Ammonia (NH3) volatilization, denitrification loss, and nitrous oxide (N2O) emission were investigated from an irrigated wheat-maize rotation field on the North China Plain, and the magnitude of gaseous N loss from de...Ammonia (NH3) volatilization, denitrification loss, and nitrous oxide (N2O) emission were investigated from an irrigated wheat-maize rotation field on the North China Plain, and the magnitude of gaseous N loss from denitrification and NH3 volatilization was assessed. The micrometeorological gradient diffusion method in conjunction with a Bowen Ratio system was utilized to measure actual NH3 fluxes over a large area, while the acetylene inhibition technique (intact soil cores) was employed for measurement of denitrification losses and N2O emissions. Ammonia volatilization loss was 26.62% of the applied fertilizer nitrogen (N) under maize, while 0.90% and 15.55% were lost from the wheat field at sowing and topdressing, respectively. The differences in NH3 volatilization between different measurement events may be due to differences between the fertilization methods, and to differences in climatic conditions such as soil temperature.Denitrification losses in the fertilized plots were 0.67%-2.87% and 0.31%-0.49% of the applied fertilizer N under maize and wheat after subtracting those of the controls, respectively. Nitrous oxide emissions in the fertilized plots were approximately 0.08%-0.41% and 0.26%-0.34% of the applied fertilizer N over the maize and wheat seasons after subtracting those of the controls, correspondingly. The fertilizer N losses due to NH3 volatilization were markedly higher than those through denitrification and nitrous oxide emissions. These results indicated that NH3 volatilization was an important N transformation in the crop-soil system and was likely to be the major cause of low efficiencies with N fertilizer in the study area. Denitrification was not a very important pathway of N fertilizer loss, but did result in important evolution of the greenhouse gas N2O and the effect of N2O emitted from agricultural fields on environment should not be overlooked.展开更多
A field experiment was conducted on Chinese cabbage （Brassica campestris L. ssp. pekinensis （Lour.） Olsson） in a Nanjing suburb in 2003. The experiment included 4 treatments in a randomized complete block design w...A field experiment was conducted on Chinese cabbage （Brassica campestris L. ssp. pekinensis （Lour.） Olsson） in a Nanjing suburb in 2003. The experiment included 4 treatments in a randomized complete block design with 3 replicates： zero chemical fertilizer N （CK）; urea at rates of 300 kg N ha^-1 （U300） and 600 kg N ha^-1 （U600）, both as basal and two topdressings; and polymer-coated urea at a rate of 180 kg N ha^-1 （PCU180） as a basal application. The acetylene inhibition technique was used to measure denitrification （N2 ＋ N2O） from intact soil cores and N2O emissions in the absence of acetylene. Results showed that compared to （3K total denitrification losses were significantly greater （P ≤ 0.05） in the PCU180, U300, and U600 treatments,while N2O emissions in the U300 and U600 treatments were significantly higher （P ≤ 0.05） than （3K. In the U300 and U600 treatments peaks of denitrification and N2O emission were usually observed after N application. In the polymer-coated urea treatment （PCU180） during the period 20 to 40 days after transplanting, higher denitrification rates and N2O fluxes occurred. Compared with urea, polymer-coated urea did not show any effect on reducing denitrification losses and N2O emissions in terms of percentage of applied N. As temperature gradually decreased from transplanting to harvest, denitrification rates and N2O emissions tended to decrease. A significant （P ≤0.01） positive correlation occurred between denitrification （r = 0.872） or N2O emission （r = 0.781） flux densities and soil temperature in the CK treatment with a stable nitrate content during the whole growing season.展开更多
Nitrogen (N) losses from ammonium bicarbonate or urea applied to wheat and then followed immediately by irrigation were investigated. Ammonia volatilization was determined by a micrometeorological method (ammonia samp...Nitrogen (N) losses from ammonium bicarbonate or urea applied to wheat and then followed immediately by irrigation were investigated. Ammonia volatilization was determined by a micrometeorological method (ammonia sampler), total N loss was estimated by the 15N mass balance method, and denitrification loss was measured by the difference method (calculated from the difference between the total N loss and ammonia loss)and a direct method (measuring the emission of (N2+N2O)-15N ). Total ammonia losses from ammonium bicarbonate and urea in 33 days were 8.7% and 0.9% of the applied nitrogen, respectively. The corresponding total N losses were 21.6% and 29.5%. Apparent denitrification losses (by the difference method) were rather high, being 12.9% from ammonium bicarbonate and 28.6% from urea. However, no emission of (N2+N2O)-15N was detected using the direct method.展开更多
