DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(T...DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(TRSV) and Cucumber mosaic virus(CMV) were designed and applied to the detection of the main potato viruses with multiplex reverse transcription polymerase chain reaction(m-RT-PCR),including two quarantine viruses in China(PVV and TRSV).The results showed that DPO-m-RT-PCR exhi-bited high PCR specificity and sensitivity even under less than optimal PCR condition compared with conventional m-RT-PCR.DPO-m-RT-PCR could be applied in the healthy evaluation of seed potato and its exit-entry quarantine.展开更多
文摘DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(TRSV) and Cucumber mosaic virus(CMV) were designed and applied to the detection of the main potato viruses with multiplex reverse transcription polymerase chain reaction(m-RT-PCR),including two quarantine viruses in China(PVV and TRSV).The results showed that DPO-m-RT-PCR exhi-bited high PCR specificity and sensitivity even under less than optimal PCR condition compared with conventional m-RT-PCR.DPO-m-RT-PCR could be applied in the healthy evaluation of seed potato and its exit-entry quarantine.
