Aims When sympatric flowering plant species in a natural community share pollinators,study of plant-plant interactions via interspecific pollen transfer(iPT)is essential for understanding species coexistence.However,l...Aims When sympatric flowering plant species in a natural community share pollinators,study of plant-plant interactions via interspecific pollen transfer(iPT)is essential for understanding species coexistence.However,little is known about the extent of iPT between interactive species and its causes.Methods To explore how sympatric flowering plants sharing pollinators mini-mize deleterious effects of iPT,we investigated the pollination ecol-ogy of two endemic species,Salvia przewalskii and Delphinium yuanum,in an alpine meadow in the Hengduan mountains,southwest China.We quantified conspecific and interspecific visits by shared bumblebee pollinators,amounts of pollen placed on different body sites of the pollinators and stigmatic pollen loads on open-pollinated flowers.To examine whether iPT affects female fitness,we measured pollen germination and seed production in the two species in an artificial pollination experiment.Important Findingsone bumblebee species,Bombus trifasciatus,was found to be the sole effective pollinator for the two coflowering species.Pollination experiments indicated that deposition of heterospecific pollen could significantly decrease seed set in both species.Experiments showed that S.przewalskii pollen could germinate well on stigmas of D.yuanum,inhibiting conspecific pollen germination in D.yuanum.However,seed set was not lower under open pollination than under cross-pollination within species,suggesting that no female fitness loss was caused by iPT.in foraging bouts with pollinator switches,switches from S.przewalskii to D.yuanum were relatively more fre-quent(8.27%)than the converse(1.72%).However,iPT from S.prze-walskii to D.yuanum accounted for only 1.82%of total stigmatic pollen loads while the reverse iPT to S.przewalskii was 8.70%,indi-cating that more switches of bumblebees to D.yuanum did not result in higher iPT.by contrast,selection for reduced iPT to S.przewalskii would limit pollinator switches from D.yuanum.We found that a bumblebee generally carried pollen grains from both 展开更多
Three new C20-diterpenoid alkaloids, designated as anthriscifolmines A-C （1-3）, together with two known alkaloids denudatine and delgramine, were isolated from the whole herb of Delphinium anthriscifolium var. savat...Three new C20-diterpenoid alkaloids, designated as anthriscifolmines A-C （1-3）, together with two known alkaloids denudatine and delgramine, were isolated from the whole herb of Delphinium anthriscifolium var. savatieri. The structures of these new alkaloids were elucidated on the basis of spectral data.展开更多
Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A （1） and delphatisine B （2）, respectivel...Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A （1） and delphatisine B （2）, respectively. The structures of the new compounds were deduced on the basis of their spectral data （IR, HREIMS, EIMS, 1 D, 2D-NMR）. This is the first report on the isolation of diterpenoid alkaloids from the D. chrysotrichum.展开更多
Two new C19-diterpenoid alkaloids, davidisines A （1） and B （2） along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral meth...Two new C19-diterpenoid alkaloids, davidisines A （1） and B （2） along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral methods, especially 2D NMR techniques.展开更多
The inclusion complex of lappaconitine (Lap) with b-cyclodextrin (b-CD) has been studied by the differential scanning calorimetry (DSC) and 1H-NMR, 2D-NMR spectroscopy. The structure of the complex has been deduced.
Three new C19-diterpenoid alkaloids, giraldines A (1), B (3) and C (4), were isolated from the roots of Delphinium giraldii Diels, together with three known C19-diterpenoid alkaloids, di-hydrogadesine (5), tatsiensine...Three new C19-diterpenoid alkaloids, giraldines A (1), B (3) and C (4), were isolated from the roots of Delphinium giraldii Diels, together with three known C19-diterpenoid alkaloids, di-hydrogadesine (5), tatsiensine (6) and siwanine A (7), as well as their structures were elucidated by chemical evidence and spectral analyses, including IR, MS, 1D NMR and 2D NMR.展开更多
Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagula...Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagulant activity which was extracted with 70%ethanol in our previous researches.Then,16 compounds were isolated and identified from the extract of D.brunonianum,among which compounds 5,7-10,12,14,15-16 were isolated from this genus for the first time,and compounds 2-4 were isolated from this plant for the first time.And the coagulation activity assay showed that compounds 10,14 and 15 had good anticoagulant activity by activated partial thromboplastin time(APTT),thrombin time(TT)and prothrombin time(PT)in vitro.展开更多
目的研究天台山翠雀花Delphinium taiantishanense W.J.Zhang et G.H.Chen中生物碱的化学成分。方法采用酸提碱沉法提取总碱,硅胶层析法分离,波谱法鉴定结构。结果从中分得7个单体化合物。结论应用光谱法鉴定了这些化合物的结构。7个化...目的研究天台山翠雀花Delphinium taiantishanense W.J.Zhang et G.H.Chen中生物碱的化学成分。方法采用酸提碱沉法提取总碱,硅胶层析法分离,波谱法鉴定结构。结果从中分得7个单体化合物。结论应用光谱法鉴定了这些化合物的结构。7个化合物均为已知的C19-二萜生物碱,分别为:6-acetyldelpheline、deltaline、氨茴酰牛扁碱、牛扁碱、德尔色明甲、德尔色明乙和甲基牛扁碱。展开更多
基金National Science Foundation of China(31030016,31270281)to SQ Huang.
