t Successful pregnancy in placental mammals substantially depends on the establishment of maternal immune tolerance to the semi-allogenic fetus.Disorders in this process are tightly associated with adverse pregnancy o...t Successful pregnancy in placental mammals substantially depends on the establishment of maternal immune tolerance to the semi-allogenic fetus.Disorders in this process are tightly associated with adverse pregnancy outcomes including recurrent miscarriage(RM).However,an indepth understanding of the systematic and decidual immune environment in RM remains largely lacking.In this study,we utilized single-cell RNA-sequencing(scRNA-seq)to comparably analyze the cellular and molecular signatures of decidual and peripheral leukocytes in normal and unexplained RM pregnancies at the early stage of gestation.Integrative analysis identifies 22 distinct cell clusters in total,and a dramatic difference in leukocyte subsets and molecular properties in RM cases is revealed.Specifically,the cytotoxic properties of CD8^(+)effector T cells,nature killer(NK),and mucosal-associated invariant T(MAIT)cells in peripheral blood indicates apparently enhanced pro-inflammatory status,and the population proportions and ligand–receptor interactions of the decidual leukocyte subsets demonstrate preferential immune activation in RM patients.The molecular features,spatial distribution,and the developmental trajectories of five decidual NK(dNK)subsets have been elaborately illustrated.In RM patients,a dNK subset that supports embryonic growth is diminished in proportion,while the ratio of another dNK subset with cyto toxic and immune-active signature is significantly increased.Notably,a unique pro-inflammatory CD56^(+)CD16^(+)dNK subset substantially accumulates in RM decidua.These findings reveal a comprehensive cellular and molecular atlas of decidual and peripheral leukocytes in human early pregnancy and provide an in-depth insight into the immune pathogenesis for early pregnancy loss.展开更多
目的:基于前瞻性队列比较不同方法确定的儿童早期体重指数(body mass index,BMI)轨迹与超重风险的关联,识别在儿童早期生长发育重要窗口期具有较高肥胖风险的人群。方法:共纳入北大通州出生队列的1330名儿童,分别在儿童刚出生,1、3、6、...目的:基于前瞻性队列比较不同方法确定的儿童早期体重指数(body mass index,BMI)轨迹与超重风险的关联,识别在儿童早期生长发育重要窗口期具有较高肥胖风险的人群。方法:共纳入北大通州出生队列的1330名儿童,分别在儿童刚出生,1、3、6、9、12、18、24月龄和3岁进行随访,根据其身长/身高和体质量计算BMI Z评分。应用潜类别增长混合模型(growth mixture modelling,GMM)和基于纵向数据的k-means聚类方法(k-means for longitudinal data,KML)分析儿童早期(从出生至24月龄)BMI轨迹分组,采用线性回归比较不同方法确定的儿童早期BMI轨迹和儿童3岁时BMI Z评分的关联,通过五折交叉验证的平均受试者工作特征曲线下面积,比较Logistic回归模型中不同方法确定的儿童早期BMI轨迹分组对儿童3岁超重风险(BMI Z评分>1)的预测性能。结果:在纳入的研究对象中,用GMM确定的三分类轨迹分为低、中、高轨迹,分别占39.7%、54.1%、6.2%;用KML方法确定的二分类轨迹分为低轨迹和高轨迹,分别占50.3%和49.7%;用KML方法确定的三分类轨迹分为低、中、高轨迹,分别占31.1%、47.4%、21.5%。用不同方法确定的儿童早期BMI轨迹反映的生长模式存在一定差异。线性回归分析发现,在调整母亲民族、受教育水平、分娩方式、产次、分娩时年龄、分娩孕周、胎儿性别和1月龄母乳喂养等协变量后,用KML方法确定的三分类轨迹中的高轨迹组(表现为出生时BMI Z评分略高、婴儿期快速生长后持续稳定在高水平的生长模式)和儿童3岁BMI Z评分的关联最强。Logistic回归分析发现,KML三分类轨迹分组对儿童3岁超重风险具有最佳的预测效果。在额外调整儿童平衡膳食指数正端分、平均每天身体活动时间和视屏时间后,结果基本一致。结论:采用不同方法识别具有不同变化特征的儿童早期BMI轨迹,发现KML方法确定的高轨迹组能更好地发现儿童早�展开更多
BACKGROUND Cerebral infarction is a local or extensive necrosis of brain tissue.Subsequently,the corresponding neurological deficits appear.The incidence of cerebrovascular diseases in China is increasing gradually.Af...BACKGROUND Cerebral infarction is a local or extensive necrosis of brain tissue.Subsequently,the corresponding neurological deficits appear.The incidence of cerebrovascular diseases in China is increasing gradually.After the onset of cerebrovascular disease,the most common sequelae include movement disorders,language disorders,and cognitive dysfunction.AIM To investigate the effect of early refined nursing program on the prognosis of middle-aged and elderly patients with cerebral infarction combined with cognitive dysfunction.METHODS A retrospective study was conducted to divide 60 patients with cerebral infarction and cognitive impairment into an experimental group(n=32)and a control group(n=28).The experimental group received early intensive care every day,and the control group received daily routine care.The scores of the Mini-Mental State Examination(MMSE)and the Trail Making Test(TMT),as well as the latency and amplitude of the event-related potential P300,were used as main indicators