目的:探究游泳和下坡跑通过钙调磷酸酶(CN)/活化T细胞核因子(NFAT)途径对T2DM小鼠骨吸收代谢的影响。方法:采用6周高脂膳食和一次性注射链脲佐菌素(STZ)进行T2DM造模,成功后随机分为T2DM对照组(TC)、T2DM游泳组(TS)和T2DM下坡跑组(TD),...目的:探究游泳和下坡跑通过钙调磷酸酶(CN)/活化T细胞核因子(NFAT)途径对T2DM小鼠骨吸收代谢的影响。方法:采用6周高脂膳食和一次性注射链脲佐菌素(STZ)进行T2DM造模,成功后随机分为T2DM对照组(TC)、T2DM游泳组(TS)和T2DM下坡跑组(TD),另选C57小鼠为正常对照组(ZC)。T2DM小鼠继续高脂膳食,ZC小鼠饲以普通饲料。TS和TD小鼠分别进行8周游泳和下坡跑训练。末次训练24 h后处死小鼠并取材,应用Micro-CT、细胞原代培养、ELISA、RT-PCR及West-blotting等技术方法对骨组织形态计量学指标、OC数量、离子浓度、细胞因子m RNA和蛋白表达等进行检测。结果:TC组股骨中TRAF6、CN、Src-3、PLC、NFATc1、TRAP m RNA及胫骨中Src1和NFATc1蛋白表达上调(P<0.05),血清IP3和Ca2+浓度升高(P<0.05),BMM分化产生的OC总数量和≥10个核OC数量增多(P<0.01)。股骨远端松质骨和皮质骨骨组织形态计量学指标显著下降(P<0.05)。与TC比,TS组股骨中TRAF6、CN、PLC和TRAPm RNA及Src1蛋白表达下调,血清Ca2+浓度下降(P<0.05或P<0.01)。TD组股骨中TRAF6、CN、Src-3、PLC、NFATc1和TRAPm RNA及胫骨中Src1和NFATc1蛋白表达下调,血清IP3和Ca2+浓度下降(P<0.05)。OC总数量和≥10个核OC数量显著减少(P<0.05),松质骨和皮质骨骨组织形态计量学指标显著改善(P<0.05)。与TS比,TD组股骨中TRAF6、Src-3、PLC和TRAP m RNA表达下调及血清IP3和Ca^(2+)浓度下降(P<0.05),OC总数量(P<0.05)下降,松质骨BS/TV增加(P<0.05)。结论:T2DM小鼠骨吸收增强。下坡跑通过抑制T2DM小鼠骨中CN/NFAT途径,减少OC数量,降低骨吸收,改善骨组织形态结构,且其作用效果优于游泳。展开更多
目的:通过10周康复运动观察对中老年糖尿病人的功能性体适能的影响。方法:对31例II型糖尿病人合并有高血脂症患者进行Rikli and Jones[1]制定的功能性体适能测试方法进行测试。结果:10周康复运动对受测试者的BMI WHR及脉压几项危险因素...目的:通过10周康复运动观察对中老年糖尿病人的功能性体适能的影响。方法:对31例II型糖尿病人合并有高血脂症患者进行Rikli and Jones[1]制定的功能性体适能测试方法进行测试。结果:10周康复运动对受测试者的BMI WHR及脉压几项危险因素均有所降低;功能性体适能也相应提高。其余项目虽未达到统计学要求值,但也有一定积极效果。结论:康复运动可以做为中老年糖尿病人的治疗方法之一,且能收到良好效果。展开更多
OBJECTIVE: To systematically assess the effect of new exercise of Health Qigong(NEHQ) on fasting plasma glucose(FPG), 2 hour postprandial plasma glucose(2 hPG), Hemoglobin A1C(HbA1C), triglycerides(TG), total choleste...OBJECTIVE: To systematically assess the effect of new exercise of Health Qigong(NEHQ) on fasting plasma glucose(FPG), 2 hour postprandial plasma glucose(2 hPG), Hemoglobin A1C(HbA1C), triglycerides(TG), total cholesterol(TC), low density lipoprotein(LDL), and high density lipoprotein(HDL)in patients with type 2 diabetes mellitus(T2DM).METHODS: Data were retrieved by searching EMBASE,MEDLINE, The Cochrane Library, China Biology Medicine,China National Knowledge Infrastructure, Wanfang Data and Chongqing VIP Database. The random-ized controlled trials(RCTs) related to NEHQ were searched. The search terms were "fasting plasma glucose","2 hour postprandial plasma glucose","Hemoglobin A1C","TG(triglycerides)","total cholesterol","low density lipoprotein",and "high density lipoprotein". Meta-analysis were conducted with the Collaboration's Revman 5.3.RESULTS: Fourteen RCTs including 999 patients were analyzed. The levels of FPG in the patients with the intervention of NEHQ-Ba Duan Jin in 2, 6, 9 months respectively, and of NEHQ·Mawangdui Daoyin in 12 months were lower than those in the control group(P<0.05); the levels of 2hPG in the patients with the intervention of NEHQ-Ba Duan Jin in 2, 4, 6 months respectively were lower than those in the control group(P<0.05);the levels of HbAlc in the patients with the intervention of NEHQ·Ba Duan Jin in 2, 3, 4, 6, 9 months respectively, and of NEHQ·Mawangdui Daoyin 12 months were lower than those in the control group(P<0.05); the levels of TG in the patients with the intervention of NEHQ·Ba Duan Jin in 4 and 6 months respectively, and of NEHQ·Mawangdui Daoyin in 12 months were lower than those in the control group(P<0.05);the levels of TC in the patients with the intervention of NEHQ·Ba Duan Jin in 2,6, 9 months respectively were lower than those in the control group(P<0.05); the levels of LDL in the patients with the intervention of NEHQ-Ba Duan Jin in 3 and 6 months respectively were lower than those in the control group(P<0.05);the level of HDL in the patients with the展开更多