文摘Aims When sympatric flowering plant species in a natural community share pollinators,study of plant-plant interactions via interspecific pollen transfer(iPT)is essential for understanding species coexistence.However,little is known about the extent of iPT between interactive species and its causes.Methods To explore how sympatric flowering plants sharing pollinators mini-mize deleterious effects of iPT,we investigated the pollination ecol-ogy of two endemic species,Salvia przewalskii and Delphinium yuanum,in an alpine meadow in the Hengduan mountains,southwest China.We quantified conspecific and interspecific visits by shared bumblebee pollinators,amounts of pollen placed on different body sites of the pollinators and stigmatic pollen loads on open-pollinated flowers.To examine whether iPT affects female fitness,we measured pollen germination and seed production in the two species in an artificial pollination experiment.Important Findingsone bumblebee species,Bombus trifasciatus,was found to be the sole effective pollinator for the two coflowering species.Pollination experiments indicated that deposition of heterospecific pollen could significantly decrease seed set in both species.Experiments showed that S.przewalskii pollen could germinate well on stigmas of D.yuanum,inhibiting conspecific pollen germination in D.yuanum.However,seed set was not lower under open pollination than under cross-pollination within species,suggesting that no female fitness loss was caused by iPT.in foraging bouts with pollinator switches,switches from S.przewalskii to D.yuanum were relatively more fre-quent(8.27%)than the converse(1.72%).However,iPT from S.prze-walskii to D.yuanum accounted for only 1.82%of total stigmatic pollen loads while the reverse iPT to S.przewalskii was 8.70%,indi-cating that more switches of bumblebees to D.yuanum did not result in higher iPT.by contrast,selection for reduced iPT to S.przewalskii would limit pollinator switches from D.yuanum.We found that a bumblebee generally carried pollen grains from both
基金supported by the National Natural Science Foundation of China(No.30472075)the Excellent Ph.D.Dissertation Foundation of China(No.200367)
文摘Three new C20-diterpenoid alkaloids, designated as anthriscifolmines A-C （1-3）, together with two known alkaloids denudatine and delgramine, were isolated from the whole herb of Delphinium anthriscifolium var. savatieri. The structures of these new alkaloids were elucidated on the basis of spectral data.
文摘Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A （1） and delphatisine B （2）, respectively. The structures of the new compounds were deduced on the basis of their spectral data （IR, HREIMS, EIMS, 1 D, 2D-NMR）. This is the first report on the isolation of diterpenoid alkaloids from the D. chrysotrichum.
文摘Two new C19-diterpenoid alkaloids, davidisines A （1） and B （2） along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral methods, especially 2D NMR techniques.
文摘The inclusion complex of lappaconitine (Lap) with b-cyclodextrin (b-CD) has been studied by the differential scanning calorimetry (DSC) and 1H-NMR, 2D-NMR spectroscopy. The structure of the complex has been deduced.
基金Project supported by the Doctoral Foundation of the Ministry of Education,China(2002-2004,No.20010610047).
文摘Three new C19-diterpenoid alkaloids, giraldines A (1), B (3) and C (4), were isolated from the roots of Delphinium giraldii Diels, together with three known C19-diterpenoid alkaloids, di-hydrogadesine (5), tatsiensine (6) and siwanine A (7), as well as their structures were elucidated by chemical evidence and spectral analyses, including IR, MS, 1D NMR and 2D NMR.
基金This work was funded by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagulant activity which was extracted with 70%ethanol in our previous researches.Then,16 compounds were isolated and identified from the extract of D.brunonianum,among which compounds 5,7-10,12,14,15-16 were isolated from this genus for the first time,and compounds 2-4 were isolated from this plant for the first time.And the coagulation activity assay showed that compounds 10,14 and 15 had good anticoagulant activity by activated partial thromboplastin time(APTT),thrombin time(TT)and prothrombin time(PT)in vitro.
文摘目的研究天台山翠雀花Delphinium taiantishanense W.J.Zhang et G.H.Chen中生物碱的化学成分。方法采用酸提碱沉法提取总碱,硅胶层析法分离,波谱法鉴定结构。结果从中分得7个单体化合物。结论应用光谱法鉴定了这些化合物的结构。7个化合物均为已知的C19-二萜生物碱,分别为:6-acetyldelpheline、deltaline、氨茴酰牛扁碱、牛扁碱、德尔色明甲、德尔色明乙和甲基牛扁碱。