采用原状土柱-乙炔抑制培养法研究了施肥对紫色土玉米生长季土壤N2O排放通量和反硝化作用的影响。结果表明:玉米季施肥显著增加土壤N2O排放和反硝化损失,同时,各施肥处理间N2O排放与反硝化损失量差异显著。猪厩肥、猪厩肥配施氮磷钾肥...采用原状土柱-乙炔抑制培养法研究了施肥对紫色土玉米生长季土壤N2O排放通量和反硝化作用的影响。结果表明:玉米季施肥显著增加土壤N2O排放和反硝化损失,同时,各施肥处理间N2O排放与反硝化损失量差异显著。猪厩肥、猪厩肥配施氮磷钾肥、氮肥、氮磷钾肥和秸秆配施氮磷钾肥等处理的土壤N2O排放量分别为3.01、2.86、2.51、2.19和1.88 kg hm-2,分别占当季氮肥施用量的1.63%、1.53%、1.30%、1.09%和0.88%,反硝化损失量分别为6.74、6.11、5.23、4.69和4.12 kg hm-2,分别占当季氮肥施用量的3.97%、3.55%、2.97%、2.61%和2.23%,不施肥土壤的N2O排放量和反硝化损失量仅为0.56和0.78 kg hm-2。施肥是紫色土玉米生长前期(2周内)土壤N2O排放和反硝化速率出现高峰的主要驱动因子,土壤铵态氮和硝态氮含量是影响土壤N2O排放、土壤硝化和反硝化作用的限制因子,土壤含水量是重要影响因子,降雨是主要促发因素。土壤N2O排放量与反硝化损失量的比值介于0.45～0.72之间,土壤反硝化损失量极显著高于土壤N2O排放量,说明土壤反硝化作用是紫色土玉米生长季氮肥损失的重要途径。展开更多
Denitrification losses measured by direct method (measuring the evolution of (N2+N2O)-15N) were compared with the apparent denitrification losses (calculated from the difference between the total N loss and ammonia lo...Denitrification losses measured by direct method (measuring the evolution of (N2+N2O)-15N) were compared with the apparent denitrification losses (calculated from the difference between the total N loss and ammonia loss), for fertilizers applied to flooded soils. The direct measured denitrification losses from potassium nitrate were 23.0%, 40.0%, and 63.1-79.7% of applied N in rice field, and in incubations of 7 cm deep layer of soil and 2 cm deep layer of soil, respectively; while the corresponding apparent denitrification losses were 96.0%, 98.4%, and 97.7-97.9%, respectively. In field experiments with urea, the direct measured denitrification losses ranged from 0.1-1.8%, which were much less than the apparent denitrification losses (41.3-45.7%). Such discrepancies were primarily due to the entrapment of the gaseous products of denitrification in the soil as revealed by the facts: (1) stirring the floodwater and the surface soil markedly increased the fluxes of (N2+N2O)-15N from urea or potassium nitrate applied to the flooded rice field, and (2) reducing the pressure in the headspace of the incubation bottle with the 7 cm soil layer during gas sampling decreased the discrepancy between the direct measured and apparent denitrifecation losses from 58.4% to 21.2%. The advantage of reducing the pressure in the headspace is that there is minimal disturbance of the soil. Further testing of this technique in rice field is needed to determine its effectiveness in releasing the entrapped gaseous products of denitrification so that denitrification losses can be quantified directly.展开更多
Periphytic biofilms are commonly presented at the water-soil interface in paddy fields. Different fertilization methods can affect the concentration and distribution of nutrients in paddy fields and thus affect the de...Periphytic biofilms are commonly presented at the water-soil interface in paddy fields. Different fertilization methods can affect the concentration and distribution of nutrients in paddy fields and thus affect the development of periphytic biofilms. In this study, the roles of periphytic biofilms in nitrogen(N) cycling in paddy systems and how they are affected by different fertilization methods were studied using microcosm experiments. Microcosms were prepared using soil samples from a paddy field and treated with surface and deep fertilization under light and dark conditions. Surface fertilization under light condition promoted the development of periphytic biofilms, while deep fertilization under dark condition inhibited their development. The development of periphytic biofilms increased the pH and dissolved oxygen levels in the overlying water. Surface fertilization resulted in high N concentrations in the overlying water and the topsoil layers, which enhanced NH3 volatilization and nitrification-denitrification but inhibited N fixation. The development of periphytic biofilms reduced NH3 volatilization loss but increased nitrification-denitrification loss and the overall N loss in the paddy system. The results from this work suggest that the presence of periphytic biofilms in paddy fields could increase N loss by 3.10%–7.11%. Deep fertilization is an effective method to retard the development of periphytic biofilms in the paddy system and can potentially increase the overall N use efficiency.展开更多
