Utilizing Enzyme-Induced Calcium Carbonate Precipitation(EICP)reinforcement technology has emerged as an innovative approach for soil improvement.In this study,kitchen waste eggshell powder was used as an additive mat...Utilizing Enzyme-Induced Calcium Carbonate Precipitation(EICP)reinforcement technology has emerged as an innovative approach for soil improvement.In this study,kitchen waste eggshell powder was used as an additive material for EICP.The high external surface area and affinity for calcium ions of eggshell powder,which render it a suitable nucleation site for calcium carbonate precipitation.Experimental results demonstrate that the incorporation of eggshell powder,by increasing the number of nucleation sites and promoting calcium carbonate precipitation,reduces the inhibition of enzyme products,modulates the precipitation pattern of calcium carbonate,improves particle size distribution,and consequently significantly enhances the unconfined compressive strength of the samples.Furthermore,a neutral pH is achieved within the reaction system without the addition of any acid,thus preventing significant ammonia emissions.This underscores the potential of kitchen waste eggshells for recycling in biocement applications.展开更多
Human T-cell lymphophilic virus type 1(HTLV-1),the known retrovirus causing cancer in humans,is closely associated with adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis.Due ...Human T-cell lymphophilic virus type 1(HTLV-1),the known retrovirus causing cancer in humans,is closely associated with adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis.Due to its ability to evade the host's defense mechanisms,early tracking of HTLV-1 becomes crucial.In this study,we integrateλ-Exonuclease(λ-Exo)-assisted target recycling with a terminal deoxynucleotidyl transferase(TdT)-mediated template-free DNA extension process to develop an electrochemical analysis platform for the specific and sensitive detection of HTLV-1 DNA.During theλ-Exo-assisted target recycling,HTLV-1 DNA is recognized by hairpin DNA(Hp-DNA),forming double-stranded DNA(dsDNA)through DNA hybridization.The dsDNA,featuring blunt 5'terminal phosphorylation,is cleaved byλ-Exo,generating abundant short output sequence(sDNA).HTLV-1 DNA is released,initiating a cyclic hybridization-cleavage process.Subsequently,thiol-labelled capture DNA(CP-DNA)assembled on gold electrode surface captures a substantial amount of the generated sDNA,forming CP-DNA-sDNA nanostructures.When TdT and dNTPs are present on the electrode surface,the 3'-OH terminal of sDNA extends to generate long single-stranded DNA(ssDNA)structure.Methylene blue(MB)is selected as the electrochemical signal molecule.MB not only binds with ssDNA but also interacts specifically with dsDNA,resulting in a significantly enhanced electrochemical signal on modified electrode surface.The detection limit of HTLV-1 DNA is as low as 19 amol/L(S/N=3)when the two signal amplification strategies are combined.The analysis platform exhibits excellent analytical performance and holds promise as a novel tool for the early tracing and diagnosis of HTLV-1 DNA.展开更多
基金supported by JST SPRING,Grant Number JPMJSP2119supported by JSPS KAKENHI,Grant Number JP22H01581.
文摘Utilizing Enzyme-Induced Calcium Carbonate Precipitation(EICP)reinforcement technology has emerged as an innovative approach for soil improvement.In this study,kitchen waste eggshell powder was used as an additive material for EICP.The high external surface area and affinity for calcium ions of eggshell powder,which render it a suitable nucleation site for calcium carbonate precipitation.Experimental results demonstrate that the incorporation of eggshell powder,by increasing the number of nucleation sites and promoting calcium carbonate precipitation,reduces the inhibition of enzyme products,modulates the precipitation pattern of calcium carbonate,improves particle size distribution,and consequently significantly enhances the unconfined compressive strength of the samples.Furthermore,a neutral pH is achieved within the reaction system without the addition of any acid,thus preventing significant ammonia emissions.This underscores the potential of kitchen waste eggshells for recycling in biocement applications.
基金financially supported by Central Leading Local Science and Technology Development Fund Project(guikeZY22096017)Natural Science Foundation of Guangxi Province(2024GXNSFDA010036)National Natural Science Foundation of China(22164014,U23A2089).
文摘Human T-cell lymphophilic virus type 1(HTLV-1),the known retrovirus causing cancer in humans,is closely associated with adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis.Due to its ability to evade the host's defense mechanisms,early tracking of HTLV-1 becomes crucial.In this study,we integrateλ-Exonuclease(λ-Exo)-assisted target recycling with a terminal deoxynucleotidyl transferase(TdT)-mediated template-free DNA extension process to develop an electrochemical analysis platform for the specific and sensitive detection of HTLV-1 DNA.During theλ-Exo-assisted target recycling,HTLV-1 DNA is recognized by hairpin DNA(Hp-DNA),forming double-stranded DNA(dsDNA)through DNA hybridization.The dsDNA,featuring blunt 5'terminal phosphorylation,is cleaved byλ-Exo,generating abundant short output sequence(sDNA).HTLV-1 DNA is released,initiating a cyclic hybridization-cleavage process.Subsequently,thiol-labelled capture DNA(CP-DNA)assembled on gold electrode surface captures a substantial amount of the generated sDNA,forming CP-DNA-sDNA nanostructures.When TdT and dNTPs are present on the electrode surface,the 3'-OH terminal of sDNA extends to generate long single-stranded DNA(ssDNA)structure.Methylene blue(MB)is selected as the electrochemical signal molecule.MB not only binds with ssDNA but also interacts specifically with dsDNA,resulting in a significantly enhanced electrochemical signal on modified electrode surface.The detection limit of HTLV-1 DNA is as low as 19 amol/L(S/N=3)when the two signal amplification strategies are combined.The analysis platform exhibits excellent analytical performance and holds promise as a novel tool for the early tracing and diagnosis of HTLV-1 DNA.
