The genome editing tool,clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9 system,has achieved successful therapeutic efficacy via precise modification of the genome and exceeded previous genome en...The genome editing tool,clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9 system,has achieved successful therapeutic efficacy via precise modification of the genome and exceeded previous genome engineering methods owing to its versatility and simplicity.Rapid expansion in biomedical research has benefited from this newly emerged technique,such as genetic diseases treatment,cancer characterization,and plant improvement.However,the key challenge is efficient delivery of CRISPR components in vivo and nanotechnology plays an in dispensable role in non viral gene delivery.In this review,we will first briefly describe the mechanism and delivery strategies of CRISPR/Cas9 system.Furthermore,the past and current researches of nan oparticles based CRISPR/Cas9 system delivery for genome editi ng will be highlighted.Fin ally,we will discuss the challe nges and prospects of CRISPR/Cas9 system combi ned with nano tech no logy for clinical translation in the future.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.81673374 and 81872810)Wuhan Science and Technology Plan for Applied Fundamental Research(No.2017060201010146)Fundamental Research Funds for the Central Universities(No.2018KFYYXJJ019).
文摘The genome editing tool,clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9 system,has achieved successful therapeutic efficacy via precise modification of the genome and exceeded previous genome engineering methods owing to its versatility and simplicity.Rapid expansion in biomedical research has benefited from this newly emerged technique,such as genetic diseases treatment,cancer characterization,and plant improvement.However,the key challenge is efficient delivery of CRISPR components in vivo and nanotechnology plays an in dispensable role in non viral gene delivery.In this review,we will first briefly describe the mechanism and delivery strategies of CRISPR/Cas9 system.Furthermore,the past and current researches of nan oparticles based CRISPR/Cas9 system delivery for genome editi ng will be highlighted.Fin ally,we will discuss the challe nges and prospects of CRISPR/Cas9 system combi ned with nano tech no logy for clinical translation in the future.
