The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize ...The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM （genetically modified） products. Three anti-herbicide genes （CP4- EPSPS, BAR and PAT） are common exogenous genes used in commercialized transgenic soybean, maize and rice, In the present study, a new SYBR Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products （soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam） using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.展开更多
通过对以问题为基础的学习(problem based leaning,PBL)的教学模式分析理解,进行了基于PBL和"教学做"三位一体的《饲料分析检测学》课程教学模式实践。阐述了课程教学实践过程、课程教学效果评价体系和具体教学方法,旨在为&qu...通过对以问题为基础的学习(problem based leaning,PBL)的教学模式分析理解,进行了基于PBL和"教学做"三位一体的《饲料分析检测学》课程教学模式实践。阐述了课程教学实践过程、课程教学效果评价体系和具体教学方法,旨在为"动物营养与饲料课程体系教学质量工程"的建设提供实践基础。展开更多
基金Supported by the Funds of the Scientific Research Foundation of Northeast Agricultural University(2010RCB53)
文摘The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM （genetically modified） products. Three anti-herbicide genes （CP4- EPSPS, BAR and PAT） are common exogenous genes used in commercialized transgenic soybean, maize and rice, In the present study, a new SYBR Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products （soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam） using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.