to evaluate changes in cognitive function,and changes in BDNF,TGF-β,and GDNF expression were used as secondary indicators.RESULTS Both groups experienced notable enhancements in MMSE scores,with the experi-mental group demonstrating higher scores than the control group(experimental:28.75±2.31;control:25.84±2.87).Moreover,reductions in TMT-A and TMT-B scores were observed in both groups(experimental:TMT-A 52.36±6.18,TMT-B 98.47±10.23;control:TMT-A 61.48±7.92,TMT-B 112.63±12.55),with the experimental group displaying lower scores.P300 Latency decreased(experimental:270.63 ms±14.28 ms;control:285.72 ms±16.45 ms),while amplitude increased(experimental:7.82μV±1.05μV;control:6.35μV±0.98μV)significantly in both groups,with superior outcomes in the experimental cohort.Additionally,the levels of the growth factors BDNF,TGF-β1,and GDNF surged(experimental:BDNF 48.37 ng/mL±5.62 ng/mL,TGF-β152.14 pg/mL±4.28 pg/mL,GDNF 34.76 ng/mL±3.89 ng/mL;control:BDNF 42.58 ng/mL±4.73 ng/mL,TGF-β146.23 pg/mL±3.94 pg/mL,GDNF 30.25 n展开更多
Under single-satellite observation,the parameter estimation of the boost phase of high-precision space noncooperative targets requires prior information.To improve the accuracy without prior information,we propose a p...Under single-satellite observation,the parameter estimation of the boost phase of high-precision space noncooperative targets requires prior information.To improve the accuracy without prior information,we propose a parameter estimation model of the boost phase based on trajectory plane parametric cutting.The use of the plane passing through the geo-center and the cutting sequence line of sight(LOS)generates the trajectory-cutting plane.With the coefficient of the trajectory cutting plane directly used as the parameter to be estimated,a motion parameter estimation model in space non-cooperative targets is established,and the Gauss-Newton iteration method is used to solve the flight parameters.The experimental results show that the estimation algorithm proposed in this paper weakly relies on prior information and has higher estimation accuracy,providing a practical new idea and method for the parameter estimation of space non-cooperative targets under single-satellite warning.展开更多
基金the National Key R&D Program of China(Grant Nos.2018YFC1004100,2017YFC1001404,2016YFC1000401,and 2016YFC1000200)the National Natural Science Foundation of China(Grant Nos.81730040and 81490740)。
文摘t Successful pregnancy in placental mammals substantially depends on the establishment of maternal immune tolerance to the semi-allogenic fetus.Disorders in this process are tightly associated with adverse pregnancy outcomes including recurrent miscarriage(RM).However,an indepth understanding of the systematic and decidual immune environment in RM remains largely lacking.In this study,we utilized single-cell RNA-sequencing(scRNA-seq)to comparably analyze the cellular and molecular signatures of decidual and peripheral leukocytes in normal and unexplained RM pregnancies at the early stage of gestation.Integrative analysis identifies 22 distinct cell clusters in total,and a dramatic difference in leukocyte subsets and molecular properties in RM cases is revealed.Specifically,the cytotoxic properties of CD8^(+)effector T cells,nature killer(NK),and mucosal-associated invariant T(MAIT)cells in peripheral blood indicates apparently enhanced pro-inflammatory status,and the population proportions and ligand–receptor interactions of the decidual leukocyte subsets demonstrate preferential immune activation in RM patients.The molecular features,spatial distribution,and the developmental trajectories of five decidual NK(dNK)subsets have been elaborately illustrated.In RM patients,a dNK subset that supports embryonic growth is diminished in proportion,while the ratio of another dNK subset with cyto toxic and immune-active signature is significantly increased.Notably,a unique pro-inflammatory CD56^(+)CD16^(+)dNK subset substantially accumulates in RM decidua.These findings reveal a comprehensive cellular and molecular atlas of decidual and peripheral leukocytes in human early pregnancy and provide an in-depth insight into the immune pathogenesis for early pregnancy loss.
