BACKGROUND The incidence rate of cerebral infarction in young people is increasing day by day,the age of onset tends to be younger,and its internal pathogenesis and mechanism are very complicated,which leads to greate...BACKGROUND The incidence rate of cerebral infarction in young people is increasing day by day,the age of onset tends to be younger,and its internal pathogenesis and mechanism are very complicated,which leads to greater difficulties in treatment.Therefore,it is essential to analyze the key pathway that affects the onset of cerebral infarction in young people from the perspective of genetics.AIM To compare the differentially expressed genes in the brain tissue of young and aged rats with middle cerebral artery occlusion and to analyse their effect on the key signalling pathway involved in the development of cerebral ischaemia in young rats.METHODS The Gene Expression Omnibus 2R online analysis tool was used to analyse the differentially expressed genes in the GSE166162 dataset regarding the development of cerebral ischaemia in young and aged groups of rats.DAVID 6.8 software was further used to filter the differentially expressed genes.These genes were subjected to Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to determine the key gene pathway that affects the occurrence of cerebral ischaemia in young rats.RESULTS Thirty-five differentially expressed genes(such as Igf2,Col1a2,and Sfrp1)were obtained;73 GO enrichment analysis pathways are mainly involved in biological processes such as drug response,amino acid stimulation response,blood vessel development,various signalling pathways,and enzyme regulation.They are involved in molecular functions such as drug binding,protein binding,dopamine binding,metal ion binding,and dopamine neurotransmitter receptor activity.KEGG pathway enrichment analysis showed a significantly enriched pathway:The cyclic adenosine monophosphate(c-AMP)signalling pathway.CONCLUSION The c-AMP signalling pathway might be the key pathway in the intervention of cerebral infarction in young people.展开更多
Based on the exploration and development practice of marine shale gas in Fuling, Weiyuan, Changning, Luzhou and Southeast Chongqing in southern China, combined with experiments and analysis, six factors controlling di...Based on the exploration and development practice of marine shale gas in Fuling, Weiyuan, Changning, Luzhou and Southeast Chongqing in southern China, combined with experiments and analysis, six factors controlling differential enrichment of marine shale gas are summarized as follows:(1) The more appropriate thermal evolution and the higher the abundance of organic matter, the higher the adsorption and total gas content of shale will be.(2) Kerogen pyrolysis and liquid hydrocarbon cracking provide most of the marine shale gas.(3) The specific surface area and pore volume of organic matter rich shale increased first and then decreased with the increase of thermal evolution degree of organic shale. At Ro between 2.23% and 3.33%, the shale reservoirs are mainly oil-wet, which is conducive to the enrichment of shale gas.(4) The thicker the roof and floor, the higher the shale gas content. The longer the last tectonic uplift time and the greater the uplift amplitude, the greater the loss of shale gas will be.(5) The buried depth and dip angle of the stratum have different controlling and coupling effects on shale gas in different tectonic positions, resulting in two differential enrichment models of shale gas.(6) The effective and comprehensive matching of source, reservoir and preservation conditions determines the quality of shale gas accumulation. Good match of effective gas generating amount and time, moderate pore evolution and good preservation conditions in space and time is essential for the enrichment of shale gas.展开更多
文摘BACKGROUND The incidence rate of cerebral infarction in young people is increasing day by day,the age of onset tends to be younger,and its internal pathogenesis and mechanism are very complicated,which leads to greater difficulties in treatment.Therefore,it is essential to analyze the key pathway that affects the onset of cerebral infarction in young people from the perspective of genetics.AIM To compare the differentially expressed genes in the brain tissue of young and aged rats with middle cerebral artery occlusion and to analyse their effect on the key signalling pathway involved in the development of cerebral ischaemia in young rats.METHODS The Gene Expression Omnibus 2R online analysis tool was used to analyse the differentially expressed genes in the GSE166162 dataset regarding the development of cerebral ischaemia in young and aged groups of rats.DAVID 6.8 software was further used to filter the differentially expressed genes.These genes were subjected to Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to determine the key gene pathway that affects the occurrence of cerebral ischaemia in young rats.RESULTS Thirty-five differentially expressed genes(such as Igf2,Col1a2,and Sfrp1)were obtained;73 GO enrichment analysis pathways are mainly involved in biological processes such as drug response,amino acid stimulation response,blood vessel development,various signalling pathways,and enzyme regulation.They are involved in molecular functions such as drug binding,protein binding,dopamine binding,metal ion binding,and dopamine neurotransmitter receptor activity.KEGG pathway enrichment analysis showed a significantly enriched pathway:The cyclic adenosine monophosphate(c-AMP)signalling pathway.CONCLUSION The c-AMP signalling pathway might be the key pathway in the intervention of cerebral infarction in young people.
基金Supported by the China National Science and Technology Major Project(2017ZX05035002)the National Natural Science Foundation of China(41872135,41802153)
文摘Based on the exploration and development practice of marine shale gas in Fuling, Weiyuan, Changning, Luzhou and Southeast Chongqing in southern China, combined with experiments and analysis, six factors controlling differential enrichment of marine shale gas are summarized as follows:(1) The more appropriate thermal evolution and the higher the abundance of organic matter, the higher the adsorption and total gas content of shale will be.(2) Kerogen pyrolysis and liquid hydrocarbon cracking provide most of the marine shale gas.(3) The specific surface area and pore volume of organic matter rich shale increased first and then decreased with the increase of thermal evolution degree of organic shale. At Ro between 2.23% and 3.33%, the shale reservoirs are mainly oil-wet, which is conducive to the enrichment of shale gas.(4) The thicker the roof and floor, the higher the shale gas content. The longer the last tectonic uplift time and the greater the uplift amplitude, the greater the loss of shale gas will be.(5) The buried depth and dip angle of the stratum have different controlling and coupling effects on shale gas in different tectonic positions, resulting in two differential enrichment models of shale gas.(6) The effective and comprehensive matching of source, reservoir and preservation conditions determines the quality of shale gas accumulation. Good match of effective gas generating amount and time, moderate pore evolution and good preservation conditions in space and time is essential for the enrichment of shale gas.
