The growth and activity of photosynthetic CO2 uptake and extracellular carbonic anhydrase (CAext) of the marine diatom Skeletonema costatum were investigated while cultured at different levels of CO2 in order to see i...The growth and activity of photosynthetic CO2 uptake and extracellular carbonic anhydrase (CAext) of the marine diatom Skeletonema costatum were investigated while cultured at different levels of CO2 in order to see its physio-logical response to different CO2 concentrations under either a low (30 靘ol·m-2·s-1) or high (210 靘ol·m-2·s-1) irradiance. The changes in CO2 concentrations (4—31 靘ol/L) affected the growth and net photosynthesis to a greater extent under the low than under the high light re-gime. CAext was detected in the cells grown at 4 mol/L CO2 but not at 31 and 12 靘ol/L CO2, with its activity being about 2.5-fold higher at the high than at the low irradiance. Photo- synthetic CO2 affinity (1/ K1/2(CO2)) of the cells de-creased with increased CO2 concentrations in culture. The cells cultured under the high-light show significantly higher photosynthetic CO2 affinity than those grown at the low-light level. It is concluded that the regulations of CAext activity and photosynthetic CO2 affinity are dependent not only on CO2 concentration but also on light availability, and that the de-velopment of higher CAext activity and CO2 affinity under higher light level could sufficiently support the photosyn-thetic demand for CO2 even at low level of CO2.展开更多
河流拦截筑坝形成蓄水河流,逐渐向“湖泊型”生态系统演化,加强了生物地球化学循环并进一步影响水体碳循环.为了更准确地进行全球碳循环的预算并预测碳循环变化,必须确定对河流系统产生影响的碳来源.因此,通过测定库区水体c(DIC)(DIC为...河流拦截筑坝形成蓄水河流,逐渐向“湖泊型”生态系统演化,加强了生物地球化学循环并进一步影响水体碳循环.为了更准确地进行全球碳循环的预算并预测碳循环变化,必须确定对河流系统产生影响的碳来源.因此,通过测定库区水体c(DIC)(DIC为溶解性无机碳)及其δ13 C(稳定碳同位素),分析了DIC的主要来源及其影响因素.结果表明:①水体c(DIC)为1.80~5.02 mmol/L,而δ13 C DIC(溶解性无机碳的稳定碳同位素)为-7.45‰^-1.26‰.c(DIC)与EC(电导率)、TA(总碱度)均呈正相关,与水温呈负相关.表水层δ13 C DIC与c(DIC)、TA均呈正相关,与EC在入库河流处呈负相关;而滞水层δ13 C DIC与EC、p CO 2(二氧化碳分压)、TA、c(DIC)均呈正相关.②水平方向上,表水层各指标变化明显,TA、EC、SIc(方解石饱和指数)和c(DIC)整体上呈降低趋势,δ13 C DIC从上游至下游逐渐偏正,受碳酸盐矿物溶解影响显著;垂直方向上,热分层和化学分层现象对水的碳循环产生了显著影响.有机质分解在深水层释放大量CO 2致使c(DIC)、p CO 2逐渐升高及δ13 C DIC逐渐降低,c(DIC)及其δ13 C在整个水柱上存在显著的空间异质性.研究显示,光照水库DIC的来源主要有两种,即生物源的土壤CO 2和有机物呼吸产生的溶解CO 2形式的DIC源、碳酸盐矿物风化所产生的碳酸氢盐形式的DIC源.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.39830060 and 30070582)the Natural Science Foundation and Higher Education Ofice of Guangdong Province.
文摘The growth and activity of photosynthetic CO2 uptake and extracellular carbonic anhydrase (CAext) of the marine diatom Skeletonema costatum were investigated while cultured at different levels of CO2 in order to see its physio-logical response to different CO2 concentrations under either a low (30 靘ol·m-2·s-1) or high (210 靘ol·m-2·s-1) irradiance. The changes in CO2 concentrations (4—31 靘ol/L) affected the growth and net photosynthesis to a greater extent under the low than under the high light re-gime. CAext was detected in the cells grown at 4 mol/L CO2 but not at 31 and 12 靘ol/L CO2, with its activity being about 2.5-fold higher at the high than at the low irradiance. Photo- synthetic CO2 affinity (1/ K1/2(CO2)) of the cells de-creased with increased CO2 concentrations in culture. The cells cultured under the high-light show significantly higher photosynthetic CO2 affinity than those grown at the low-light level. It is concluded that the regulations of CAext activity and photosynthetic CO2 affinity are dependent not only on CO2 concentration but also on light availability, and that the de-velopment of higher CAext activity and CO2 affinity under higher light level could sufficiently support the photosyn-thetic demand for CO2 even at low level of CO2.
文摘河流拦截筑坝形成蓄水河流,逐渐向“湖泊型”生态系统演化,加强了生物地球化学循环并进一步影响水体碳循环.为了更准确地进行全球碳循环的预算并预测碳循环变化,必须确定对河流系统产生影响的碳来源.因此,通过测定库区水体c(DIC)(DIC为溶解性无机碳)及其δ13 C(稳定碳同位素),分析了DIC的主要来源及其影响因素.结果表明:①水体c(DIC)为1.80~5.02 mmol/L,而δ13 C DIC(溶解性无机碳的稳定碳同位素)为-7.45‰^-1.26‰.c(DIC)与EC(电导率)、TA(总碱度)均呈正相关,与水温呈负相关.表水层δ13 C DIC与c(DIC)、TA均呈正相关,与EC在入库河流处呈负相关;而滞水层δ13 C DIC与EC、p CO 2(二氧化碳分压)、TA、c(DIC)均呈正相关.②水平方向上,表水层各指标变化明显,TA、EC、SIc(方解石饱和指数)和c(DIC)整体上呈降低趋势,δ13 C DIC从上游至下游逐渐偏正,受碳酸盐矿物溶解影响显著;垂直方向上,热分层和化学分层现象对水的碳循环产生了显著影响.有机质分解在深水层释放大量CO 2致使c(DIC)、p CO 2逐渐升高及δ13 C DIC逐渐降低,c(DIC)及其δ13 C在整个水柱上存在显著的空间异质性.研究显示,光照水库DIC的来源主要有两种,即生物源的土壤CO 2和有机物呼吸产生的溶解CO 2形式的DIC源、碳酸盐矿物风化所产生的碳酸氢盐形式的DIC源.
