Objective:To study the leaves of Adiantum philippense L.for their antioxidant,cytotoxicity and thrombolytic activities and to perform phytochemical evaluation.Methods:In-vitro antioxidant activity of extract was studi...Objective:To study the leaves of Adiantum philippense L.for their antioxidant,cytotoxicity and thrombolytic activities and to perform phytochemical evaluation.Methods:In-vitro antioxidant activity of extract was studied using DPPH radical scavenging,reducing power,total phenol and total flavonoid content determination assays.The cytotoxic activity was determined using brine shrimp lethality bioassay,thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis.Results:The antioxidant activity of the extracts was found promising.The reducing power of this crude extract increase with the increase of concentration;IC_(50)values of DPPH scavenging activity was(140.00±0.86)μg/mL as compared to ascorbic acid[IC_(50)(130.00±0.76)μg/mL];Total phenol and total flavonoids content were(148.26±0.24)mg/mL and(163.06±0.56)mg/mL respecnvely.In cytotoxicity assay the LC_(50)values of the sample was(106.41±0.78)μg/mL where as for standard vincristin sulphate was(08.50±0.24)μg/mL as a positive control and the extract shows(12.8611.02)%clot lytic whereas standard streptokinase shows(30.86+0.44%clot lytic activity in thrombolytic assay.The phytochemical evaluation indicates the presence of chemical constituents including carbohydrates,alkaloids,saponins,glycosides,flavonoids.Conclusions:This study shows that the methanol extract of leaves of Adiantum philippense L.has bioactivitv but further compound isolation is necessary to confirm the activities of individual compounds.展开更多
A series of samarium(Sm)doped hydroxyapatites containing strontium(Sr)with controllable and adjustable luminescence were designed and prepared via chemical hydrolysis co-precipitation method.The effects of doping conc...A series of samarium(Sm)doped hydroxyapatites containing strontium(Sr)with controllable and adjustable luminescence were designed and prepared via chemical hydrolysis co-precipitation method.The effects of doping concentration of Sm on the structure,composition,photoluminescence(PL),energy transfer,substitute site,fluoresce lifetime and luminescence color of samples were investigated.It is shown that the introduction of Sr ions induces the structure and composition change for Sm doped samples.The typical transitions of Sm^(3+) at 564,601,648 and 707 nm due to^(4)G_(5/2)→^(6)H_(J/2)(J=5,7,9 and 11)transition under the excitation of 404 nm were detected.The PL intensity is affected by the Sr/Ca ratio and Sm concentration,and the optimal concentration of Sm is 0.8 mol%when Sr/Ca ratio equals 7/3.The non-radiative transitions and energy transfer of Sm ions are the main reason for the quenching,and the interactions among ions are ascribed to dipole-dipole type by theoretical calculation.Besides,the ratio of PL intensity between 601 and 648 nm(r_(O/R)=I_(601nm)/I_(648nm))indicates the symmetry and substitution process of Sm ions to Ca/Sr ions.The fluorescence lifetime(τ)decreases with the concentration of Sm increasing,which is the result of the decrease of distance and the enhanced interaction between Sm ions.The CIE chromaticity coordinates and luminescence color of samples are in the orange-red region and have close relation with the concentration of Sm.Furthermore,the cytotoxicity evaluation and confocal laser scanning microscopy(CLSM)observation of samples via cells indicate the good biocompatibility and safe use of this kind of bio-based luminescent materials for their potential efficient fluorescence labeling and detection.展开更多
A series of novel 2H-pyrazolo[4,3-c]hexahydropyridine derivatives （II） have been designed and synthesized. The target com- pounds have been identified by elemental analysis and spectral （1H NMR, IR, and MS） data a...A series of novel 2H-pyrazolo[4,3-c]hexahydropyridine derivatives （II） have been designed and synthesized. The target com- pounds have been identified by elemental analysis and spectral （1H NMR, IR, and MS） data and the absolute configuration of compound （IIl） was confirmed by single crystal X-ray diffraction. The cytotoxicity of the target compounds have been evalu- ated in vitro against two human breast cancer cell lines MCF-7 and MDA-MB-231 by MTT assay. Most compounds exhibited good inhibition, and compounds II21 （IC50 = 4.7 μM for MCF-7 and IC50 = 9.3 μM for MDA-MB-231）, 1133 （IC50 = 2.4 μM for MCF-7 and IC50 = 4.2 gM for MDA-MB-231） and 114o （IC50 = 3.3 μM for MCF-7 and IC5o =8.6 μM for MDA-MB-231） dis- played better inhibitory activity than 5-fluorouracil （IC50 = 4.8 μM for MCF-7 and IC50 = 9.6 I, tM for MDA-MB-231, respec- tively）. Flow cytometric analysis and DNA fragmentation suggest that II33 is cytotoxic and able to induce the apoptosis of MCF-7 cells. The fluorescence properties of compounds IIl, II6, II11,II16, II23, Il2s, and II3s were also studied and compound Ilzs afforded the highest photoluminescence quantum yield （38%）.展开更多
基金Supported by Department of Pharmacy,International Islamic University Chittagong,Bangladesh.Grant No.Pharm-97/06-2012
文摘Objective:To study the leaves of Adiantum philippense L.for their antioxidant,cytotoxicity and thrombolytic activities and to perform phytochemical evaluation.Methods:In-vitro antioxidant activity of extract was studied using DPPH radical scavenging,reducing power,total phenol and total flavonoid content determination assays.The cytotoxic activity was determined using brine shrimp lethality bioassay,thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis.Results:The antioxidant activity of the extracts was found promising.The reducing power of this crude extract increase with the increase of concentration;IC_(50)values of DPPH scavenging activity was(140.00±0.86)μg/mL as compared to ascorbic acid[IC_(50)(130.00±0.76)μg/mL];Total phenol and total flavonoids content were(148.26±0.24)mg/mL and(163.06±0.56)mg/mL respecnvely.In cytotoxicity assay the LC_(50)values of the sample was(106.41±0.78)μg/mL where as for standard vincristin sulphate was(08.50±0.24)μg/mL as a positive control and the extract shows(12.8611.02)%clot lytic whereas standard streptokinase shows(30.86+0.44%clot lytic activity in thrombolytic assay.The phytochemical evaluation indicates the presence of chemical constituents including carbohydrates,alkaloids,saponins,glycosides,flavonoids.Conclusions:This study shows that the methanol extract of leaves of Adiantum philippense L.has bioactivitv but further compound isolation is necessary to confirm the activities of individual compounds.
