Objective：To observe the effect and mechanism of Chinese medicine therapy for activating blood and dredging collaterals（ABDC） on treating systemic lupus erythematosus complicated with avascular necrosis of the femo...Objective：To observe the effect and mechanism of Chinese medicine therapy for activating blood and dredging collaterals（ABDC） on treating systemic lupus erythematosus complicated with avascular necrosis of the femoral head（SLE-ANFH）.Methods：Thirty-four patients（51 joints） with SLE-ANFH were assigned by a random number table to two groups：22 patients（32 joints） in the treatment group and 12 patients （19 joints） in the control group.All received Western medical conventional treatment for anti-inflammation and immunosuppression,but an additional Chinese medicine decoction prescribed based on ABDC principle was administered to patients in the treatment group.The observation on the patients＇ condition and therapeutic effect lasted for 3 years.Results：The patients＇ conditions in the two groups,as assessed by Association for Research Circulation Osseous（ARCO） staging,were similar before treatment.After treatment,comparison between groups showed significant difference（P0.05）,and the raised Harris functional scores in the treatment group were higher than that in the control group（P0.01）.The post-treatment symptom improving rate in the treated group was 72.73%,which was higher than that in the control group（50.00%,P0.05）.Moreover,the former was superior in improving hematologic and hemorrheologic parameters in terms of prolonging activated partial thromboplastin time,lowering whole blood middle/low shear viscosity,and plasma viscosity（P0.05 or P0.01）. Two patients in the control group but none in the treatment group received hip joint replacement operation during the observation period.Conclusions：Chinese medicine ABDC therapy could effectively alleviate clinical symptoms and improve joint function of patients with SLE-ANFH.The mechanism may be related to its effects on improving high coagulation manner and trend for getting embolism.展开更多
目的探索一种治疗股骨头早期缺血性坏死(avascu lar necros is of fem ora l head,AVNFH)的新方法。方法69只AVNFH造模成功后的新西兰大白兔,随机分成3组。A组,脱蛋白骨(deprote in ized bone,DPB)复合pcDNA 3.1/血管内皮细胞生长因子16...目的探索一种治疗股骨头早期缺血性坏死(avascu lar necros is of fem ora l head,AVNFH)的新方法。方法69只AVNFH造模成功后的新西兰大白兔,随机分成3组。A组,脱蛋白骨(deprote in ized bone,DPB)复合pcDNA 3.1/血管内皮细胞生长因子165(vascu lar endothe lia l grow th factor 165,VEGF 165)质粒植入坏死的股骨头内;B组植入DPB;C组仅在股骨头内钻一隧道。术后3 d,1、2、4、8和16周切取股骨头标本。用RT-PCR检测VEGF 165mRNA的表达;W estern b lot和免疫组织化学技术检测VEGF 165蛋白的表达;X线片观察成骨情况;组织形态学分析血管发生和新骨形成情况。结果A组术后3 d即有VEGF 165 mRNA的表达,术后1周达高峰,表达时间超过3周;术后2、4和8周Ⅰ型胶原的面积积分光密度值分别为0.29±0.11、0.55±0.13和0.67±0.10 IOD/μm2,与B、C组比较差异有统计学意义(P<0.01);X线片示A组骨痂形成早且多,B组术后4、8周骨痂少于A组,C组骨痂生成不明显;术后2周和4周A组血管面积积分光密度值分别为0.33±0.10和0.57±0.16 IOD/μm2,与B、C组比较差异有统计学意义(P<0.01)。结论VEGF 165基因转染可促进局部血管的早期形成,DPB-VEGF 165复合物可增加骨形成。DPB联合VEGF 165基因治疗为骨坏死的修复提供了理论基础。展开更多
文摘Objective：To observe the effect and mechanism of Chinese medicine therapy for activating blood and dredging collaterals（ABDC） on treating systemic lupus erythematosus complicated with avascular necrosis of the femoral head（SLE-ANFH）.Methods：Thirty-four patients（51 joints） with SLE-ANFH were assigned by a random number table to two groups：22 patients（32 joints） in the treatment group and 12 patients （19 joints） in the control group.All received Western medical conventional treatment for anti-inflammation and immunosuppression,but an additional Chinese medicine decoction prescribed based on ABDC principle was administered to patients in the treatment group.The observation on the patients＇ condition and therapeutic effect lasted for 3 years.Results：The patients＇ conditions in the two groups,as assessed by Association for Research Circulation Osseous（ARCO） staging,were similar before treatment.After treatment,comparison between groups showed significant difference（P0.05）,and the raised Harris functional scores in the treatment group were higher than that in the control group（P0.01）.The post-treatment symptom improving rate in the treated group was 72.73%,which was higher than that in the control group（50.00%,P0.05）.Moreover,the former was superior in improving hematologic and hemorrheologic parameters in terms of prolonging activated partial thromboplastin time,lowering whole blood middle/low shear viscosity,and plasma viscosity（P0.05 or P0.01）. Two patients in the control group but none in the treatment group received hip joint replacement operation during the observation period.Conclusions：Chinese medicine ABDC therapy could effectively alleviate clinical symptoms and improve joint function of patients with SLE-ANFH.The mechanism may be related to its effects on improving high coagulation manner and trend for getting embolism.
文摘目的探索一种治疗股骨头早期缺血性坏死(avascu lar necros is of fem ora l head,AVNFH)的新方法。方法69只AVNFH造模成功后的新西兰大白兔,随机分成3组。A组,脱蛋白骨(deprote in ized bone,DPB)复合pcDNA 3.1/血管内皮细胞生长因子165(vascu lar endothe lia l grow th factor 165,VEGF 165)质粒植入坏死的股骨头内;B组植入DPB;C组仅在股骨头内钻一隧道。术后3 d,1、2、4、8和16周切取股骨头标本。用RT-PCR检测VEGF 165mRNA的表达;W estern b lot和免疫组织化学技术检测VEGF 165蛋白的表达;X线片观察成骨情况;组织形态学分析血管发生和新骨形成情况。结果A组术后3 d即有VEGF 165 mRNA的表达,术后1周达高峰,表达时间超过3周;术后2、4和8周Ⅰ型胶原的面积积分光密度值分别为0.29±0.11、0.55±0.13和0.67±0.10 IOD/μm2,与B、C组比较差异有统计学意义(P<0.01);X线片示A组骨痂形成早且多,B组术后4、8周骨痂少于A组,C组骨痂生成不明显;术后2周和4周A组血管面积积分光密度值分别为0.33±0.10和0.57±0.16 IOD/μm2,与B、C组比较差异有统计学意义(P<0.01)。结论VEGF 165基因转染可促进局部血管的早期形成,DPB-VEGF 165复合物可增加骨形成。DPB联合VEGF 165基因治疗为骨坏死的修复提供了理论基础。
