目的:评估雾化吸入给药对重组人干扰素-α2b(rhIFN-α2b)生物活性和分子结构的影响。方法:采用病毒抑制法检测rhIFN-α2b的生物活性,通过比较雾化前后rhIFN-α2b生物活性的变化分析空气射流对其生物活性的影响。采用高效液相色谱(HPLC)...目的:评估雾化吸入给药对重组人干扰素-α2b(rhIFN-α2b)生物活性和分子结构的影响。方法:采用病毒抑制法检测rhIFN-α2b的生物活性,通过比较雾化前后rhIFN-α2b生物活性的变化分析空气射流对其生物活性的影响。采用高效液相色谱(HPLC)法和十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析干扰素分子结构的改变。结果:雾化给药不会造成rhIFN-α2b蛋白质肽键断裂,但是空气射流会造成二硫键断裂,断裂比例与雾化前rhIFN-α2b浓度有关,600万IU/m L、300万IU/m L和100万IU/m L rhIFN-α2b雾化后纯度分别为82.9%、90.1%和94.9%。600万IU/m L rhIFN-α2b溶液雾化后17.1%的rhIFN-α2b分子出现二硫键的断裂,其中0.5%的rhIFN-α2b分子出现双二硫键断裂,雾化后喷雾壶内未完全汽化的剩余液中rhIFN-α2b的生物活性保留率为98.2%,雾化后收集的凝集液中rhIFN-α2b的生物活性保留率为96.1%。结论:rhIFN-α2b能以雾化状态稳定传输给药,生物活性基本保留。雾化后绝大部分rhIFN-α2b分子结构保持完整,少数分子出现单个或双二硫键断裂,为了尽量减少rhIFN-α2b分子的损耗,临床上雾化吸入rhIFN-α2b的浓度不应超过100万IU/m L。展开更多
With the prompt development in intellectualization nowadays, the smart materials with multifunctionality or multi-responsiveness are highly expected. But it is a big challenge to integrate the different actuating unit...With the prompt development in intellectualization nowadays, the smart materials with multifunctionality or multi-responsiveness are highly expected. But it is a big challenge to integrate the different actuating units into a single system in a synergy pattern. Herein, we put forward a new strategy to develop the polyurethane networks which can present shape-memory effect and self-healing effect in independent way as well as simultaneous acting mode. To realize this goal, poly(tetremethylene ether) glycol was chosen as the soft segment to ensure the polymer chains a good mobility, and disulfide bond as the dynamic covalent bond was embedded in the backbone of polyurethane to endow it with desirable self-healing capacity under mild condition. Moreover, a rational control of the architecture of the networks by adjusting the content of disulfide bond and the degree of cross-linking, a broad glass transition temperature(T_g) was achieved, which enabled the network a versatile shape-memory effect, covering from dual-, triple-so far as to quadrupleshape memory effect. More importantly, the shape recovery and healing process can be realized simultaneously because of the highly matched actuating condition in this system.展开更多
文摘目的:评估雾化吸入给药对重组人干扰素-α2b(rhIFN-α2b)生物活性和分子结构的影响。方法:采用病毒抑制法检测rhIFN-α2b的生物活性,通过比较雾化前后rhIFN-α2b生物活性的变化分析空气射流对其生物活性的影响。采用高效液相色谱(HPLC)法和十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析干扰素分子结构的改变。结果:雾化给药不会造成rhIFN-α2b蛋白质肽键断裂,但是空气射流会造成二硫键断裂,断裂比例与雾化前rhIFN-α2b浓度有关,600万IU/m L、300万IU/m L和100万IU/m L rhIFN-α2b雾化后纯度分别为82.9%、90.1%和94.9%。600万IU/m L rhIFN-α2b溶液雾化后17.1%的rhIFN-α2b分子出现二硫键的断裂,其中0.5%的rhIFN-α2b分子出现双二硫键断裂,雾化后喷雾壶内未完全汽化的剩余液中rhIFN-α2b的生物活性保留率为98.2%,雾化后收集的凝集液中rhIFN-α2b的生物活性保留率为96.1%。结论:rhIFN-α2b能以雾化状态稳定传输给药,生物活性基本保留。雾化后绝大部分rhIFN-α2b分子结构保持完整,少数分子出现单个或双二硫键断裂,为了尽量减少rhIFN-α2b分子的损耗,临床上雾化吸入rhIFN-α2b的浓度不应超过100万IU/m L。
基金supported financially by the National Natural Science Foundation of China (51773131 and 51721091)the International S&T Cooperation Project of Sichuan Province (2017HH0034)
文摘With the prompt development in intellectualization nowadays, the smart materials with multifunctionality or multi-responsiveness are highly expected. But it is a big challenge to integrate the different actuating units into a single system in a synergy pattern. Herein, we put forward a new strategy to develop the polyurethane networks which can present shape-memory effect and self-healing effect in independent way as well as simultaneous acting mode. To realize this goal, poly(tetremethylene ether) glycol was chosen as the soft segment to ensure the polymer chains a good mobility, and disulfide bond as the dynamic covalent bond was embedded in the backbone of polyurethane to endow it with desirable self-healing capacity under mild condition. Moreover, a rational control of the architecture of the networks by adjusting the content of disulfide bond and the degree of cross-linking, a broad glass transition temperature(T_g) was achieved, which enabled the network a versatile shape-memory effect, covering from dual-, triple-so far as to quadrupleshape memory effect. More importantly, the shape recovery and healing process can be realized simultaneously because of the highly matched actuating condition in this system.