基金Project supported by the National Natural Science Foundation of China(51972201)International Science and Technology Cooperation of Science and Technology Department of Shaanxi Province of China(2017KW-024)。
文摘A series of samarium(Sm)doped hydroxyapatites containing strontium(Sr)with controllable and adjustable luminescence were designed and prepared via chemical hydrolysis co-precipitation method.The effects of doping concentration of Sm on the structure,composition,photoluminescence(PL),energy transfer,substitute site,fluoresce lifetime and luminescence color of samples were investigated.It is shown that the introduction of Sr ions induces the structure and composition change for Sm doped samples.The typical transitions of Sm^(3+) at 564,601,648 and 707 nm due to^(4)G_(5/2)→^(6)H_(J/2)(J=5,7,9 and 11)transition under the excitation of 404 nm were detected.The PL intensity is affected by the Sr/Ca ratio and Sm concentration,and the optimal concentration of Sm is 0.8 mol%when Sr/Ca ratio equals 7/3.The non-radiative transitions and energy transfer of Sm ions are the main reason for the quenching,and the interactions among ions are ascribed to dipole-dipole type by theoretical calculation.Besides,the ratio of PL intensity between 601 and 648 nm(r_(O/R)=I_(601nm)/I_(648nm))indicates the symmetry and substitution process of Sm ions to Ca/Sr ions.The fluorescence lifetime(τ)decreases with the concentration of Sm increasing,which is the result of the decrease of distance and the enhanced interaction between Sm ions.The CIE chromaticity coordinates and luminescence color of samples are in the orange-red region and have close relation with the concentration of Sm.Furthermore,the cytotoxicity evaluation and confocal laser scanning microscopy(CLSM)observation of samples via cells indicate the good biocompatibility and safe use of this kind of bio-based luminescent materials for their potential efficient fluorescence labeling and detection.
基金supported by Zooblast-molecular Biology Laboratory of Shanghai Normal Universitysupported by the National Natural Science Foundation of China (21042010, 21102092 and30870560)+4 种基金the Key Scientific "Twelfth Five-Year" National Technology Support Program (2011BAE06B01-17)the Innovation Project of Shanghai Education Commission (12YZ078)the Key Project of the Science and Technology Commission of Shanghai 105405503400)the Leading Academic Discipline Project of Shanghai Normal University (DZL808)Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University (07dz22303)
文摘A series of novel 2H-pyrazolo[4,3-c]hexahydropyridine derivatives （II） have been designed and synthesized. The target com- pounds have been identified by elemental analysis and spectral （1H NMR, IR, and MS） data and the absolute configuration of compound （IIl） was confirmed by single crystal X-ray diffraction. The cytotoxicity of the target compounds have been evalu- ated in vitro against two human breast cancer cell lines MCF-7 and MDA-MB-231 by MTT assay. Most compounds exhibited good inhibition, and compounds II21 （IC50 = 4.7 μM for MCF-7 and IC50 = 9.3 μM for MDA-MB-231）, 1133 （IC50 = 2.4 μM for MCF-7 and IC50 = 4.2 gM for MDA-MB-231） and 114o （IC50 = 3.3 μM for MCF-7 and IC5o =8.6 μM for MDA-MB-231） dis- played better inhibitory activity than 5-fluorouracil （IC50 = 4.8 μM for MCF-7 and IC50 = 9.6 I, tM for MDA-MB-231, respec- tively）. Flow cytometric analysis and DNA fragmentation suggest that II33 is cytotoxic and able to induce the apoptosis of MCF-7 cells. The fluorescence properties of compounds IIl, II6, II11,II16, II23, Il2s, and II3s were also studied and compound Ilzs afforded the highest photoluminescence quantum yield （38%）.
