The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Micro...The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Microbial communities were surveyed by denatured gradient gel electrophoresis （DGGE） and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles sepa- rated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, repre- senting two selected hydrothermal samples （19-4TVG8 and 19-4TVG11）, were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hy- drothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.展开更多
西南印度洋中脊(Southwest Indian Ridge,SWIR)热液区具有潜在发育的大规模硫化物矿床,当前正在开展SWIR硫化物矿产资源评价。测量分析硫化物和不同围岩的声速等物性特征是硫化物近底地震勘探资料处理和解释的基础。该文对西南印度洋中...西南印度洋中脊(Southwest Indian Ridge,SWIR)热液区具有潜在发育的大规模硫化物矿床,当前正在开展SWIR硫化物矿产资源评价。测量分析硫化物和不同围岩的声速等物性特征是硫化物近底地震勘探资料处理和解释的基础。该文对西南印度洋中脊热液区的硫化物和围岩等样品进行了系统的物性测量,结合岩石物性(包括密度、孔隙度、P波速度)与矿物组成,深入分析了西南印度洋中脊热液区岩石声速变化特性及其影响因素。结果表明,SWIR热液区围岩的P波速度受到岩石骨架矿物、孔隙和围压的影响。由于岩石孔隙度总体偏小,对P波速度的影响并不显著,但围压的增加使岩石微裂缝和孔隙逐渐闭合,P波速度呈非线性指数变化。蚀变作用导致了矿物成分改变,是影响围岩声速的最关键因素。单一物性参数测量结果可能存在多解性,联合波速、密度、磁性和电性等多物性参数测量有利于岩性区分。该研究成果有助于识别硫化物和围岩,为我国西南印度洋合同区多金属硫化物地震勘探工作提供重要支撑。展开更多
基金Foundation item: The China Ocean Mineral Resources Research and Development Association under contract No. DYXM-115-02-2-07the State Oceanic Administration of People’s Republic of China under contract No. 200805032the National Natural Science Foundation of China under contract Nos 50621063 and 40646029
文摘The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. Microbial communities were surveyed by denatured gradient gel electrophoresis （DGGE） and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles sepa- rated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, repre- senting two selected hydrothermal samples （19-4TVG8 and 19-4TVG11）, were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hy- drothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.
文摘西南印度洋中脊(Southwest Indian Ridge,SWIR)热液区具有潜在发育的大规模硫化物矿床,当前正在开展SWIR硫化物矿产资源评价。测量分析硫化物和不同围岩的声速等物性特征是硫化物近底地震勘探资料处理和解释的基础。该文对西南印度洋中脊热液区的硫化物和围岩等样品进行了系统的物性测量,结合岩石物性(包括密度、孔隙度、P波速度)与矿物组成,深入分析了西南印度洋中脊热液区岩石声速变化特性及其影响因素。结果表明,SWIR热液区围岩的P波速度受到岩石骨架矿物、孔隙和围压的影响。由于岩石孔隙度总体偏小,对P波速度的影响并不显著,但围压的增加使岩石微裂缝和孔隙逐渐闭合,P波速度呈非线性指数变化。蚀变作用导致了矿物成分改变,是影响围岩声速的最关键因素。单一物性参数测量结果可能存在多解性,联合波速、密度、磁性和电性等多物性参数测量有利于岩性区分。该研究成果有助于识别硫化物和围岩,为我国西南印度洋合同区多金属硫化物地震勘探工作提供重要支撑。
