Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening...Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation(BiFC)platform for protein-protein interaction screens and epiblast-like cell(EpiLC)-induction assays using reporter mouse embryonic stem cells(mESCs).Investigation of candidate interaction partners of core human pluripotent factors OCT4,NANOG,KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell(PGC)-inducing factors including BEN-domain(BEND/Bend)family members.Through RNA-seq,ChIP-seq,and ATAC-seq analyses,we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro.Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development.展开更多
In Taiwan,the low birth rate has become one of the most critical problems faced by the government and educational institutions at all levels.The enrolling student number of kindergartens perhaps is the most directly a...In Taiwan,the low birth rate has become one of the most critical problems faced by the government and educational institutions at all levels.The enrolling student number of kindergartens perhaps is the most directly affected by such trend.The purpose of this study aims at constructing a system dynamics model to depict the relationships between the preschool children and their stakeholders for deducing the evolutionary trends of,and the interactions of,governmental policies and the operations of the early childhood education institutions.Through the interpretation of policies and simulation analysis of the twenty-year growth trend related to the child population in Taiwan,this study found that governmental policies and the promotion of education and care services have a positive effect on the growth of all levels of kindergarten classes.Besides,the relationship between the education and care service staff and the kindergarten play a causal balancing role in our proposed model.We suggest that the system dynamics model proposed by this study can help to observe the dynamic relationships formed by the stakeholders in preschool education and care system based on the“joint responsibility”of Taiwan’s early childhood education.展开更多
基金the National Key R&D Program of China(2017YFA0102801)The National Natural Science Foundation of China(Grant Nos.31930058,31671540,32170802,and 31301082)+1 种基金Natural Science Foundation of Guangdong Province(2015B020228002,2017A030313093)Guangdong Basic and Applied Basic Research Foundation(2019A1515011422,2021A1515010759).
文摘Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation(BiFC)platform for protein-protein interaction screens and epiblast-like cell(EpiLC)-induction assays using reporter mouse embryonic stem cells(mESCs).Investigation of candidate interaction partners of core human pluripotent factors OCT4,NANOG,KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell(PGC)-inducing factors including BEN-domain(BEND/Bend)family members.Through RNA-seq,ChIP-seq,and ATAC-seq analyses,we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro.Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development.
文摘In Taiwan,the low birth rate has become one of the most critical problems faced by the government and educational institutions at all levels.The enrolling student number of kindergartens perhaps is the most directly affected by such trend.The purpose of this study aims at constructing a system dynamics model to depict the relationships between the preschool children and their stakeholders for deducing the evolutionary trends of,and the interactions of,governmental policies and the operations of the early childhood education institutions.Through the interpretation of policies and simulation analysis of the twenty-year growth trend related to the child population in Taiwan,this study found that governmental policies and the promotion of education and care services have a positive effect on the growth of all levels of kindergarten classes.Besides,the relationship between the education and care service staff and the kindergarten play a causal balancing role in our proposed model.We suggest that the system dynamics model proposed by this study can help to observe the dynamic relationships formed by the stakeholders in preschool education and care system based on the“joint responsibility”of Taiwan’s early childhood education.
