传统离线数据分析方法对于处理即时性高和流量大的数据存在缺陷,而在线检测模型可以满足数据流分析的实时性要求。文中提出了一种基于多阈值模板的在线检测方法。该方法结合多路搜索树突变点检测(Ternary Search Tree and Kolmogorov-Sm...传统离线数据分析方法对于处理即时性高和流量大的数据存在缺陷,而在线检测模型可以满足数据流分析的实时性要求。文中提出了一种基于多阈值模板的在线检测方法。该方法结合多路搜索树突变点检测(Ternary Search Tree and Kolmogorov-Smirnov,TSTKS)算法进行在线检测,基于突变点密度更新窗口长度从而提高了突变点检测精度。采用等量分级策略实现对时序数据的自学习、匹配和分类,进而对大规模病变数据进行状态检测和预测。仿真实验和病变数据的实验结果表明,所提方法具有效果高、分类准确等优点,为大规模时序数据进行快速分类研究提供了新方法。展开更多
An efficient method was developed to detect point mutations using oligonucleotide microarrays and the ligase detection reaction (LDR). Allele-specific LDR primers were immobilized on polylysine-coated glass slides to ...An efficient method was developed to detect point mutations using oligonucleotide microarrays and the ligase detection reaction (LDR). Allele-specific LDR primers were immobilized on polylysine-coated glass slides to perform LDR on a chip. The spotting concentration and detection limit were analyzed using a synthesized oligonucleotide as a template. The optimal primer spotting concentration was 20 mmol/L and the lowest detectable template concentration was 0.33 nmol/L. The method was successfully employed to iden-tify malignant mutations of hypertrophic cardiomyopathy. Asymmetric polymerase chain reaction was em-ployed to prepare single stranded DNA as LDR templates from cloned plasmids. The discrimination ratios for AC, TC, GT, TT, GA, and AA mismatches are 32.82, 44.24, 17.75, 18.34, 11.66, and 8.91, respectively. This method may allow construction of multiple mutation detection systems.展开更多
文摘传统离线数据分析方法对于处理即时性高和流量大的数据存在缺陷,而在线检测模型可以满足数据流分析的实时性要求。文中提出了一种基于多阈值模板的在线检测方法。该方法结合多路搜索树突变点检测(Ternary Search Tree and Kolmogorov-Smirnov,TSTKS)算法进行在线检测,基于突变点密度更新窗口长度从而提高了突变点检测精度。采用等量分级策略实现对时序数据的自学习、匹配和分类,进而对大规模病变数据进行状态检测和预测。仿真实验和病变数据的实验结果表明,所提方法具有效果高、分类准确等优点,为大规模时序数据进行快速分类研究提供了新方法。
基金the National High-Tech Research and Devel-opment (863) Program of China (No. 2002AA2Z2011)
文摘An efficient method was developed to detect point mutations using oligonucleotide microarrays and the ligase detection reaction (LDR). Allele-specific LDR primers were immobilized on polylysine-coated glass slides to perform LDR on a chip. The spotting concentration and detection limit were analyzed using a synthesized oligonucleotide as a template. The optimal primer spotting concentration was 20 mmol/L and the lowest detectable template concentration was 0.33 nmol/L. The method was successfully employed to iden-tify malignant mutations of hypertrophic cardiomyopathy. Asymmetric polymerase chain reaction was em-ployed to prepare single stranded DNA as LDR templates from cloned plasmids. The discrimination ratios for AC, TC, GT, TT, GA, and AA mismatches are 32.82, 44.24, 17.75, 18.34, 11.66, and 8.91, respectively. This method may allow construction of multiple mutation detection systems.
