An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intronⅠ at the site between +809 and +810 base. Based on these result...An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intronⅠ at the site between +809 and +810 base. Based on these results, a PCR programme was created to genotype animal individuals in different pig breeds at FSHβ locus and polymorphism of FSHβ gene was analyzed. With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco. Averagely the AA sows give more 1.5 piglets than BB sows do per litter.展开更多
Estrogen receptor (ESR) and Follicular-stimula-ting hormone beta subunit (FSHβ) genes were chosen as candidates to determine whether they control litter size and some other reproductive traits in swine. 269 sows from...Estrogen receptor (ESR) and Follicular-stimula-ting hormone beta subunit (FSHβ) genes were chosen as candidates to determine whether they control litter size and some other reproductive traits in swine. 269 sows from five different pig breeds were genotyped by an established PCR-RFLPs protocol at both ESR and FSHβ loci. The effects of both ESR and FSHβ on pig reproductive traits, including total number born (TNB) and number born alive (NBA), are analyzed by SAS software (version 6.12). These computation results demonstrated that both ESR locus and FSHβ locus are the major genes influencing litter size in pigs. The sows of BBBB combined genotype of ESR and FSHβ loci generally produce 1.85-3.01 TNB and 2.0-3.0 NBA more than those of ABAA combined genotype. The notable effect of ESR locus and FSHβ locus on litter size of pigs have made it possible to improve the pig reproduction by Marker-assisted selection (MAS). Moreover, introgression of the beneficial alleles into commercial pig breeding lines,展开更多
The objectives of the present study were to detect the polymorphism in follicle stimulating hormone beta （FSHβ） gene and investigate the relationship between FSHβ gene and high prolificacy in goats.According to th...The objectives of the present study were to detect the polymorphism in follicle stimulating hormone beta （FSHβ） gene and investigate the relationship between FSHβ gene and high prolificacy in goats.According to the sequence of ovine FSHβ gene,two pairs of primers were designed to detect single nucleotide polymorphism of exon 2 of FSHβ gene in two goat breeds,Xinong Saanen dairy goat and Boer goat,by PCR-SSCP.The least square mean and genetic variance of different genotypes at polymorphic locus were analyzed,and the selection reaction was forecasted by genetic correlation between marker locus and genetic variance of litter size traits.There was one polymorphic locus occurring in exon 2 （P2） with three genotypes （EE,EF and FF）.In Xinong Saanen dairy goat,EE genotype had significantly higher （P 〈 0.05） litter size than EF and FF genotype in the first to fourth parity and average parity;EF genotype had significantly higher （P 〈 0.05） litter size than FF genotype in the second and average parity.In Boer goat,EE genotype had significantly higher （P 〈 0.01） litter size than EF and FF genotype in the second to fourth parity and average parity;EF genotype had significantly higher （P 〈 0.05） litter size than FF genotype in average parity.In Xinong Saanen dairy and Boer goat,the heredity of litter size was mainly influenced by genetic additive effect and the EE genotype is a favorable marker genotype for litter size in these two breeds.展开更多
文摘An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intronⅠ at the site between +809 and +810 base. Based on these results, a PCR programme was created to genotype animal individuals in different pig breeds at FSHβ locus and polymorphism of FSHβ gene was analyzed. With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco. Averagely the AA sows give more 1.5 piglets than BB sows do per litter.
基金the National Natural Foundation for Outstanding Youth of China (Grant No. 39725022) the State Major Basic Research Development Program (Grant No. G20000161) the Trans-century Project for Science and Technology Head Training in Jiangxi Province.
文摘Estrogen receptor (ESR) and Follicular-stimula-ting hormone beta subunit (FSHβ) genes were chosen as candidates to determine whether they control litter size and some other reproductive traits in swine. 269 sows from five different pig breeds were genotyped by an established PCR-RFLPs protocol at both ESR and FSHβ loci. The effects of both ESR and FSHβ on pig reproductive traits, including total number born (TNB) and number born alive (NBA), are analyzed by SAS software (version 6.12). These computation results demonstrated that both ESR locus and FSHβ locus are the major genes influencing litter size in pigs. The sows of BBBB combined genotype of ESR and FSHβ loci generally produce 1.85-3.01 TNB and 2.0-3.0 NBA more than those of ABAA combined genotype. The notable effect of ESR locus and FSHβ locus on litter size of pigs have made it possible to improve the pig reproduction by Marker-assisted selection (MAS). Moreover, introgression of the beneficial alleles into commercial pig breeding lines,
基金supported by the National 863 Pro-gram of China (2007AA10Z167)the Key Technologies R&D Program of China during the Eleventh Five-Year Plan period (2006BAD04A11)
文摘The objectives of the present study were to detect the polymorphism in follicle stimulating hormone beta （FSHβ） gene and investigate the relationship between FSHβ gene and high prolificacy in goats.According to the sequence of ovine FSHβ gene,two pairs of primers were designed to detect single nucleotide polymorphism of exon 2 of FSHβ gene in two goat breeds,Xinong Saanen dairy goat and Boer goat,by PCR-SSCP.The least square mean and genetic variance of different genotypes at polymorphic locus were analyzed,and the selection reaction was forecasted by genetic correlation between marker locus and genetic variance of litter size traits.There was one polymorphic locus occurring in exon 2 （P2） with three genotypes （EE,EF and FF）.In Xinong Saanen dairy goat,EE genotype had significantly higher （P 〈 0.05） litter size than EF and FF genotype in the first to fourth parity and average parity;EF genotype had significantly higher （P 〈 0.05） litter size than FF genotype in the second and average parity.In Boer goat,EE genotype had significantly higher （P 〈 0.01） litter size than EF and FF genotype in the second to fourth parity and average parity;EF genotype had significantly higher （P 〈 0.05） litter size than FF genotype in average parity.In Xinong Saanen dairy and Boer goat,the heredity of litter size was mainly influenced by genetic additive effect and the EE genotype is a favorable marker genotype for litter size in these two breeds.
