Sinapine derived from cruciferous plants could be converted into trimethylamine by intestinal microbiota.Its metabolite,trimethylamine N-oxide(TMAO),is closely linked to increased risk of cardiovascular disease and fa...Sinapine derived from cruciferous plants could be converted into trimethylamine by intestinal microbiota.Its metabolite,trimethylamine N-oxide(TMAO),is closely linked to increased risk of cardiovascular disease and fat deposition in mammals.Hens fed with rapeseed meal(RSM)suffered from fatty liver hemorrhage syndrome(FLHS).This study was conducted to investigate whether RSM-induced fatty liver is due to TMAO via altering microbiota composition and diversity.At 33 weeks of age,600 laying hens were randomly divided into 5 treatment groups,namely control and 14%RSM treatment groups(DY5,with 16.2% erucic acid [EA]and 74.66% glucosinolate[Gl]contents;MB1,with 3.50% EA and 43.23 %Gl contents;DY6,with 6.7% EA and 22.67% Gl contents;XH3,with 44.60% EA and 132.83% Gl contents)for 8 weeks.Results revealed that 3 hens died due to liver hemorrhage after ingesting 14% RSM diet.The 14% RSM decreased serum low-density lipoprotein cholesterol(LDL-C)content(P<0.01)while tended to increase serum TMAO content compared to the control group(P=0.08).The 14% RSM diet increased red oil O optical density(P<0.01),and increased total cholesterol(TC)and LDL-C content in the liver(P<0.01,and P<0.01,respectively).The 14%RSM decreased liver total bile acid(TBA)content compared to the control(P<0.01).The DY6 had a higher TBA content in the liver than the XH3(P<0.01).The 14%RSM decreased mRNA abundance of liver X receptors alpha(LXR-α,P=0.01),and increased mRNA abundance of sterol response element binding protein 2(SREBP-2,P=0.04).Results revealed that the in-feed RSM could alter richness and diversity of cecal microbiota compared to the control(P<0.05).Liver TC content and serum TMAO showed a negative relationship with Proteobacteria and Actinobacteria(P=0.04).In conclusion,14%RSM increased liver TC and induced high liver score of FLHS,which was possibly associated with the altered cecal microbiota composition,increased serum TMAO levels and LXR-αand SREBP-2 expressions.展开更多
The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain(ADG),concentrate intakes,incidence of diarrhea,serum haptoglobin,interleukin(IL)-1,and resolvin-E1 in f...The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain(ADG),concentrate intakes,incidence of diarrhea,serum haptoglobin,interleukin(IL)-1,and resolvin-E1 in female Holstein calves from birth to weaning.A sample size of 20 calves per group was calculated and were randomly allocated at the time of birth.Linseed group(LIN)was fed a starter with linseed expeller,while canola group(CAN)received a similar concentrate,but with canola expeller.Both expellers were included at a rate of 25%dry matter(DM)basis of the starter.Pasteurized waste milk was fed twice a day until weaning.Calves were weighed at birth,30,and 60 d of age.Starter intake was evaluated daily from 5 to 60 d.A blood sample was obtained at birth,14,28,35,and 49 d of age,and bovine serum resolvin-El,haptoglobin,and IL-1 were assayed by commercial enzyme-linked immunosorbent assay(ELISA)kits.Incidence of diarrhea and the duration of the events were also recorded.The effect of the interaction group by time on body weight(BW)and starter intake was not significant(P>0.05).Average daily gains(ADG)from 0 to 60 d for CAN and LIN groups were 0.680 and 0.675 kg/d(P>0.05),respectively.Incidences of diarrhea were 25%and 45%for CAN and LIN groups,respectively(P=0.18).LIN group had greater concentrations of IL-1 at d 21,haptoglobin at d 7,and resolving-El at d 14 and 49 than CAN group,respectively.It is concluded that BW at weaning,ADG,and concentrate intakes were not different between groups fed starters containing linseed or canola expeller(25%inclusion).The concentrations of cytokines and haptoglobin were the greatest in LIN group.展开更多
