Objective To study the relevance of expression of basic fibroblast growth factor (bFGF), fibroblast growth factor receptor 1 (FGFR 1) and carcinogenesis and progression of ovarian epithelial neoplasm. Methods Ten ...Objective To study the relevance of expression of basic fibroblast growth factor (bFGF), fibroblast growth factor receptor 1 (FGFR 1) and carcinogenesis and progression of ovarian epithelial neoplasm. Methods Ten cases of normal ovarian tissues and 75 cases of ovarian epithelial neoplasm tissues were detected by immunohistochemical methods: S P for bFGF, FGFR 1,double immunohistochemistry Lab SA for Ki 67 antigen and bFGF. Results The expression level of bFGF, FGFR 1in ovarian epithelium and ovarian epithelial neoplasm showed a step wise increase in the following order:normal <benign <borderline <malignant; The expression level and intensity of bFGF and FGFR 1 were increased with the decrease of differentiation degree and increase of clinical stage in ovarian carcinoma; There was no statistical difference between the expression of bFGF, FGFR 1 in serous cystadenocarcinoma and that of mucinous cystadenocarcinoma; The expression of bFGF was correlated with that of FGFR 1 in neoplastic tissues; There were positive expression rates of bFGF and Ki 67 antigen in ovarian epithelial neoplasm. Conclusion As an important proliferative factor, bFGF plays an important role in carcinogenisis and progression of ovarian epithelial neoplasm.展开更多
目的探讨利用一代测序技术检测成纤维细胞生长因子受体(F G F R 3)进行软骨发育不全(achondroplasis,ACH)的产前诊断的价值。方法利用聚合酶链反应(polymerase chain reaction,PCR)技术扩增FGFR3基因10号外显子,利用一代测序法对其进行...目的探讨利用一代测序技术检测成纤维细胞生长因子受体(F G F R 3)进行软骨发育不全(achondroplasis,ACH)的产前诊断的价值。方法利用聚合酶链反应(polymerase chain reaction,PCR)技术扩增FGFR3基因10号外显子,利用一代测序法对其进行分析,对2个临床确诊为ACH的患者以及3个ACH家系和3个产前三维B超显示胎儿骨骼发育异常的家系进行基因检测和产前诊断。结果两例临床确诊为软骨发育不全的患者为c.1183G>A的热点突变,6个产前诊断家系中,3个家系结果为阳性,3个家系结果为阴性。结论对FGFR3基因第10号外显子进行检测可以为ACH患者以及怀有疑似ACH胎儿的家庭提供诊断依据。展开更多
文摘Objective To study the relevance of expression of basic fibroblast growth factor (bFGF), fibroblast growth factor receptor 1 (FGFR 1) and carcinogenesis and progression of ovarian epithelial neoplasm. Methods Ten cases of normal ovarian tissues and 75 cases of ovarian epithelial neoplasm tissues were detected by immunohistochemical methods: S P for bFGF, FGFR 1,double immunohistochemistry Lab SA for Ki 67 antigen and bFGF. Results The expression level of bFGF, FGFR 1in ovarian epithelium and ovarian epithelial neoplasm showed a step wise increase in the following order:normal <benign <borderline <malignant; The expression level and intensity of bFGF and FGFR 1 were increased with the decrease of differentiation degree and increase of clinical stage in ovarian carcinoma; There was no statistical difference between the expression of bFGF, FGFR 1 in serous cystadenocarcinoma and that of mucinous cystadenocarcinoma; The expression of bFGF was correlated with that of FGFR 1 in neoplastic tissues; There were positive expression rates of bFGF and Ki 67 antigen in ovarian epithelial neoplasm. Conclusion As an important proliferative factor, bFGF plays an important role in carcinogenisis and progression of ovarian epithelial neoplasm.
文摘目的探讨利用一代测序技术检测成纤维细胞生长因子受体(F G F R 3)进行软骨发育不全(achondroplasis,ACH)的产前诊断的价值。方法利用聚合酶链反应(polymerase chain reaction,PCR)技术扩增FGFR3基因10号外显子,利用一代测序法对其进行分析,对2个临床确诊为ACH的患者以及3个ACH家系和3个产前三维B超显示胎儿骨骼发育异常的家系进行基因检测和产前诊断。结果两例临床确诊为软骨发育不全的患者为c.1183G>A的热点突变,6个产前诊断家系中,3个家系结果为阳性,3个家系结果为阴性。结论对FGFR3基因第10号外显子进行检测可以为ACH患者以及怀有疑似ACH胎儿的家庭提供诊断依据。
