目的:建立测定吉他霉素效价的方法。方法:采用比浊法和管碟法对吉他霉素的效价进行测定并比较。结果:吉他霉素浓度在0.8～2.4 u·mL^(-1)的范围内,浓度的对数与吸收度成良好的线性关系,回归方程:A=608.1-725.7 lg C(r=0.9995),平均...目的:建立测定吉他霉素效价的方法。方法:采用比浊法和管碟法对吉他霉素的效价进行测定并比较。结果:吉他霉素浓度在0.8～2.4 u·mL^(-1)的范围内,浓度的对数与吸收度成良好的线性关系,回归方程:A=608.1-725.7 lg C(r=0.9995),平均回收率为100.9%。结论:微生物浊度法测定吉他霉素的效价简便、精确、快速。展开更多
Diffusion of colloidal particles in microchannels has been extensively investigated,where the channel wall is either a no-slip or a slip-passive boundary.However,in the context of active fluids,driving boundary walls ...Diffusion of colloidal particles in microchannels has been extensively investigated,where the channel wall is either a no-slip or a slip-passive boundary.However,in the context of active fluids,driving boundary walls are ubiquitous and are expected to have a substantial effect on the particle dynamics.By mesoscale simulations,we study the diffusion of a chemically active colloidal particle in composite channels,which are constructed by alternately arranging the no-slip and diffusio-osmotic boundary walls.In this case,the chemical reaction catalyzed by the active colloidal particle creates a local chemical gradient along the channel wall,which drives a diffusio-osmotic flow parallel to the wall.We show that the diffusio-osmotic flow can significantly change the spatial distribution and diffusion dynamics of the colloidal particle in the composite channels.By modulating the surface properties of the channel wall,we can achieve different patterns of colloidal position distribution.The findings thus propose a novel possibility to manipulate colloidal diffusion in microfluidics,and highlight the importance of driving boundary walls in dynamics of colloidal particles in microchannels.展开更多
文摘目的:建立测定吉他霉素效价的方法。方法:采用比浊法和管碟法对吉他霉素的效价进行测定并比较。结果:吉他霉素浓度在0.8～2.4 u·mL^(-1)的范围内,浓度的对数与吸收度成良好的线性关系,回归方程:A=608.1-725.7 lg C(r=0.9995),平均回收率为100.9%。结论:微生物浊度法测定吉他霉素的效价简便、精确、快速。
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11874397,11674365,and 11774393)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB33000000)。
文摘Diffusion of colloidal particles in microchannels has been extensively investigated,where the channel wall is either a no-slip or a slip-passive boundary.However,in the context of active fluids,driving boundary walls are ubiquitous and are expected to have a substantial effect on the particle dynamics.By mesoscale simulations,we study the diffusion of a chemically active colloidal particle in composite channels,which are constructed by alternately arranging the no-slip and diffusio-osmotic boundary walls.In this case,the chemical reaction catalyzed by the active colloidal particle creates a local chemical gradient along the channel wall,which drives a diffusio-osmotic flow parallel to the wall.We show that the diffusio-osmotic flow can significantly change the spatial distribution and diffusion dynamics of the colloidal particle in the composite channels.By modulating the surface properties of the channel wall,we can achieve different patterns of colloidal position distribution.The findings thus propose a novel possibility to manipulate colloidal diffusion in microfluidics,and highlight the importance of driving boundary walls in dynamics of colloidal particles in microchannels.
