Purpose： We present a systematic review of the literature concerning major aspects of science mapping to serve two primary purposes： First, to demonstrate the use of a science mapping approach to perform the review ...Purpose： We present a systematic review of the literature concerning major aspects of science mapping to serve two primary purposes： First, to demonstrate the use of a science mapping approach to perform the review so that researchers may apply the procedure to the review of a scientific domain of their own interest, and second, to identify major areas of research activities concerning science mapping, intellectual milestones in the development of key specialties, evolutionary stages of major specialties involved, and the dynamics of transitions from one specialty to another.Design/methodology/approach： We first introduce a theoretical framework of the evolution of a scientific specialty. Then we demonstrate a generic search strategy that can be used to construct a representative dataset of bibliographic records of a domain of research. Next, progressively synthesized co-citation networks are constructed and visualized to aid visual analytic studies of the domain＇s structural and dynamic patterns and trends. Finally, trajectories of citations made by particular types of authors and articles are presented to illustrate the predictive potential of the analytic approach.Findings： The evolution of the science mapping research involves the development of a number of interrelated specialties. Four major specialties are discussed in detail in terms of four evolutionary stages： conceptualization, tool construction, application, and codification. Underlying connections between major specialties are also explored. The predictive analysis demonstrates citations trajectories of potentially transformative contributions.Research limitations： The systematic review is primarily guided by citation patterns in the dataset retrieved from the literature. The scope of the data is limited by the source of the retrieval, i.e. the Web of Science, and the composite query used. An iterative query refinement is possible if one would like to improve the data quality, although the current approach serves our purpose adequately.展开更多
An intelligent manufacturing system is a composite intelligent system comprising humans,cyber systems,and physical systems with the aim of achieving specific manufacturing goals at an optimized level.This kind of inte...An intelligent manufacturing system is a composite intelligent system comprising humans,cyber systems,and physical systems with the aim of achieving specific manufacturing goals at an optimized level.This kind of intelligent system is called a human-cyber-physical system(HCPS).In terms of technology,HCPSs can both reveal technological principles and form the technological architecture for intelligent manufacturing.It can be concluded that the essence of intelligent manufacturing is to design,construct,and apply HCPSs in various cases and at different levels.With advances in information technology,intelligent manufacturing has passed through the stages of digital manufacturing and digital-networked manufacturing,and is evolving toward new-generation intelligent manufacturing(NGIM).NGIM is characterized by the in-depth integration of new-generation artificial intelligence(AI)technology(i.e.,enabling technology)with advanced manufacturing technology(i.e.,root technology);it is the core driving force of the new industrial revolution.In this study,the evolutionary footprint of intelligent manufacturing is reviewed from the perspective of HCPSs,and the implications,characteristics,technical frame,and key technologies of HCPSs for NGIM are then discussed in depth.Finally,an outlook of the major challenges of HCPSs for NGIM is proposed.展开更多
Alzheimer’s disease is the most common cause of dementia.It is an increasingly serious global health problem and has a significant impact on individuals and society.However,the precise cause of Alzheimer’s disease i...Alzheimer’s disease is the most common cause of dementia.It is an increasingly serious global health problem and has a significant impact on individuals and society.However,the precise cause of Alzheimer’s disease is still unknown.In this study,11,748 Web-of-Science-indexed manuscripts regarding Alzheimer’s disease,all published from 2015 to 2019,and their 693,938 references were analyzed.A document co-citation network map was drawn using CiteSpace software.Research frontiers and development trends were determined by retrieving subject headings with apparent changing word frequency trends,which can be used to forecast future research developments in Alzheimer’s disease.展开更多
After a brief review of studies on artificial boundaries in dynamic soil-structure interaction, a three-dimensional viscous-spring artificial boundary （VSAB） in the time domain is developed in this paper. First, the...After a brief review of studies on artificial boundaries in dynamic soil-structure interaction, a three-dimensional viscous-spring artificial boundary （VSAB） in the time domain is developed in this paper. First, the 3D VSAB equations in the normal and tangential directions are derived based on the elastic wave motion theory. Secondly, a numerical simulation technique of wave motion equations along with the VSAB condition in the time domain is studied. Finally, numerical examples of some classical elastic wave motion problems are presented and the results are compared with the associated theoretical solutions, demonstrating that high precision and adequate stability can be achieved by using the proposed 3D VSAB. The proposed 3D VSAB can be conveniently incorporated in the general finite element program, which is commonly used to study dynamic soil-structure interaction problems.展开更多
Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of ...Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of proteins in various organisms. It contains six predictors: Euk-mPLoc 2.0, Hum-mPLoc 2.0, Plant-mPLoc, Gpos-mPLoc, Gneg-mPLoc, and Virus-mPLoc, specialized for eukaryotic, human, plant, Gram- positive bacterial, Gram-negative bacterial, and virus proteins, respectively. Compared with Cell-PLoc, the predictors in the Cell-PLoc 2.0 have the following advantageous features: (1) they all have the capacity to deal with the multiplex proteins that can simultaneiously exist, or move between, two or more subcellular location sites;(2) no accession number is needed for the input of a query protein even if using the “high- level” GO (gene ontology) prediction engine;(3) the functional domain information and sequential evolution information are fused into the “ab initio” sequence-based prediction engine to enhance its accuracy. In this protocol, a step- to-step guide is provided for how to use the web server predictors in the Cell-PLoc 2.0 package, which is freely accessible to the public at http://www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc-2/.展开更多
Sex determining gene Mab-3 of C. elegans and doublesex of Drosophila contain a common DNA binding motif called a DM domain, both of which regulate similar aspects of sexual development. Human doublesex-related gene DM...Sex determining gene Mab-3 of C. elegans and doublesex of Drosophila contain a common DNA binding motif called a DM domain, both of which regulate similar aspects of sexual development. Human doublesex-related gene DMRT1 has been identified, which also contains the conserved DM-related DNA-binding domain and plays an essential role in gonadal differentiation. We present the amplification of a broad spectrum of DM domain sequences from phylogenetic diverse vertebrates (Cynops orientalis, Chrysemys scripta elegans and Coturnix coturnix) using degenerate PCR. Our results further reveal the unexpected complexity and the evolutionary conservation of the DM domain gene family.展开更多
The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulatio...The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.展开更多
The outbreak of Coronavirus Disease 2019(COVID-19)has posed a serious threat to global public health,calling for the development of safe and effective prophylactics and therapeutics against infection of its causative ...The outbreak of Coronavirus Disease 2019(COVID-19)has posed a serious threat to global public health,calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),also known as 2019 novel coronavirus(2019-nCoV).The CoV spike(S)protein plays the most important roles in viral attachment,fusion and entry,and serves as a target for development of antibodies,entry inhibitors and vaccines.Here,we identified the receptor-binding domain(RBD)in SARS-CoV-2 S protein and found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2(ACE2)receptors.SARS-CoV-2 RBD exhibited significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD and could block the binding and,hence,attachment of SARS-CoV-2 RBD and SARS-CoV RBD to ACE2-expressing cells,thus inhibiting their infection to host cells.SARS-CoV RBD-specific antibodies could crossreact with SARS-CoV-2 RBD protein,and SARS-CoV RBD-induced antisera could cross-neutralize SARS-CoV-2,suggesting the potential to develop SARS-CoV RBD-based vaccines for prevention of SARS-CoV-2 and SARS-CoV infection.展开更多
文摘Purpose： We present a systematic review of the literature concerning major aspects of science mapping to serve two primary purposes： First, to demonstrate the use of a science mapping approach to perform the review so that researchers may apply the procedure to the review of a scientific domain of their own interest, and second, to identify major areas of research activities concerning science mapping, intellectual milestones in the development of key specialties, evolutionary stages of major specialties involved, and the dynamics of transitions from one specialty to another.Design/methodology/approach： We first introduce a theoretical framework of the evolution of a scientific specialty. Then we demonstrate a generic search strategy that can be used to construct a representative dataset of bibliographic records of a domain of research. Next, progressively synthesized co-citation networks are constructed and visualized to aid visual analytic studies of the domain＇s structural and dynamic patterns and trends. Finally, trajectories of citations made by particular types of authors and articles are presented to illustrate the predictive potential of the analytic approach.Findings： The evolution of the science mapping research involves the development of a number of interrelated specialties. Four major specialties are discussed in detail in terms of four evolutionary stages： conceptualization, tool construction, application, and codification. Underlying connections between major specialties are also explored. The predictive analysis demonstrates citations trajectories of potentially transformative contributions.Research limitations： The systematic review is primarily guided by citation patterns in the dataset retrieved from the literature. The scope of the data is limited by the source of the retrieval, i.e. the Web of Science, and the composite query used. An iterative query refinement is possible if one would like to improve the data quality, although the current approach serves our purpose adequately.
文摘An intelligent manufacturing system is a composite intelligent system comprising humans,cyber systems,and physical systems with the aim of achieving specific manufacturing goals at an optimized level.This kind of intelligent system is called a human-cyber-physical system(HCPS).In terms of technology,HCPSs can both reveal technological principles and form the technological architecture for intelligent manufacturing.It can be concluded that the essence of intelligent manufacturing is to design,construct,and apply HCPSs in various cases and at different levels.With advances in information technology,intelligent manufacturing has passed through the stages of digital manufacturing and digital-networked manufacturing,and is evolving toward new-generation intelligent manufacturing(NGIM).NGIM is characterized by the in-depth integration of new-generation artificial intelligence(AI)technology(i.e.,enabling technology)with advanced manufacturing technology(i.e.,root technology);it is the core driving force of the new industrial revolution.In this study,the evolutionary footprint of intelligent manufacturing is reviewed from the perspective of HCPSs,and the implications,characteristics,technical frame,and key technologies of HCPSs for NGIM are then discussed in depth.Finally,an outlook of the major challenges of HCPSs for NGIM is proposed.