文摘BACKGROUND Cerebral infarction is a local or extensive necrosis of brain tissue.Subsequently,the corresponding neurological deficits appear.The incidence of cerebrovascular diseases in China is increasing gradually.After the onset of cerebrovascular disease,the most common sequelae include movement disorders,language disorders,and cognitive dysfunction.AIM To investigate the effect of early refined nursing program on the prognosis of middle-aged and elderly patients with cerebral infarction combined with cognitive dysfunction.METHODS A retrospective study was conducted to divide 60 patients with cerebral infarction and cognitive impairment into an experimental group(n=32)and a control group(n=28).The experimental group received early intensive care every day,and the control group received daily routine care.The scores of the Mini-Mental State Examination(MMSE)and the Trail Making Test(TMT),as well as the latency and amplitude of the event-related potential P300,were used as main indicators to evaluate changes in cognitive function,and changes in BDNF,TGF-β,and GDNF expression were used as secondary indicators.RESULTS Both groups experienced notable enhancements in MMSE scores,with the experi-mental group demonstrating higher scores than the control group(experimental:28.75±2.31;control:25.84±2.87).Moreover,reductions in TMT-A and TMT-B scores were observed in both groups(experimental:TMT-A 52.36±6.18,TMT-B 98.47±10.23;control:TMT-A 61.48±7.92,TMT-B 112.63±12.55),with the experimental group displaying lower scores.P300 Latency decreased(experimental:270.63 ms±14.28 ms;control:285.72 ms±16.45 ms),while amplitude increased(experimental:7.82μV±1.05μV;control:6.35μV±0.98μV)significantly in both groups,with superior outcomes in the experimental cohort.Additionally,the levels of the growth factors BDNF,TGF-β1,and GDNF surged(experimental:BDNF 48.37 ng/mL±5.62 ng/mL,TGF-β152.14 pg/mL±4.28 pg/mL,GDNF 34.76 ng/mL±3.89 ng/mL;control:BDNF 42.58 ng/mL±4.73 ng/mL,TGF-β146.23 pg/mL±3.94 pg/mL,GDNF 30.25 n
基金supported in part by the National Natural Science Foundation of China(Nos.42271448,41701531)the Key Laboratory of Land Satellite Remote Sensing Application,Ministry of Natural Resources of the People’s Republic of China(No.KLSMNRG202317)。
文摘Under single-satellite observation,the parameter estimation of the boost phase of high-precision space noncooperative targets requires prior information.To improve the accuracy without prior information,we propose a parameter estimation model of the boost phase based on trajectory plane parametric cutting.The use of the plane passing through the geo-center and the cutting sequence line of sight(LOS)generates the trajectory-cutting plane.With the coefficient of the trajectory cutting plane directly used as the parameter to be estimated,a motion parameter estimation model in space non-cooperative targets is established,and the Gauss-Newton iteration method is used to solve the flight parameters.The experimental results show that the estimation algorithm proposed in this paper weakly relies on prior information and has higher estimation accuracy,providing a practical new idea and method for the parameter estimation of space non-cooperative targets under single-satellite warning.
