Escape time algorithm is an effective theoretical algorithm of constructing fractal graphics. The key of this algorithm lies in the construction of escape time function. A new escape time function is presented based o...Escape time algorithm is an effective theoretical algorithm of constructing fractal graphics. The key of this algorithm lies in the construction of escape time function. A new escape time function is presented based on the research of escape time algorithm. An accelerated escape time algorithm is carried out in this paper. The experiments have demonstrated that the new algorithm is not only as precise as the old, but also faster when it is used to construct Julia set.展开更多
It is shown that the speed of longitudinal-extended elastic particles, emitted during an emission time T by a source S at speed u (escape speed toward the infinity due to all the masses in space), is invariant for any...It is shown that the speed of longitudinal-extended elastic particles, emitted during an emission time T by a source S at speed u (escape speed toward the infinity due to all the masses in space), is invariant for any Observer, under the Newtonian mechanics laws. It is also shown that a cosmological reason implies the light as composed of such particles moving at speed u (function of the total gravitational potential). Compliance of c with Newtonian mechanics is shown for Doppler effect, Harvard tower experiment, gravitational red shift and time dilation, highlighting, for each of these subjects, the differences versus the relativity.展开更多
Gold nanoparticles(AuNPs)assembled with fluorescent peptides through Au-S bonds(pep-AuNPs)have been widely used in biomolecular detection.However,due to the endo/lysosomal trapping after the nanoprobes enter cells,the...Gold nanoparticles(AuNPs)assembled with fluorescent peptides through Au-S bonds(pep-AuNPs)have been widely used in biomolecular detection.However,due to the endo/lysosomal trapping after the nanoprobes enter cells,the direct delivery of AuNP probes into the cytoplasm for real-time imaging remains a difficult barrier for many cytoplasm-targeting agents.Here,we prepare AuNP@gel by wrapping a multi-functional nanogel structure on the surface of a single AuNP probe by in-situ polymerization in order to directly deliver AuNP probes into the cell cytoplasm.Compared with the pep-AuNP probes,which are trapped inside lysosomes for long periods,the AuNP@gel probes use the proton-sponge effect to effectively disrupt endo/lysosomal membranes and remain in the cytoplasm.In addition,the AuNP@gel probes rapidly escape from endo/lysosomes to avoid the complex environment that interferes with the stability of the AuNP probes and the lysosomal-storage trigger the upregulation of oxidative stress into the cells.The nanogel structure enables the AuNP probes to avoid some detrimental effects and to achieve high-fidelity fluorescence signals in the cells.Compared to traditional strategies for lysosomal escape,this one-step in-situ polymerization procedure avoids the complicated modification of additional ligands and is generally applicable to peptide-,DNA-,and polymerlinked AuNP probes.展开更多
文摘Escape time algorithm is an effective theoretical algorithm of constructing fractal graphics. The key of this algorithm lies in the construction of escape time function. A new escape time function is presented based on the research of escape time algorithm. An accelerated escape time algorithm is carried out in this paper. The experiments have demonstrated that the new algorithm is not only as precise as the old, but also faster when it is used to construct Julia set.
文摘It is shown that the speed of longitudinal-extended elastic particles, emitted during an emission time T by a source S at speed u (escape speed toward the infinity due to all the masses in space), is invariant for any Observer, under the Newtonian mechanics laws. It is also shown that a cosmological reason implies the light as composed of such particles moving at speed u (function of the total gravitational potential). Compliance of c with Newtonian mechanics is shown for Doppler effect, Harvard tower experiment, gravitational red shift and time dilation, highlighting, for each of these subjects, the differences versus the relativity.
基金the National Natural Science Foundation of China(No.21775075),the Fundamental Research Funds for Central Universities(China)the Thousand Youth Talents Plan of China.
文摘Gold nanoparticles(AuNPs)assembled with fluorescent peptides through Au-S bonds(pep-AuNPs)have been widely used in biomolecular detection.However,due to the endo/lysosomal trapping after the nanoprobes enter cells,the direct delivery of AuNP probes into the cytoplasm for real-time imaging remains a difficult barrier for many cytoplasm-targeting agents.Here,we prepare AuNP@gel by wrapping a multi-functional nanogel structure on the surface of a single AuNP probe by in-situ polymerization in order to directly deliver AuNP probes into the cell cytoplasm.Compared with the pep-AuNP probes,which are trapped inside lysosomes for long periods,the AuNP@gel probes use the proton-sponge effect to effectively disrupt endo/lysosomal membranes and remain in the cytoplasm.In addition,the AuNP@gel probes rapidly escape from endo/lysosomes to avoid the complex environment that interferes with the stability of the AuNP probes and the lysosomal-storage trigger the upregulation of oxidative stress into the cells.The nanogel structure enables the AuNP probes to avoid some detrimental effects and to achieve high-fidelity fluorescence signals in the cells.Compared to traditional strategies for lysosomal escape,this one-step in-situ polymerization procedure avoids the complicated modification of additional ligands and is generally applicable to peptide-,DNA-,and polymerlinked AuNP probes.