基金Project supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KZCX2-413-3)the National Key Basic Research Support Foundation (NKBRSF) of China (No. G1999011803) the Australian Centre for
文摘Ammonia (NH3) volatilization, denitrification loss, and nitrous oxide (N2O) emission were investigated from an irrigated wheat-maize rotation field on the North China Plain, and the magnitude of gaseous N loss from denitrification and NH3 volatilization was assessed. The micrometeorological gradient diffusion method in conjunction with a Bowen Ratio system was utilized to measure actual NH3 fluxes over a large area, while the acetylene inhibition technique (intact soil cores) was employed for measurement of denitrification losses and N2O emissions. Ammonia volatilization loss was 26.62% of the applied fertilizer nitrogen (N) under maize, while 0.90% and 15.55% were lost from the wheat field at sowing and topdressing, respectively. The differences in NH3 volatilization between different measurement events may be due to differences between the fertilization methods, and to differences in climatic conditions such as soil temperature.Denitrification losses in the fertilized plots were 0.67%-2.87% and 0.31%-0.49% of the applied fertilizer N under maize and wheat after subtracting those of the controls, respectively. Nitrous oxide emissions in the fertilized plots were approximately 0.08%-0.41% and 0.26%-0.34% of the applied fertilizer N over the maize and wheat seasons after subtracting those of the controls, correspondingly. The fertilizer N losses due to NH3 volatilization were markedly higher than those through denitrification and nitrous oxide emissions. These results indicated that NH3 volatilization was an important N transformation in the crop-soil system and was likely to be the major cause of low efficiencies with N fertilizer in the study area. Denitrification was not a very important pathway of N fertilizer loss, but did result in important evolution of the greenhouse gas N2O and the effect of N2O emitted from agricultural fields on environment should not be overlooked.
基金Project supported by the National Natural Science Foundation of China (No. 40171048)the Science and Technique Key Project of the Tenth Five-Year Plan of China (No. 2002BA516A03)
文摘A field experiment was conducted on Chinese cabbage （Brassica campestris L. ssp. pekinensis （Lour.） Olsson） in a Nanjing suburb in 2003. The experiment included 4 treatments in a randomized complete block design with 3 replicates： zero chemical fertilizer N （CK）; urea at rates of 300 kg N ha^-1 （U300） and 600 kg N ha^-1 （U600）, both as basal and two topdressings; and polymer-coated urea at a rate of 180 kg N ha^-1 （PCU180） as a basal application. The acetylene inhibition technique was used to measure denitrification （N2 ＋ N2O） from intact soil cores and N2O emissions in the absence of acetylene. Results showed that compared to （3K total denitrification losses were significantly greater （P ≤ 0.05） in the PCU180, U300, and U600 treatments,while N2O emissions in the U300 and U600 treatments were significantly higher （P ≤ 0.05） than （3K. In the U300 and U600 treatments peaks of denitrification and N2O emission were usually observed after N application. In the polymer-coated urea treatment （PCU180） during the period 20 to 40 days after transplanting, higher denitrification rates and N2O fluxes occurred. Compared with urea, polymer-coated urea did not show any effect on reducing denitrification losses and N2O emissions in terms of percentage of applied N. As temperature gradually decreased from transplanting to harvest, denitrification rates and N2O emissions tended to decrease. A significant （P ≤0.01） positive correlation occurred between denitrification （r = 0.872） or N2O emission （r = 0.781） flux densities and soil temperature in the CK treatment with a stable nitrate content during the whole growing season.
文摘Nitrogen (N) losses from ammonium bicarbonate or urea applied to wheat and then followed immediately by irrigation were investigated. Ammonia volatilization was determined by a micrometeorological method (ammonia sampler), total N loss was estimated by the 15N mass balance method, and denitrification loss was measured by the difference method (calculated from the difference between the total N loss and ammonia loss)and a direct method (measuring the emission of (N2+N2O)-15N ). Total ammonia losses from ammonium bicarbonate and urea in 33 days were 8.7% and 0.9% of the applied nitrogen, respectively. The corresponding total N losses were 21.6% and 29.5%. Apparent denitrification losses (by the difference method) were rather high, being 12.9% from ammonium bicarbonate and 28.6% from urea. However, no emission of (N2+N2O)-15N was detected using the direct method.