基金fanatically supported by National Science and Technology Project(2014BAD13B04)the Sichuan Provincial Science and Technology project(2018NZ20009,2014NZ0043)。
文摘Sinapine derived from cruciferous plants could be converted into trimethylamine by intestinal microbiota.Its metabolite,trimethylamine N-oxide(TMAO),is closely linked to increased risk of cardiovascular disease and fat deposition in mammals.Hens fed with rapeseed meal(RSM)suffered from fatty liver hemorrhage syndrome(FLHS).This study was conducted to investigate whether RSM-induced fatty liver is due to TMAO via altering microbiota composition and diversity.At 33 weeks of age,600 laying hens were randomly divided into 5 treatment groups,namely control and 14%RSM treatment groups(DY5,with 16.2% erucic acid [EA]and 74.66% glucosinolate[Gl]contents;MB1,with 3.50% EA and 43.23 %Gl contents;DY6,with 6.7% EA and 22.67% Gl contents;XH3,with 44.60% EA and 132.83% Gl contents)for 8 weeks.Results revealed that 3 hens died due to liver hemorrhage after ingesting 14% RSM diet.The 14% RSM decreased serum low-density lipoprotein cholesterol(LDL-C)content(P<0.01)while tended to increase serum TMAO content compared to the control group(P=0.08).The 14% RSM diet increased red oil O optical density(P<0.01),and increased total cholesterol(TC)and LDL-C content in the liver(P<0.01,and P<0.01,respectively).The 14%RSM decreased liver total bile acid(TBA)content compared to the control(P<0.01).The DY6 had a higher TBA content in the liver than the XH3(P<0.01).The 14%RSM decreased mRNA abundance of liver X receptors alpha(LXR-α,P=0.01),and increased mRNA abundance of sterol response element binding protein 2(SREBP-2,P=0.04).Results revealed that the in-feed RSM could alter richness and diversity of cecal microbiota compared to the control(P<0.05).Liver TC content and serum TMAO showed a negative relationship with Proteobacteria and Actinobacteria(P=0.04).In conclusion,14%RSM increased liver TC and induced high liver score of FLHS,which was possibly associated with the altered cecal microbiota composition,increased serum TMAO levels and LXR-αand SREBP-2 expressions.
文摘The objective of this study was to compare the effect of linseed and canola expeller on average daily weight gain(ADG),concentrate intakes,incidence of diarrhea,serum haptoglobin,interleukin(IL)-1,and resolvin-E1 in female Holstein calves from birth to weaning.A sample size of 20 calves per group was calculated and were randomly allocated at the time of birth.Linseed group(LIN)was fed a starter with linseed expeller,while canola group(CAN)received a similar concentrate,but with canola expeller.Both expellers were included at a rate of 25%dry matter(DM)basis of the starter.Pasteurized waste milk was fed twice a day until weaning.Calves were weighed at birth,30,and 60 d of age.Starter intake was evaluated daily from 5 to 60 d.A blood sample was obtained at birth,14,28,35,and 49 d of age,and bovine serum resolvin-El,haptoglobin,and IL-1 were assayed by commercial enzyme-linked immunosorbent assay(ELISA)kits.Incidence of diarrhea and the duration of the events were also recorded.The effect of the interaction group by time on body weight(BW)and starter intake was not significant(P>0.05).Average daily gains(ADG)from 0 to 60 d for CAN and LIN groups were 0.680 and 0.675 kg/d(P>0.05),respectively.Incidences of diarrhea were 25%and 45%for CAN and LIN groups,respectively(P=0.18).LIN group had greater concentrations of IL-1 at d 21,haptoglobin at d 7,and resolving-El at d 14 and 49 than CAN group,respectively.It is concluded that BW at weaning,ADG,and concentrate intakes were not different between groups fed starters containing linseed or canola expeller(25%inclusion).The concentrations of cytokines and haptoglobin were the greatest in LIN group.
