The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrin...The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrinia furnacalis.Results showed that the expression peaks of npfmRNA in both midgut and fat body are at 28 h of early 5th instar larvae.When NPF was kno eked dow n either by feedi ng dsNPF-c ontaining artificial diet or direct dsNPF in jection into the 5th in star larvae,npf expression was effectively inhibited in the midgut.Larval feeding,body weight and development time were significantly impacted.In con trast,the expressi on of the npf receptors npfrl and npfr2 in fat body of 5th in star larvae was sign ifica ntly increased by dsNPF injection.These results indicate that NPF can be effectively knoeked down in 0.furnacalis,and dsNPF by injection is an effective and fast way to silence npf expression.This study provides a critical basis for further exploration in mechanism of feeding regulation in 0.furnacalis.展开更多
Genetic modification (GM) represents new opportunities for enhanced crop features such as improved insect resistance and herbicide tolerance. The technology allows for cross-species alterations, therefore potentially ...Genetic modification (GM) represents new opportunities for enhanced crop features such as improved insect resistance and herbicide tolerance. The technology allows for cross-species alterations, therefore potentially allowing a vast array of novel traits. Many GM crops have been developed and approved for human and animal consumption. The present study investigated a triple-stacked GM corn variety containing modifications for insect resistance (via cry1Ab and cry3Bb1 genes) and herbicide tolerance (via an EPSPS gene), which was fed to rats for six months. The study investigated the mucosa of the stomach. Alterations to tight junction apposition, gland dilatations with epithelial elongation and dysplasia in the GM-fed rats were observed. These results indicate that GM-corn may have an effect on rat stomach mucosa, which may have health implications.展开更多
基金This work is supported by the National Natural Science Foundation of China(31730076).
文摘The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrinia furnacalis.Results showed that the expression peaks of npfmRNA in both midgut and fat body are at 28 h of early 5th instar larvae.When NPF was kno eked dow n either by feedi ng dsNPF-c ontaining artificial diet or direct dsNPF in jection into the 5th in star larvae,npf expression was effectively inhibited in the midgut.Larval feeding,body weight and development time were significantly impacted.In con trast,the expressi on of the npf receptors npfrl and npfr2 in fat body of 5th in star larvae was sign ifica ntly increased by dsNPF injection.These results indicate that NPF can be effectively knoeked down in 0.furnacalis,and dsNPF by injection is an effective and fast way to silence npf expression.This study provides a critical basis for further exploration in mechanism of feeding regulation in 0.furnacalis.
文摘Genetic modification (GM) represents new opportunities for enhanced crop features such as improved insect resistance and herbicide tolerance. The technology allows for cross-species alterations, therefore potentially allowing a vast array of novel traits. Many GM crops have been developed and approved for human and animal consumption. The present study investigated a triple-stacked GM corn variety containing modifications for insect resistance (via cry1Ab and cry3Bb1 genes) and herbicide tolerance (via an EPSPS gene), which was fed to rats for six months. The study investigated the mucosa of the stomach. Alterations to tight junction apposition, gland dilatations with epithelial elongation and dysplasia in the GM-fed rats were observed. These results indicate that GM-corn may have an effect on rat stomach mucosa, which may have health implications.
