OBJECTIVE Cloves(Syzygium aromaticumL.) have been used as both a spice and a traditional Chinese medicinal herb for thousands of years.However,relatively little is known about its potential anticancer activity and mec...OBJECTIVE Cloves(Syzygium aromaticumL.) have been used as both a spice and a traditional Chinese medicinal herb for thousands of years.However,relatively little is known about its potential anticancer activity and mechanisms.In this study,we investigated the in vitro and in vivo anti.tumor effects and mechanisms of water extract of cloves(WEC) against colorectal cancer.METHODS MTS assay and Colony-formation assay were used to detect the anti-tumor activity of WEC on HT-29 cells.The in vivo anti-tumor effect of WEC was detected in a subcutaneous transplantation tumor model of human HT-29 cells.Autophagy was detected by flow cytometry and the expressions of autophagy related proteins(Beclin-1 and LC-3 a/b) were determined by western blot.RESULTS MTS result showed that WEC significantly inhibited the viability of HT-29 cells,with the IC50 values of 150 μg·mL-1.The colonyformation assay showed that the WEC significantly suppressed colon cancer cells proliferation.WEC also exhibited significant antitumor activity in tumor bearing nude mice.Flow cytometry result showed that WEC significantly induced autophagy,and the averaged relative values of fluorescence intensity were206,251,341 and 356 in cells treated with 0,100,150 and 200 μg·mL-1 WEC for 48 h.Western blot result showed that WEC treatment significantly increased Beclin-1 expression and ratios of LC3-II/LC3-I.CONCLUSION These result showed that WEC inhibited the growth of colon tumor both in vitro and in vivo,which might be related with autophagy induction,and WEC has potential to be developed as a novel anticancer agent for the treatment of colon cancer.展开更多
文摘OBJECTIVE Cloves(Syzygium aromaticumL.) have been used as both a spice and a traditional Chinese medicinal herb for thousands of years.However,relatively little is known about its potential anticancer activity and mechanisms.In this study,we investigated the in vitro and in vivo anti.tumor effects and mechanisms of water extract of cloves(WEC) against colorectal cancer.METHODS MTS assay and Colony-formation assay were used to detect the anti-tumor activity of WEC on HT-29 cells.The in vivo anti-tumor effect of WEC was detected in a subcutaneous transplantation tumor model of human HT-29 cells.Autophagy was detected by flow cytometry and the expressions of autophagy related proteins(Beclin-1 and LC-3 a/b) were determined by western blot.RESULTS MTS result showed that WEC significantly inhibited the viability of HT-29 cells,with the IC50 values of 150 μg·mL-1.The colonyformation assay showed that the WEC significantly suppressed colon cancer cells proliferation.WEC also exhibited significant antitumor activity in tumor bearing nude mice.Flow cytometry result showed that WEC significantly induced autophagy,and the averaged relative values of fluorescence intensity were206,251,341 and 356 in cells treated with 0,100,150 and 200 μg·mL-1 WEC for 48 h.Western blot result showed that WEC treatment significantly increased Beclin-1 expression and ratios of LC3-II/LC3-I.CONCLUSION These result showed that WEC inhibited the growth of colon tumor both in vitro and in vivo,which might be related with autophagy induction,and WEC has potential to be developed as a novel anticancer agent for the treatment of colon cancer.
