The genus Diaporthe(Phomopsis)includes important plant pathogenic fungi with wide host ranges and geographic distributions.In the present study,phylogenetic species recognition in Diaporthe is re-evaluated using a mul...The genus Diaporthe(Phomopsis)includes important plant pathogenic fungi with wide host ranges and geographic distributions.In the present study,phylogenetic species recognition in Diaporthe is re-evaluated using a multi-locus phylogeny based on a combined data matrix of rDNA ITS,and partial sequences from the translation elongation factor 1-α(EF 1-α),βtubulin(TUB)and calmodulin(CAL)molecular markers.DNA sequences of available extype cultures have been included,providing a multi-locus backbone tree for future studies on Diaporthe.Four utilizable loci were analyzed individually and in combination,and ITS,EF 1-αand multi-locus phylogenetic trees are presented.The phylogenetic tree inferred by combined analysis of four loci provided the best resolution for species as compared to single gene analysis.Notes are provided for nine species previously known in Phomopsis that are transferred to Diaporthe in the present study.The unraveling of cryptic species complexes of Diaporthe based on Genealogical Concordance Phylogenetic Species Recognition(GCPSR)is emphasized.展开更多
以黄栌的当年生嫩枝茎段为外植体进行簇生芽诱导,对黄栌的快繁技术进行研究。结果表明,诱导簇生芽时,在M S+6-BA 2.0 m g/L+NAA 0.2 m g/L培养基上的诱导效果较好,分化率达100%;继代培养时,以M S+6-BA 1.0 m g/L+KT 0.5 m g/L+NAA 0.1 m...以黄栌的当年生嫩枝茎段为外植体进行簇生芽诱导,对黄栌的快繁技术进行研究。结果表明,诱导簇生芽时,在M S+6-BA 2.0 m g/L+NAA 0.2 m g/L培养基上的诱导效果较好,分化率达100%;继代培养时,以M S+6-BA 1.0 m g/L+KT 0.5 m g/L+NAA 0.1 m g/L培养基的增殖倍数最高,达11.8倍;再生苗在1/2 M S+NAA 0.1 m g/L培养基上生长良好;在炼苗中,移栽基质以蛭石+森林土较好,其有助于小苗的后期生长。展开更多
基金supported by the State Key Laboratory of Mycology,Institute of Microbiology by grant NFSC Y2JJ011002Thailand Research fund BRG 52800002.
文摘The genus Diaporthe(Phomopsis)includes important plant pathogenic fungi with wide host ranges and geographic distributions.In the present study,phylogenetic species recognition in Diaporthe is re-evaluated using a multi-locus phylogeny based on a combined data matrix of rDNA ITS,and partial sequences from the translation elongation factor 1-α(EF 1-α),βtubulin(TUB)and calmodulin(CAL)molecular markers.DNA sequences of available extype cultures have been included,providing a multi-locus backbone tree for future studies on Diaporthe.Four utilizable loci were analyzed individually and in combination,and ITS,EF 1-αand multi-locus phylogenetic trees are presented.The phylogenetic tree inferred by combined analysis of four loci provided the best resolution for species as compared to single gene analysis.Notes are provided for nine species previously known in Phomopsis that are transferred to Diaporthe in the present study.The unraveling of cryptic species complexes of Diaporthe based on Genealogical Concordance Phylogenetic Species Recognition(GCPSR)is emphasized.
文摘以黄栌的当年生嫩枝茎段为外植体进行簇生芽诱导,对黄栌的快繁技术进行研究。结果表明,诱导簇生芽时,在M S+6-BA 2.0 m g/L+NAA 0.2 m g/L培养基上的诱导效果较好,分化率达100%;继代培养时,以M S+6-BA 1.0 m g/L+KT 0.5 m g/L+NAA 0.1 m g/L培养基的增殖倍数最高,达11.8倍;再生苗在1/2 M S+NAA 0.1 m g/L培养基上生长良好;在炼苗中,移栽基质以蛭石+森林土较好,其有助于小苗的后期生长。
