背景与目的:EB病毒潜伏膜蛋白1(latent membrane protein 1,LMP1)是一重要的癌基因。Fascin蛋白是一种能与F-肌动蛋白结合的细胞骨架蛋白,参与细胞的迁移、粘附等功能。磷酸化的信号转导及转录激活因子3(phosphorylated signal transduc...背景与目的:EB病毒潜伏膜蛋白1(latent membrane protein 1,LMP1)是一重要的癌基因。Fascin蛋白是一种能与F-肌动蛋白结合的细胞骨架蛋白,参与细胞的迁移、粘附等功能。磷酸化的信号转导及转录激活因子3(phosphorylated signal transducer and activator of transcription 3,pStat3)是信号转导及转录激活因子(STATs)家族中与人类肿瘤发生发展密切相关的一种蛋白。本实验通过检测鼻咽癌及淋巴结转移癌中LMP1、Fascin和pStat3的表达,以探讨这些指标在鼻咽癌发生发展中的意义。方法:采用原位杂交和免疫组化法分别检测43例鼻咽癌组织(21例伴淋巴结转移;22例未伴淋巴结转移)及相应的21例癌转移淋巴结中EB病毒编码的非多聚腺苷酸RNA(EBV-encoded small RNA,EBER)的表达及LMP1、Fascin、pStat3、p53、Ki-67和Bcl-2的表达,并采用统计学分析上述各蛋白表达水平之间的相关性。采用Western blot检测转染LMP1表达质粒对鼻咽癌细胞中Fascin和pStat3表达的影响。结果:43例鼻咽癌组织及21例癌转移淋巴结中,EBER均呈阳性表达;43例鼻咽癌组织中,LMP1、Fascin、pStat3、p53、Ki-67、Bcl-2的阳性表达率分别为69.8%(30/43)、93.0%(40/43)、72.1%(31/43)、90.7%(39/43)、88.4%(38/43)、88.4%(38/43),LMP1的表达与其余5种指标的表达之间均具有明显的正相关(P<0.05)。21例鼻咽癌淋巴结转移组织中LMP1表达率为46.7%(10/21)。LMP1表达可明显提高鼻咽癌细胞中Fascin和pStat3的表达水平。结论:鼻咽癌组织及淋巴结转移癌组织中LMP1均有较高的表达率。鼻咽癌组织中LMP1表达与Fascin和pStat3表达之间均存在正相关。LMP1表达可明显上调鼻咽癌细胞中Fascin和pStat3的表达水平。LMP1的表达与癌细胞的增殖指数密切相关。展开更多
AIM:To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer. METHODS:The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa...AIM:To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer. METHODS:The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa-2. The proliferation, cell cycle, motility, scattering, invasiveness and organization of the actin filament system in fascin-transfected MIA PaCa-2 cells and control non-transfected cells were determined. RESULTS:Heterogeneous overexpression of fascin markedly enhanced the motility, scattering, and inva-siveness of MIA PaCa-2 cells. However, overexpression of fascin had minimal effect on MIA PaCa-2 cell pro-liferation and cell cycle. In addition, cell morphology and organization of the actin filament system were distinctly altered in fascin overexpressed cells. When transplanted into BALB/c-nu mice, fascin-transfected pancreatic cancer cells developed solid tumors at a slightly slower rate, but these tumors displayed more aggressive behavior in comparison with control tumors. CONCLUSION: Fascin promotes pancreatic cancer cell migration, invasion and scattering, thus contributes to the aggressive behavior of pancreatic cancer cells.展开更多
文摘背景与目的:EB病毒潜伏膜蛋白1(latent membrane protein 1,LMP1)是一重要的癌基因。Fascin蛋白是一种能与F-肌动蛋白结合的细胞骨架蛋白,参与细胞的迁移、粘附等功能。磷酸化的信号转导及转录激活因子3(phosphorylated signal transducer and activator of transcription 3,pStat3)是信号转导及转录激活因子(STATs)家族中与人类肿瘤发生发展密切相关的一种蛋白。本实验通过检测鼻咽癌及淋巴结转移癌中LMP1、Fascin和pStat3的表达,以探讨这些指标在鼻咽癌发生发展中的意义。方法:采用原位杂交和免疫组化法分别检测43例鼻咽癌组织(21例伴淋巴结转移;22例未伴淋巴结转移)及相应的21例癌转移淋巴结中EB病毒编码的非多聚腺苷酸RNA(EBV-encoded small RNA,EBER)的表达及LMP1、Fascin、pStat3、p53、Ki-67和Bcl-2的表达,并采用统计学分析上述各蛋白表达水平之间的相关性。采用Western blot检测转染LMP1表达质粒对鼻咽癌细胞中Fascin和pStat3表达的影响。结果:43例鼻咽癌组织及21例癌转移淋巴结中,EBER均呈阳性表达;43例鼻咽癌组织中,LMP1、Fascin、pStat3、p53、Ki-67、Bcl-2的阳性表达率分别为69.8%(30/43)、93.0%(40/43)、72.1%(31/43)、90.7%(39/43)、88.4%(38/43)、88.4%(38/43),LMP1的表达与其余5种指标的表达之间均具有明显的正相关(P<0.05)。21例鼻咽癌淋巴结转移组织中LMP1表达率为46.7%(10/21)。LMP1表达可明显提高鼻咽癌细胞中Fascin和pStat3的表达水平。结论:鼻咽癌组织及淋巴结转移癌组织中LMP1均有较高的表达率。鼻咽癌组织中LMP1表达与Fascin和pStat3表达之间均存在正相关。LMP1表达可明显上调鼻咽癌细胞中Fascin和pStat3的表达水平。LMP1的表达与癌细胞的增殖指数密切相关。
基金Chen J designed and supervised the research and gave funding supportSupported by Grants from the Doctoral Fund from the Ministry of Education of China,No.20060023013+4 种基金the National Nature Science Foundation of China,No.30471970 and 30973470the National Science and Technology Support Project(the1 1th Five-Year Plan)of China,No.2006BAI02A14the Scientific Research Special Projects of Health Ministry of China,No.200802011the National Data Sharing Project in Human Health,No.2005DKA32403Roche Company
文摘AIM:To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer. METHODS:The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa-2. The proliferation, cell cycle, motility, scattering, invasiveness and organization of the actin filament system in fascin-transfected MIA PaCa-2 cells and control non-transfected cells were determined. RESULTS:Heterogeneous overexpression of fascin markedly enhanced the motility, scattering, and inva-siveness of MIA PaCa-2 cells. However, overexpression of fascin had minimal effect on MIA PaCa-2 cell pro-liferation and cell cycle. In addition, cell morphology and organization of the actin filament system were distinctly altered in fascin overexpressed cells. When transplanted into BALB/c-nu mice, fascin-transfected pancreatic cancer cells developed solid tumors at a slightly slower rate, but these tumors displayed more aggressive behavior in comparison with control tumors. CONCLUSION: Fascin promotes pancreatic cancer cell migration, invasion and scattering, thus contributes to the aggressive behavior of pancreatic cancer cells.
