探讨胆汁性肝硬化门脉高压症猪脾组织中内皮素受体 (ETR)及诱导型一氧化氮合酶 (i NOS) m RNA的表达及其对内脏血流动力学的影响。采用原位杂交方法检测实验组 (n=10 )和对照组 (n=5 )猪脾组织冰冻切片中 ETR(ETAR、 ETBR)及 i NOS m RN...探讨胆汁性肝硬化门脉高压症猪脾组织中内皮素受体 (ETR)及诱导型一氧化氮合酶 (i NOS) m RNA的表达及其对内脏血流动力学的影响。采用原位杂交方法检测实验组 (n=10 )和对照组 (n=5 )猪脾组织冰冻切片中 ETR(ETAR、 ETBR)及 i NOS m RNA的表达。同时监测猪的门脉血流量 (Qpv)、门静脉压 (Ppv)及平均动脉压 (MAP)。结果 ,实验组 ETAR m RNA表达水平与对照组无显著差异 (P>0 .0 5 ) ,而 ETBR及 i NOS m RNA表达水平显著高于对照组 (P<0 .0 1) ,其中 ETAR m RNA表达主要位于血管平滑肌细胞中 ;ETBR m RNA主要位于脾窦内皮细胞 ;i NOS m R-NA主要在血管平滑肌细胞、巨噬细胞和内皮细胞中。 ETAR m RNA的表达与 Qpv、 Ppv无明显相关 (P=0 .11或 P=0 .0 8) ;ETBR与 i NOS m RNA的表达水平分别与 Qpv、Ppv呈正相关 (P<0 .0 5或 P<0 .0 1) ;而分别与 MAP呈负相关 (P<0 .0 5或 P<0 .0 1)。研究表明 :ETBR及 i NOS m展开更多
We report the long-term follow-up of 3 cases of severe idiopathic pulmonary arterial hypertension,in whom tadalafil plus sitaxentan combination therapy improved the clinical condition and exercise performance without ...We report the long-term follow-up of 3 cases of severe idiopathic pulmonary arterial hypertension,in whom tadalafil plus sitaxentan combination therapy improved the clinical condition and exercise performance without any relevant adverse event.展开更多
The purpose of the present study was to assess the correlation that likely exists among increased portal pressure (P p), portal blood flow quantity (Q p) and ET A and ET B receptor mRNA expression in human cirrhos...The purpose of the present study was to assess the correlation that likely exists among increased portal pressure (P p), portal blood flow quantity (Q p) and ET A and ET B receptor mRNA expression in human cirrhosis. In situ hybridization and reverse transcription polymerase chain reactions (RT PCR) were performed to determine the expression of ET A and ET B receptor mRNA in liver tissues from traumatic subjects ( n =10) and cirrhotic patients ( n =15) in whom hepatic hemodynamic values were measured. The expression of the two transcripts was significantly higher in liver samples of cirrhotic patients than in those obtained from traumatic subjects. It has shown that ET A receptor mRNA predominantly located in hepatic stellate cells (HSCs) and vascular smooth muscle cells of intrahepatic arteries and portal veins, ET B receptor mRNA in HSCs, sinusoidal endothelial cells and Kuppfer cells. There was a highly significant direct relationship between ET A and ET B receptor mRNA and P p and Q p in cirrhotic patients. It suggests that liver paracrine endothelin system may be overactivated in human cirrhosis accompanied with increased expression of ET A and ET B receptor mRNA which may play an important role in the pathogenesis and maintenance of splanchnic hyperdynamics.展开更多
Objective To explore the role of endothelin (ET) in the pathogenesis of exercise induced asthma (EIA), we investigated the effects of ET B receptor antagonists, ET 1 11 21 fragment and N cis 2,6 dimethylp...Objective To explore the role of endothelin (ET) in the pathogenesis of exercise induced asthma (EIA), we investigated the effects of ET B receptor antagonists, ET 1 11 21 fragment and N cis 2,6 dimethylpi^peridinocardonyl L γ methylleucyl D 1 methoxycarbonyl tryptophanyl D norleucine (BQ788) on broncho^constriction elicited by isocapnic hyperpnea in guinea pigs Methods Eighteen pathogen free Hartley guinea pigs were randomly divided into three groups A: normal saline (NS) inhalation control group (n=6), B: BQ788 group (n=6), and C: ET 1 11 21 fragment group (n=6) Guinea pigs were anesthetized with pentobarbital sodium After measuring the basal value of lung resistance (R L) and dynamic compliance of the respiratory system (Cdyn), NS (0 96?ml), BQ788 (9?nmol) and ET 1 11 21 fragment (9?nmol) were inhaled A rodent respirator with a dry 5%CO 2-95%O 2 mixture at room temperature provided mechanical ventilation (V T 8?ml/animal, 100 breaths/min) for 5?min R L and Cdyn of the 3 groups were measured again after isocapnic hyperpnea challenge Results In the control group, isocapnic hyperpnea of dry gas elicited a marked increase in R L and decrease in Cdyn R L and Cdyn of the guinea pigs from BQ788 group and ET 1 11 21 fragment group did not change significantly Conclusion It was demonstrated that selective ET B receptor antagonists, ET 1 11 21 fragment and BQ788, inhibited the bronchoconstriction induced by isocapnic hyperpnea in guinea pigs The data showed that ETs are potent constrictors of guinea pig airway smooth muscle via a direct effect on ET receptors It was suggested that ET receptor antagonists, especially ET B receptor antagonist, might be beneficial in preventing EIA展开更多
