BACKGROUND The incidence of breast cancer in China is increasing while its mortality rate is decreasing.The annual breast cancer incidence in China is 39.2 million,accounting for two-thirds of the urban population.In ...BACKGROUND The incidence of breast cancer in China is increasing while its mortality rate is decreasing.The annual breast cancer incidence in China is 39.2 million,accounting for two-thirds of the urban population.In China,breast cancer is the fifth most common malignant tumor overall and the most common in women,accounting for 17%of female malignant tumors.AIM To investigate the accuracy of strain ultrasound elastography(SUE)on the evaluation of preoperative neoadjuvant chemotherapy(NAC)in breast cancer.METHODS Overall,90 patients with breast cancer treated at our hospital between January 2018 and February 2019 were selected for this study.The patients received six cycles of NAC with docetaxel,epirubicin,and cyclophosphamide.Surgical treatment was also performed,and pathological reactivity was assessed.The patients were evaluated using conventional ultrasonography and SUE before biopsy.The differences between groups were analyzed to calculate the mean and standard deviation with significance measured using a t-test,while multivariate analysis was performed using logistic regression analysis.RESULTS Of the patients analyzed,20 had a pathological complete remission(pCR)while 70 did not achieve pCR after NAC.The ratio of the elastic strain ratio(SR)and elastic score of 4–5 in patients with pCR were 5.5±1.16 and 15.00%,respectively;these were significantly lower than those in patients without pCR(85%)and significantly higher than in patients without pCR(14%).SR and elastic score 4–5 were independent factors influencing NAC efficacy(OR=0.644,1.426 and 1.366,respectively,P<0.05).SR was positively correlated with elasticity score(rs=0.411,P<0.05).The area under the receiver operator characteristic curve of SR and SR combined with elastic score in predicting patients without pCR was 0.822 and 0.891,respectively(P<0.05).CONCLUSION Strain ultrasonic elastography may be used to evaluate the effects of preoperative NAC in patients with breast cancer.展开更多
Objective： To observe the effect of the combination of Wenxia Changfu Formula （温下肠腑方, WCF） with cisplatin （CDDP） on inhibiting non-small cell lung cancer （NSCLC） in vitro and in vivo and explore its mechan...Objective： To observe the effect of the combination of Wenxia Changfu Formula （温下肠腑方, WCF） with cisplatin （CDDP） on inhibiting non-small cell lung cancer （NSCLC） in vitro and in vivo and explore its mechanism from its effect on cell cycle. Methods： In vitro, WCF-containing serum was prepared and the rhubarb bl, emodin, and aconitine were detected qualitatively by high-performance liquid chromatogram （HPLC）. A549 cell lines were treated with blank control （dimethyl sulfoxide）, normal serum, normal serum with CDDP （1.25, 2.5, and 5.0 μg/mL, respectively）, WCF-containing serum plus different doses of CDDP （1.25, 2.5, and 5.0 μ g/mL, respectively）. The inhibitory effect was detected by 3-（4,5）-dimethylthiazo（-z- yl）-3,5-diphenylterazolium bromide （MI-I）. The cell cycle was detected by flow cytometry. The protein and mRNA expressions of cyciin D1, proliferating cell nuclear antigen （PCNA）, retinoblastoma （Rb）, and p16 were observed with immunofluorescence and RT-PCR, respectively. In vivo, nude mice xenograft model was established and grouped into the control, CDDP, WCF, and combination groups. The combination＇s inhibition of tumor growth and influence on the weight, spleen, and thymus gland were observed. Results： The inhibitory rate of the combination against A549 cell lines excelled the CDDP alone significantly （P〈0.05）; the combination showed a synergism inhibitory effect （Q=1.19）. Compared with the monotherapy, the combination increased the cell percentage in G0/G1 phase and decreased the cell percentage in S phase significantly （P〈0.05）; the protein and mRNA expressions of cyclin D1, PCNA, and Rb were significantly reduced; the protein and mRNA expressions of p16 were significantly enhanced. Compared with the monotherapy, the combination inhibited the tumor growth significantly in vivo and reduced the weight of tumor （P〈0.05）; compared with the CDDP group, the spleen and thymus gland index of the combination group were enha展开更多
