Plants acclimate to drought optimizing the trade-off between biomass production and water loss while ensuring their survival and reproduction. Plants also modify their growth or phenology as complementary strategies i...Plants acclimate to drought optimizing the trade-off between biomass production and water loss while ensuring their survival and reproduction. Plants also modify their growth or phenology as complementary strategies in response to stress. Despite evidence of an interaction between flowering time and plant growth response to environmental stresses, this interaction in response to drought is under debate. To contribute to the analysis of this interaction, leaf growth of 35 genetically modified lines of Arabidopsis thaliana and their common wild-type, Col-0 was analyzed by a quantitative multi-scale phenotyping approach from cellular to whole plant scale both in well-watered and soil moderate water deficit conditions. These genotypes were selected for the various physiological functions potentially altered by their genetic modification and that could interact with plant growth and/or their drought responses. In all genotypes, leaf expansion decreased in response to drought both at the whole rosette and the individual leaf levels. Additionally, epidermal cell area and/or epidermal cell number decreased in response to the drought treatment. In contrast, the number of rosette leaves was reduced in only half of the genotypes and leaf growth duration was only modified in 4 of them. Despite long photoperiod conditions, the duration of the vegetative phase, i.e. the time elapsed between germination and flowering stage, varied from 12 to 27 days among genotypes under well watered conditions. Our analyses revealed that the differences of flowering time observed in well-watered condition impacted the leaf area response to drought. Early-flowering genotypes slightly decreased their final leaf number, but strongly reduced their individual leaf area compared with the late-flowering ones. This result underlines the difficulty to analyze plant response to environmental stresses when genotypes with different flowering dates are compared.展开更多
A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicu...A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kgP·ha-1. Both treatments were replicated four times in a split plot design. The following parameters were measured: plant height (cm), number leaves per plant, number of days to 50% flowering, number of days to 50% pod formation, stem girth and leaf area (LA). Results showed that inoculation with B. japonicum significantly increased plant height, number of leaves, leaf chlorophyll content, stem girth, leaf area (LA) and leaf area index (LAI). However, Phosphorus supplementation had significant effects in some parameters measured. The use of effective strain of B. japonicum and phosphorus supplementation was an efficient way of enhancing the growth of soybean.展开更多
Central Nepal has long history in the field of botanical investigation in contrast with different parts of the nation. Nevertheless, the present study area (Manaslu Conservation Area) is still under-explored. The pres...Central Nepal has long history in the field of botanical investigation in contrast with different parts of the nation. Nevertheless, the present study area (Manaslu Conservation Area) is still under-explored. The present floristic study was carried out with the aim of fulfilling this gap by giving particular attention on flowering plants and their documentation. Two field visits were directed in May-June 2012 and September-October 2012 in two unique seasons with a specific end goal to gather plant samples of both seasons. On the contrary, herbarium specimens were prepared and later identified at Tribhuvan University Central Herbarium (TUCH) and National Herbarium and Plant Laboratory (KATH). The present study uncovers the presence of 276 species of flowering plants belonging to 78 families and 200 genera of which 63 families belong to dicotyledons and 15 families belong to monocotyledons. Among these, 17 species (6.15%) of climbers, 178 species (64.49%) of herbs, 58 species (21.01%) of shrubs and 23 species (8.33%) of trees are found. Economically important flowering plants comprise about 156 species where 75 species are used as medicine, 29 species as fuel wood, 23 species as fodder, 29 species are edible, 6 species are for religious purpose, 5 specifically for construction purpose, 10 for ornamental and 13 species reported to have miscellaneous uses. MCA accounts to 9 flowering plant species for research and development and agro-technology development and 12 species are listed under IUCN and CAMP threatened categories. MCA and its affinity are likewise house to 10 endemic flowering plants. As from the study it can be inferred that Manaslu Conservation Area (MCA) is a treasure hub of flowering plants that not only represent its economic importance rather it is also a house to threaten and endemic flowering plants. Thus, proper management plans must be implemented for the conservation of resources in MCA.展开更多
文摘Plants acclimate to drought optimizing the trade-off between biomass production and water loss while ensuring their survival and reproduction. Plants also modify their growth or phenology as complementary strategies in response to stress. Despite evidence of an interaction between flowering time and plant growth response to environmental stresses, this interaction in response to drought is under debate. To contribute to the analysis of this interaction, leaf growth of 35 genetically modified lines of Arabidopsis thaliana and their common wild-type, Col-0 was analyzed by a quantitative multi-scale phenotyping approach from cellular to whole plant scale both in well-watered and soil moderate water deficit conditions. These genotypes were selected for the various physiological functions potentially altered by their genetic modification and that could interact with plant growth and/or their drought responses. In all genotypes, leaf expansion decreased in response to drought both at the whole rosette and the individual leaf levels. Additionally, epidermal cell area and/or epidermal cell number decreased in response to the drought treatment. In contrast, the number of rosette leaves was reduced in only half of the genotypes and leaf growth duration was only modified in 4 of them. Despite long photoperiod conditions, the duration of the vegetative phase, i.e. the time elapsed between germination and flowering stage, varied from 12 to 27 days among genotypes under well watered conditions. Our analyses revealed that the differences of flowering time observed in well-watered condition impacted the leaf area response to drought. Early-flowering genotypes slightly decreased their final leaf number, but strongly reduced their individual leaf area compared with the late-flowering ones. This result underlines the difficulty to analyze plant response to environmental stresses when genotypes with different flowering dates are compared.
文摘A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kgP·ha-1. Both treatments were replicated four times in a split plot design. The following parameters were measured: plant height (cm), number leaves per plant, number of days to 50% flowering, number of days to 50% pod formation, stem girth and leaf area (LA). Results showed that inoculation with B. japonicum significantly increased plant height, number of leaves, leaf chlorophyll content, stem girth, leaf area (LA) and leaf area index (LAI). However, Phosphorus supplementation had significant effects in some parameters measured. The use of effective strain of B. japonicum and phosphorus supplementation was an efficient way of enhancing the growth of soybean.
文摘Central Nepal has long history in the field of botanical investigation in contrast with different parts of the nation. Nevertheless, the present study area (Manaslu Conservation Area) is still under-explored. The present floristic study was carried out with the aim of fulfilling this gap by giving particular attention on flowering plants and their documentation. Two field visits were directed in May-June 2012 and September-October 2012 in two unique seasons with a specific end goal to gather plant samples of both seasons. On the contrary, herbarium specimens were prepared and later identified at Tribhuvan University Central Herbarium (TUCH) and National Herbarium and Plant Laboratory (KATH). The present study uncovers the presence of 276 species of flowering plants belonging to 78 families and 200 genera of which 63 families belong to dicotyledons and 15 families belong to monocotyledons. Among these, 17 species (6.15%) of climbers, 178 species (64.49%) of herbs, 58 species (21.01%) of shrubs and 23 species (8.33%) of trees are found. Economically important flowering plants comprise about 156 species where 75 species are used as medicine, 29 species as fuel wood, 23 species as fodder, 29 species are edible, 6 species are for religious purpose, 5 specifically for construction purpose, 10 for ornamental and 13 species reported to have miscellaneous uses. MCA accounts to 9 flowering plant species for research and development and agro-technology development and 12 species are listed under IUCN and CAMP threatened categories. MCA and its affinity are likewise house to 10 endemic flowering plants. As from the study it can be inferred that Manaslu Conservation Area (MCA) is a treasure hub of flowering plants that not only represent its economic importance rather it is also a house to threaten and endemic flowering plants. Thus, proper management plans must be implemented for the conservation of resources in MCA.