INTRODUCTIONEndothelins(ETs) has a potent and sustainedvasoconstrictive effect on a variety of blood vessels.The vascular smooth muscle cell (VSMC)is thetarget for ETs.VSMC of the whole body containsendothelin recepto...INTRODUCTIONEndothelins(ETs) has a potent and sustainedvasoconstrictive effect on a variety of blood vessels.The vascular smooth muscle cell (VSMC)is thetarget for ETs.VSMC of the whole body containsendothelin receptor (ETR).A great number ofexperiments have shown that three distinctcomplementary DNAs of ETR have been identifiedi.e.,endothelin A receptor(ET_A receptor),endothelin B receptor(ET_B receptor)展开更多
文摘探讨胆汁性肝硬化门脉高压症猪脾组织中内皮素受体 (ETR)及诱导型一氧化氮合酶 (i NOS) m RNA的表达及其对内脏血流动力学的影响。采用原位杂交方法检测实验组 (n=10 )和对照组 (n=5 )猪脾组织冰冻切片中 ETR(ETAR、 ETBR)及 i NOS m RNA的表达。同时监测猪的门脉血流量 (Qpv)、门静脉压 (Ppv)及平均动脉压 (MAP)。结果 ,实验组 ETAR m RNA表达水平与对照组无显著差异 (P>0 .0 5 ) ,而 ETBR及 i NOS m RNA表达水平显著高于对照组 (P<0 .0 1) ,其中 ETAR m RNA表达主要位于血管平滑肌细胞中 ;ETBR m RNA主要位于脾窦内皮细胞 ;i NOS m R-NA主要在血管平滑肌细胞、巨噬细胞和内皮细胞中。 ETAR m RNA的表达与 Qpv、 Ppv无明显相关 (P=0 .11或 P=0 .0 8) ;ETBR与 i NOS m RNA的表达水平分别与 Qpv、Ppv呈正相关 (P<0 .0 5或 P<0 .0 1) ;而分别与 MAP呈负相关 (P<0 .0 5或 P<0 .0 1)。研究表明 :ETBR及 i NOS m
文摘We report the long-term follow-up of 3 cases of severe idiopathic pulmonary arterial hypertension,in whom tadalafil plus sitaxentan combination therapy improved the clinical condition and exercise performance without any relevant adverse event.
基金This project was supported by a grant from national Nat-ural Scienc Foundation of China (No. 39470 6 85 )
文摘The purpose of the present study was to assess the correlation that likely exists among increased portal pressure (P p), portal blood flow quantity (Q p) and ET A and ET B receptor mRNA expression in human cirrhosis. In situ hybridization and reverse transcription polymerase chain reactions (RT PCR) were performed to determine the expression of ET A and ET B receptor mRNA in liver tissues from traumatic subjects ( n =10) and cirrhotic patients ( n =15) in whom hepatic hemodynamic values were measured. The expression of the two transcripts was significantly higher in liver samples of cirrhotic patients than in those obtained from traumatic subjects. It has shown that ET A receptor mRNA predominantly located in hepatic stellate cells (HSCs) and vascular smooth muscle cells of intrahepatic arteries and portal veins, ET B receptor mRNA in HSCs, sinusoidal endothelial cells and Kuppfer cells. There was a highly significant direct relationship between ET A and ET B receptor mRNA and P p and Q p in cirrhotic patients. It suggests that liver paracrine endothelin system may be overactivated in human cirrhosis accompanied with increased expression of ET A and ET B receptor mRNA which may play an important role in the pathogenesis and maintenance of splanchnic hyperdynamics.
文摘Objective To explore the role of endothelin (ET) in the pathogenesis of exercise induced asthma (EIA), we investigated the effects of ET B receptor antagonists, ET 1 11 21 fragment and N cis 2,6 dimethylpi^peridinocardonyl L γ methylleucyl D 1 methoxycarbonyl tryptophanyl D norleucine (BQ788) on broncho^constriction elicited by isocapnic hyperpnea in guinea pigs Methods Eighteen pathogen free Hartley guinea pigs were randomly divided into three groups A: normal saline (NS) inhalation control group (n=6), B: BQ788 group (n=6), and C: ET 1 11 21 fragment group (n=6) Guinea pigs were anesthetized with pentobarbital sodium After measuring the basal value of lung resistance (R L) and dynamic compliance of the respiratory system (Cdyn), NS (0 96?ml), BQ788 (9?nmol) and ET 1 11 21 fragment (9?nmol) were inhaled A rodent respirator with a dry 5%CO 2-95%O 2 mixture at room temperature provided mechanical ventilation (V T 8?ml/animal, 100 breaths/min) for 5?min R L and Cdyn of the 3 groups were measured again after isocapnic hyperpnea challenge Results In the control group, isocapnic hyperpnea of dry gas elicited a marked increase in R L and decrease in Cdyn R L and Cdyn of the guinea pigs from BQ788 group and ET 1 11 21 fragment group did not change significantly Conclusion It was demonstrated that selective ET B receptor antagonists, ET 1 11 21 fragment and BQ788, inhibited the bronchoconstriction induced by isocapnic hyperpnea in guinea pigs The data showed that ETs are potent constrictors of guinea pig airway smooth muscle via a direct effect on ET receptors It was suggested that ET receptor antagonists, especially ET B receptor antagonist, might be beneficial in preventing EIA
文摘INTRODUCTIONEndothelins(ETs) has a potent and sustainedvasoconstrictive effect on a variety of blood vessels.The vascular smooth muscle cell (VSMC)is thetarget for ETs.VSMC of the whole body containsendothelin receptor (ETR).A great number ofexperiments have shown that three distinctcomplementary DNAs of ETR have been identifiedi.e.,endothelin A receptor(ET_A receptor),endothelin B receptor(ET_B receptor)