文摘BACKGROUND The incidence of breast cancer in China is increasing while its mortality rate is decreasing.The annual breast cancer incidence in China is 39.2 million,accounting for two-thirds of the urban population.In China,breast cancer is the fifth most common malignant tumor overall and the most common in women,accounting for 17%of female malignant tumors.AIM To investigate the accuracy of strain ultrasound elastography(SUE)on the evaluation of preoperative neoadjuvant chemotherapy(NAC)in breast cancer.METHODS Overall,90 patients with breast cancer treated at our hospital between January 2018 and February 2019 were selected for this study.The patients received six cycles of NAC with docetaxel,epirubicin,and cyclophosphamide.Surgical treatment was also performed,and pathological reactivity was assessed.The patients were evaluated using conventional ultrasonography and SUE before biopsy.The differences between groups were analyzed to calculate the mean and standard deviation with significance measured using a t-test,while multivariate analysis was performed using logistic regression analysis.RESULTS Of the patients analyzed,20 had a pathological complete remission(pCR)while 70 did not achieve pCR after NAC.The ratio of the elastic strain ratio(SR)and elastic score of 4–5 in patients with pCR were 5.5±1.16 and 15.00%,respectively;these were significantly lower than those in patients without pCR(85%)and significantly higher than in patients without pCR(14%).SR and elastic score 4–5 were independent factors influencing NAC efficacy(OR=0.644,1.426 and 1.366,respectively,P<0.05).SR was positively correlated with elasticity score(rs=0.411,P<0.05).The area under the receiver operator characteristic curve of SR and SR combined with elastic score in predicting patients without pCR was 0.822 and 0.891,respectively(P<0.05).CONCLUSION Strain ultrasonic elastography may be used to evaluate the effects of preoperative NAC in patients with breast cancer.
基金Supported by the National Natural Science Foundation of China (No.81072832N0.30873222)Shandong Provincial Young and Middle-aged Scientists(No.2008BS03010)
文摘Objective： To observe the effect of the combination of Wenxia Changfu Formula （温下肠腑方, WCF） with cisplatin （CDDP） on inhibiting non-small cell lung cancer （NSCLC） in vitro and in vivo and explore its mechanism from its effect on cell cycle. Methods： In vitro, WCF-containing serum was prepared and the rhubarb bl, emodin, and aconitine were detected qualitatively by high-performance liquid chromatogram （HPLC）. A549 cell lines were treated with blank control （dimethyl sulfoxide）, normal serum, normal serum with CDDP （1.25, 2.5, and 5.0 μg/mL, respectively）, WCF-containing serum plus different doses of CDDP （1.25, 2.5, and 5.0 μ g/mL, respectively）. The inhibitory effect was detected by 3-（4,5）-dimethylthiazo（-z- yl）-3,5-diphenylterazolium bromide （MI-I）. The cell cycle was detected by flow cytometry. The protein and mRNA expressions of cyciin D1, proliferating cell nuclear antigen （PCNA）, retinoblastoma （Rb）, and p16 were observed with immunofluorescence and RT-PCR, respectively. In vivo, nude mice xenograft model was established and grouped into the control, CDDP, WCF, and combination groups. The combination＇s inhibition of tumor growth and influence on the weight, spleen, and thymus gland were observed. Results： The inhibitory rate of the combination against A549 cell lines excelled the CDDP alone significantly （P〈0.05）; the combination showed a synergism inhibitory effect （Q=1.19）. Compared with the monotherapy, the combination increased the cell percentage in G0/G1 phase and decreased the cell percentage in S phase significantly （P〈0.05）; the protein and mRNA expressions of cyclin D1, PCNA, and Rb were significantly reduced; the protein and mRNA expressions of p16 were significantly enhanced. Compared with the monotherapy, the combination inhibited the tumor growth significantly in vivo and reduced the weight of tumor （P〈0.05）; compared with the CDDP group, the spleen and thymus gland index of the combination group were enha