文摘Alzheimer’s disease is the most common cause of dementia.It is an increasingly serious global health problem and has a significant impact on individuals and society.However,the precise cause of Alzheimer’s disease is still unknown.In this study,11,748 Web-of-Science-indexed manuscripts regarding Alzheimer’s disease,all published from 2015 to 2019,and their 693,938 references were analyzed.A document co-citation network map was drawn using CiteSpace software.Research frontiers and development trends were determined by retrieving subject headings with apparent changing word frequency trends,which can be used to forecast future research developments in Alzheimer’s disease.
基金National Natural Science Foundation of ChinaUnder Grant No.50478014Special Funds for Major State Basic Research Project Under Grant No.2002CB412706Research Funds from National Civil Defense Oficce of Chinafor the Tenth Five-year Plan。
文摘After a brief review of studies on artificial boundaries in dynamic soil-structure interaction, a three-dimensional viscous-spring artificial boundary （VSAB） in the time domain is developed in this paper. First, the 3D VSAB equations in the normal and tangential directions are derived based on the elastic wave motion theory. Secondly, a numerical simulation technique of wave motion equations along with the VSAB condition in the time domain is studied. Finally, numerical examples of some classical elastic wave motion problems are presented and the results are compared with the associated theoretical solutions, demonstrating that high precision and adequate stability can be achieved by using the proposed 3D VSAB. The proposed 3D VSAB can be conveniently incorporated in the general finite element program, which is commonly used to study dynamic soil-structure interaction problems.
文摘Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of proteins in various organisms. It contains six predictors: Euk-mPLoc 2.0, Hum-mPLoc 2.0, Plant-mPLoc, Gpos-mPLoc, Gneg-mPLoc, and Virus-mPLoc, specialized for eukaryotic, human, plant, Gram- positive bacterial, Gram-negative bacterial, and virus proteins, respectively. Compared with Cell-PLoc, the predictors in the Cell-PLoc 2.0 have the following advantageous features: (1) they all have the capacity to deal with the multiplex proteins that can simultaneiously exist, or move between, two or more subcellular location sites;(2) no accession number is needed for the input of a query protein even if using the “high- level” GO (gene ontology) prediction engine;(3) the functional domain information and sequential evolution information are fused into the “ab initio” sequence-based prediction engine to enhance its accuracy. In this protocol, a step- to-step guide is provided for how to use the web server predictors in the Cell-PLoc 2.0 package, which is freely accessible to the public at http://www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc-2/.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39770392) the "973" Na- tional Key Basic Research Project (Grant No. G2000057004).
文摘Sex determining gene Mab-3 of C. elegans and doublesex of Drosophila contain a common DNA binding motif called a DM domain, both of which regulate similar aspects of sexual development. Human doublesex-related gene DMRT1 has been identified, which also contains the conserved DM-related DNA-binding domain and plays an essential role in gonadal differentiation. We present the amplification of a broad spectrum of DM domain sequences from phylogenetic diverse vertebrates (Cynops orientalis, Chrysemys scripta elegans and Coturnix coturnix) using degenerate PCR. Our results further reveal the unexpected complexity and the evolutionary conservation of the DM domain gene family.
基金the State Key Basic Research Development Program of China (Grant No. G1999011703) the National Natural Science Foundation of China (Grant No. 39770167).
文摘The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.
基金supported by the NIH grants(R01AI137472 and R01AI139092)intramural funds of the New York Blood Center(VIM-NYB616 and CFM-NYB595).
文摘The outbreak of Coronavirus Disease 2019(COVID-19)has posed a serious threat to global public health,calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),also known as 2019 novel coronavirus(2019-nCoV).The CoV spike(S)protein plays the most important roles in viral attachment,fusion and entry,and serves as a target for development of antibodies,entry inhibitors and vaccines.Here,we identified the receptor-binding domain(RBD)in SARS-CoV-2 S protein and found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2(ACE2)receptors.SARS-CoV-2 RBD exhibited significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD and could block the binding and,hence,attachment of SARS-CoV-2 RBD and SARS-CoV RBD to ACE2-expressing cells,thus inhibiting their infection to host cells.SARS-CoV RBD-specific antibodies could crossreact with SARS-CoV-2 RBD protein,and SARS-CoV RBD-induced antisera could cross-neutralize SARS-CoV-2,suggesting the potential to develop SARS-CoV RBD-based vaccines for prevention of SARS-CoV-2 and SARS-CoV infection.