文摘目的:探究游泳和下坡跑通过钙调磷酸酶(CN)/活化T细胞核因子(NFAT)途径对T2DM小鼠骨吸收代谢的影响。方法:采用6周高脂膳食和一次性注射链脲佐菌素(STZ)进行T2DM造模,成功后随机分为T2DM对照组(TC)、T2DM游泳组(TS)和T2DM下坡跑组(TD),另选C57小鼠为正常对照组(ZC)。T2DM小鼠继续高脂膳食,ZC小鼠饲以普通饲料。TS和TD小鼠分别进行8周游泳和下坡跑训练。末次训练24 h后处死小鼠并取材,应用Micro-CT、细胞原代培养、ELISA、RT-PCR及West-blotting等技术方法对骨组织形态计量学指标、OC数量、离子浓度、细胞因子m RNA和蛋白表达等进行检测。结果:TC组股骨中TRAF6、CN、Src-3、PLC、NFATc1、TRAP m RNA及胫骨中Src1和NFATc1蛋白表达上调(P<0.05),血清IP3和Ca2+浓度升高(P<0.05),BMM分化产生的OC总数量和≥10个核OC数量增多(P<0.01)。股骨远端松质骨和皮质骨骨组织形态计量学指标显著下降(P<0.05)。与TC比,TS组股骨中TRAF6、CN、PLC和TRAPm RNA及Src1蛋白表达下调,血清Ca2+浓度下降(P<0.05或P<0.01)。TD组股骨中TRAF6、CN、Src-3、PLC、NFATc1和TRAPm RNA及胫骨中Src1和NFATc1蛋白表达下调,血清IP3和Ca2+浓度下降(P<0.05)。OC总数量和≥10个核OC数量显著减少(P<0.05),松质骨和皮质骨骨组织形态计量学指标显著改善(P<0.05)。与TS比,TD组股骨中TRAF6、Src-3、PLC和TRAP m RNA表达下调及血清IP3和Ca^(2+)浓度下降(P<0.05),OC总数量(P<0.05)下降,松质骨BS/TV增加(P<0.05)。结论:T2DM小鼠骨吸收增强。下坡跑通过抑制T2DM小鼠骨中CN/NFAT途径,减少OC数量,降低骨吸收,改善骨组织形态结构,且其作用效果优于游泳。
文摘目的:通过10周康复运动观察对中老年糖尿病人的功能性体适能的影响。方法:对31例II型糖尿病人合并有高血脂症患者进行Rikli and Jones[1]制定的功能性体适能测试方法进行测试。结果:10周康复运动对受测试者的BMI WHR及脉压几项危险因素均有所降低;功能性体适能也相应提高。其余项目虽未达到统计学要求值,但也有一定积极效果。结论:康复运动可以做为中老年糖尿病人的治疗方法之一,且能收到良好效果。
基金Research Project for Practice Development of National Traditional Chinese Medicine Clinical Research Bases(Grant No.:JDZX2012128)
文摘OBJECTIVE: To systematically assess the effect of new exercise of Health Qigong(NEHQ) on fasting plasma glucose(FPG), 2 hour postprandial plasma glucose(2 hPG), Hemoglobin A1C(HbA1C), triglycerides(TG), total cholesterol(TC), low density lipoprotein(LDL), and high density lipoprotein(HDL)in patients with type 2 diabetes mellitus(T2DM).METHODS: Data were retrieved by searching EMBASE,MEDLINE, The Cochrane Library, China Biology Medicine,China National Knowledge Infrastructure, Wanfang Data and Chongqing VIP Database. The random-ized controlled trials(RCTs) related to NEHQ were searched. The search terms were "fasting plasma glucose","2 hour postprandial plasma glucose","Hemoglobin A1C","TG(triglycerides)","total cholesterol","low density lipoprotein",and "high density lipoprotein". Meta-analysis were conducted with the Collaboration's Revman 5.3.RESULTS: Fourteen RCTs including 999 patients were analyzed. The levels of FPG in the patients with the intervention of NEHQ-Ba Duan Jin in 2, 6, 9 months respectively, and of NEHQ·Mawangdui Daoyin in 12 months were lower than those in the control group(P<0.05); the levels of 2hPG in the patients with the intervention of NEHQ-Ba Duan Jin in 2, 4, 6 months respectively were lower than those in the control group(P<0.05);the levels of HbAlc in the patients with the intervention of NEHQ·Ba Duan Jin in 2, 3, 4, 6, 9 months respectively, and of NEHQ·Mawangdui Daoyin 12 months were lower than those in the control group(P<0.05); the levels of TG in the patients with the intervention of NEHQ·Ba Duan Jin in 4 and 6 months respectively, and of NEHQ·Mawangdui Daoyin in 12 months were lower than those in the control group(P<0.05);the levels of TC in the patients with the intervention of NEHQ·Ba Duan Jin in 2,6, 9 months respectively were lower than those in the control group(P<0.05); the levels of LDL in the patients with the intervention of NEHQ-Ba Duan Jin in 3 and 6 months respectively were lower than those in the control group(P<0.05);the level of HDL in the patients with the
