Bacterial endotoxin(a type of lipopolysaccharide,LPS)that acts as the strongest immune stimulant exhibits high toxicity to human health.The golden standard detection methods rely heavily on the use of a large amount o...Bacterial endotoxin(a type of lipopolysaccharide,LPS)that acts as the strongest immune stimulant exhibits high toxicity to human health.The golden standard detection methods rely heavily on the use of a large amount of tachypleus amebocyte lysate(TAL)reagents,extracted from the unique blue blood of legally protected horseshoe crabs.Herein,a cost-effective distance-based lateral flow(D-LAF)sensor is demonstrated for the first time based on the coagulation cascade process of TAL induced by endotoxin,which causes the generation of gel-state TAL.The gelation process can increase the amount of trapped water molecules and shorten the lateral flow distance of the remaining free water on the pH paper.The water flow distance is directly correlated to the concentration of endotoxin.Noteworthy,the D-LAF sensor allows the detection of endotoxin with the reduced dosage of TAL reagents than the golden standard detection methods.The detection limit of endotoxin is calculated to be 0.0742 EU/mL.This method can be applied to the detection of endotoxin in real samples such as household water and clinical injection solution with excellent performance comparable to the commercial ELISA kit.展开更多
基金supported by the National Natural Science Foundation of China(No.T2250410382)Natural Science Foundation of Shandong Province(Nos.ZR2020QB153 and ZR2022YQ12)+3 种基金Taishan Scholars Program(Nos.tsqn201812088 and ts20190948)Shandong Scientific and Technical Small and Medium-sized Enterprises Innovation Capacity Improvement Project(No.2022TSGC2533)The Science,Education and Industry Integration of Basic Research Project of Qilu University of Technology(No.2023PY058)Qilu University of Technology Talent Research Project(No.2023RCKY087)。
文摘Bacterial endotoxin(a type of lipopolysaccharide,LPS)that acts as the strongest immune stimulant exhibits high toxicity to human health.The golden standard detection methods rely heavily on the use of a large amount of tachypleus amebocyte lysate(TAL)reagents,extracted from the unique blue blood of legally protected horseshoe crabs.Herein,a cost-effective distance-based lateral flow(D-LAF)sensor is demonstrated for the first time based on the coagulation cascade process of TAL induced by endotoxin,which causes the generation of gel-state TAL.The gelation process can increase the amount of trapped water molecules and shorten the lateral flow distance of the remaining free water on the pH paper.The water flow distance is directly correlated to the concentration of endotoxin.Noteworthy,the D-LAF sensor allows the detection of endotoxin with the reduced dosage of TAL reagents than the golden standard detection methods.The detection limit of endotoxin is calculated to be 0.0742 EU/mL.This method can be applied to the detection of endotoxin in real samples such as household water and clinical injection solution with excellent performance comparable to the commercial ELISA kit.
