<abstract>Aim: To study the effect of experimental left varicocele (ELV) on epididymal structure and function in adolescent Sprague-Dawley rats. Methods: ELV was induced by partial ligation of the left renal vei...<abstract>Aim: To study the effect of experimental left varicocele (ELV) on epididymal structure and function in adolescent Sprague-Dawley rats. Methods: ELV was induced by partial ligation of the left renal vein. Sham-operated animals served as the controls. Four and 8 weeks after the operation, the histological, ultrastructural and biochemical (alpha-glucosidase activity and carnitine content) changes in different segments of the epididymis were observed. Results: In the treated animals, there were degeneration of the epididymal epithelium and edema of the interstitial tissue; numerous shedding cells, residual bodies, deformed sperm and macrophages appeared in the epididymal lumen. Morphometric measurement indicated a significant reduction in the epididymal tubular diameter (P<0.05) and a significant increase in the epididymal interstitial area (P<0.05) compared with the controls. Ultrastructural study showed sparse microvilli of the columnar epithelium, increased and enlarged lysosomes in the principal cells with defected organelles and the presence of large cytoplasmic vacuoles. The protein and carnitine contents and the alpha-glucosidase activity in the caput, corpus and cauda epididymis of the ELV rats were lower than those of the controls (P< 0.05). Conclusion: There were structural and functional changes in the epididymis of adolescent ELV rats, which may contribute to the infertility caused by varicocele.展开更多
目的研究VEGF及KDR在实验性左侧精索静脉曲张4周组及8周组大鼠睾丸中的表达变化,探讨它们与精索静脉曲张(VC)的关系及VC致男性不育的病理生理学机制。方法建立大鼠实验性左侧精索静脉曲张模型,于术后4周和8周取双侧睾丸,采用免疫组织化...目的研究VEGF及KDR在实验性左侧精索静脉曲张4周组及8周组大鼠睾丸中的表达变化,探讨它们与精索静脉曲张(VC)的关系及VC致男性不育的病理生理学机制。方法建立大鼠实验性左侧精索静脉曲张模型,于术后4周和8周取双侧睾丸,采用免疫组织化学SP法检测VEGF、KDR在实验组和对照组SD大鼠睾丸上的表达情况。结果 ELV 4周组大鼠两侧睾丸中VEGF蛋白表达均上调,8周组表达均下降;ELV4周组大鼠睾丸中KDR蛋白的表达与对照组比较显著增强,而8周组比对照组和4周组均显著降低。结论 VEGF和KDR在睾丸中的表达明显受到ELV进程的影响,说明它们在VC致男性不育中起到一定的作用。展开更多
文摘<abstract>Aim: To study the effect of experimental left varicocele (ELV) on epididymal structure and function in adolescent Sprague-Dawley rats. Methods: ELV was induced by partial ligation of the left renal vein. Sham-operated animals served as the controls. Four and 8 weeks after the operation, the histological, ultrastructural and biochemical (alpha-glucosidase activity and carnitine content) changes in different segments of the epididymis were observed. Results: In the treated animals, there were degeneration of the epididymal epithelium and edema of the interstitial tissue; numerous shedding cells, residual bodies, deformed sperm and macrophages appeared in the epididymal lumen. Morphometric measurement indicated a significant reduction in the epididymal tubular diameter (P<0.05) and a significant increase in the epididymal interstitial area (P<0.05) compared with the controls. Ultrastructural study showed sparse microvilli of the columnar epithelium, increased and enlarged lysosomes in the principal cells with defected organelles and the presence of large cytoplasmic vacuoles. The protein and carnitine contents and the alpha-glucosidase activity in the caput, corpus and cauda epididymis of the ELV rats were lower than those of the controls (P< 0.05). Conclusion: There were structural and functional changes in the epididymis of adolescent ELV rats, which may contribute to the infertility caused by varicocele.
文摘目的研究VEGF及KDR在实验性左侧精索静脉曲张4周组及8周组大鼠睾丸中的表达变化,探讨它们与精索静脉曲张(VC)的关系及VC致男性不育的病理生理学机制。方法建立大鼠实验性左侧精索静脉曲张模型,于术后4周和8周取双侧睾丸,采用免疫组织化学SP法检测VEGF、KDR在实验组和对照组SD大鼠睾丸上的表达情况。结果 ELV 4周组大鼠两侧睾丸中VEGF蛋白表达均上调,8周组表达均下降;ELV4周组大鼠睾丸中KDR蛋白的表达与对照组比较显著增强,而8周组比对照组和4周组均显著降低。结论 VEGF和KDR在睾丸中的表达明显受到ELV进程的影响,说明它们在VC致男性不育中起到一定的作用。