文摘采用原状土柱-乙炔抑制培养法研究了施肥对紫色土玉米生长季土壤N2O排放通量和反硝化作用的影响。结果表明:玉米季施肥显著增加土壤N2O排放和反硝化损失,同时,各施肥处理间N2O排放与反硝化损失量差异显著。猪厩肥、猪厩肥配施氮磷钾肥、氮肥、氮磷钾肥和秸秆配施氮磷钾肥等处理的土壤N2O排放量分别为3.01、2.86、2.51、2.19和1.88 kg hm-2,分别占当季氮肥施用量的1.63%、1.53%、1.30%、1.09%和0.88%,反硝化损失量分别为6.74、6.11、5.23、4.69和4.12 kg hm-2,分别占当季氮肥施用量的3.97%、3.55%、2.97%、2.61%和2.23%,不施肥土壤的N2O排放量和反硝化损失量仅为0.56和0.78 kg hm-2。施肥是紫色土玉米生长前期(2周内)土壤N2O排放和反硝化速率出现高峰的主要驱动因子,土壤铵态氮和硝态氮含量是影响土壤N2O排放、土壤硝化和反硝化作用的限制因子,土壤含水量是重要影响因子,降雨是主要促发因素。土壤N2O排放量与反硝化损失量的比值介于0.45～0.72之间,土壤反硝化损失量极显著高于土壤N2O排放量,说明土壤反硝化作用是紫色土玉米生长季氮肥损失的重要途径。
文摘Denitrification losses measured by direct method (measuring the evolution of (N2+N2O)-15N) were compared with the apparent denitrification losses (calculated from the difference between the total N loss and ammonia loss), for fertilizers applied to flooded soils. The direct measured denitrification losses from potassium nitrate were 23.0%, 40.0%, and 63.1-79.7% of applied N in rice field, and in incubations of 7 cm deep layer of soil and 2 cm deep layer of soil, respectively; while the corresponding apparent denitrification losses were 96.0%, 98.4%, and 97.7-97.9%, respectively. In field experiments with urea, the direct measured denitrification losses ranged from 0.1-1.8%, which were much less than the apparent denitrification losses (41.3-45.7%). Such discrepancies were primarily due to the entrapment of the gaseous products of denitrification in the soil as revealed by the facts: (1) stirring the floodwater and the surface soil markedly increased the fluxes of (N2+N2O)-15N from urea or potassium nitrate applied to the flooded rice field, and (2) reducing the pressure in the headspace of the incubation bottle with the 7 cm soil layer during gas sampling decreased the discrepancy between the direct measured and apparent denitrifecation losses from 58.4% to 21.2%. The advantage of reducing the pressure in the headspace is that there is minimal disturbance of the soil. Further testing of this technique in rice field is needed to determine its effectiveness in releasing the entrapped gaseous products of denitrification so that denitrification losses can be quantified directly.
基金the support from the State Key Development Program for Basic Research of China (No. 2015CB158200)the State Key Laboratory of Freshwater Ecology and Biotechnology of China (No. 2019FBZ03)。
文摘Periphytic biofilms are commonly presented at the water-soil interface in paddy fields. Different fertilization methods can affect the concentration and distribution of nutrients in paddy fields and thus affect the development of periphytic biofilms. In this study, the roles of periphytic biofilms in nitrogen(N) cycling in paddy systems and how they are affected by different fertilization methods were studied using microcosm experiments. Microcosms were prepared using soil samples from a paddy field and treated with surface and deep fertilization under light and dark conditions. Surface fertilization under light condition promoted the development of periphytic biofilms, while deep fertilization under dark condition inhibited their development. The development of periphytic biofilms increased the pH and dissolved oxygen levels in the overlying water. Surface fertilization resulted in high N concentrations in the overlying water and the topsoil layers, which enhanced NH3 volatilization and nitrification-denitrification but inhibited N fixation. The development of periphytic biofilms reduced NH3 volatilization loss but increased nitrification-denitrification loss and the overall N loss in the paddy system. The results from this work suggest that the presence of periphytic biofilms in paddy fields could increase N loss by 3.10%–7.11%. Deep fertilization is an effective method to retard the development of periphytic biofilms in the paddy system and can potentially increase the overall N use efficiency.
