA方法的检出限(0.20 ag spot-1(F-ol),0.22 ag spot-1(FITC)低.用于血清试样中AP含量的测定结果与酶联免疫法相吻合.
