用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,1...用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,10号白油为佐剂,制成ND-IB-EDS76-AE四联灭活疫苗。对四联苗各项技术指标进行了测定,证明本疫苗安全、无任何副作用;免疫10-14 d后产生免疫力;ND部分效检,攻毒100％保护,每羽份含ND效价达50-123 PD50;IB部分效检,免疫21-28 d后攻毒保护率达80％-100％,IB HI效价≥1:64;EDS76效检,免疫后21 d HI效价≥1:128;AE效检,保护率达80％-100％。展开更多
The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases.The gene was then directionally ...The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases.The gene was then directionally inserted into the PphI/KpnI sites of pQE32 and fused with the 6×His gene.This recombinant plasmid was transformed into E.coli M15 for expression and induced with IPTG.It was demonstrated by SDS-PAGE and Western blot that one expressed protein,110kD in size,could be specifically recognized by polyclonal anti-EDSV serum.The objective product was principally soluble and accounted for 21% of the total cellular proteins.The protein was used to detect the existence of antiEDSV IgG in 41 clinical chicken sera by agar diffusion test,and the result was in accordance with that when EDSV was used as antigen.These results showed that the expressed hexon recombinant protein can be used as diagnostic antigen of EDSV.展开更多
文摘用NDV La Sota株、IBV M41株、EDS76 V HSH23株、AEV Van Roekel株为种毒,分别接种鸡胚或鸭胚,制备各种病毒抗原液,经福尔马林灭活后,采用FILTRON盒式超滤系统对制苗病毒抗原液进行浓缩,然后按一定比例混合,以司本80及吐温80为乳化剂,10号白油为佐剂,制成ND-IB-EDS76-AE四联灭活疫苗。对四联苗各项技术指标进行了测定,证明本疫苗安全、无任何副作用;免疫10-14 d后产生免疫力;ND部分效检,攻毒100％保护,每羽份含ND效价达50-123 PD50;IB部分效检,免疫21-28 d后攻毒保护率达80％-100％,IB HI效价≥1:64;EDS76效检,免疫后21 d HI效价≥1:128;AE效检,保护率达80％-100％。
文摘The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases.The gene was then directionally inserted into the PphI/KpnI sites of pQE32 and fused with the 6×His gene.This recombinant plasmid was transformed into E.coli M15 for expression and induced with IPTG.It was demonstrated by SDS-PAGE and Western blot that one expressed protein,110kD in size,could be specifically recognized by polyclonal anti-EDSV serum.The objective product was principally soluble and accounted for 21% of the total cellular proteins.The protein was used to detect the existence of antiEDSV IgG in 41 clinical chicken sera by agar diffusion test,and the result was in accordance with that when EDSV was used as antigen.These results showed that the expressed hexon recombinant protein can be used as diagnostic antigen of EDSV.
