Objective:To develop a clinically applicable tool for predicting clinical pregnancy,providing individualized patient counseling,and helping couples with non-obstructive azoospermia(NOA)decide whether to use fresh or c...Objective:To develop a clinically applicable tool for predicting clinical pregnancy,providing individualized patient counseling,and helping couples with non-obstructive azoospermia(NOA)decide whether to use fresh or cryopreserved spermatozoa for oocyte insemination before microdissection testicular sperm extraction(mTESE).Methods:A total of 240 couples with NOA who underwent mTESE-ICSI were divided into two groups based on the type of spermatozoa used for intracytoplasmic sperm injection(ICSI):the fresh and cryopreserved groups.After evaluating several machine learning algorithms,logistic regression was selected.Using LASSO regression and 10-fold cross-validation,the factors associated with clinical pregnancy were analyzed.Results:The area under the curves(AUCs)for the fresh and cryopreserved groups in the Logistic Regression-based prediction model were 0.977 and 0.759,respectively.Compared with various modeling algorithms,Logistic Regression outperformed machine learning in both groups,with an AUC of 0.945 for the fresh group and 0.788 for the cryopreserved group.Conclusion:The model accurately predicted clinical pregnancies in NOA couples.展开更多
Objective: Comparison of vaginal progesterone (VP) versus VP and intermittent intramuscular progesterone (IMP) use in frozen/thawed blastocyst transfer cycles. Study Design: A single center retrospective analyses of 4...Objective: Comparison of vaginal progesterone (VP) versus VP and intermittent intramuscular progesterone (IMP) use in frozen/thawed blastocyst transfer cycles. Study Design: A single center retrospective analyses of 470 elective FET cycles which were performed between January 2015 and September 2019 were evaluated. Patients were divided into two groups. Control group was consisted of VP (n = 272), the study group was consisted of VP plus IMP (n = 198) users. Results: The number of transfer attempts in control and study groups was 272 and 198, respectively. Age (29.8 ± 4 vs 30.6 ± 4;p = 0.09), BMI (22 ± 2 vs 21.9 ± 3;p = 0.79) and the number of transferred embryos (1.4 ± 0.5 vs 1.4 ± 0.5;p = 0.48) were comparable between groups. Altough, implantation rates (43.7% vs 43.6%;p = 0.9), ectopic pregnancy (0.8% vs 0.3%;p = 0.46) and abortion rates (8.2% vs 4.8%;p = 0.07) were similar. Biochemical pregnancy rate (8.4% vs 3.4% p = 0.01) in control group and ongoing pregnancy rate (OPR) (27.9% vs 38.1%;p = 0.005) in study group were significantly higher. Conclusion: Within the FET cycles in which good quality blastocyst are being transferred additional IMP supplementation to VP may increase OPR while reducing the biochemical pregnancy rate.展开更多
Objective: This study reports the outcomes of the cryopreserved mitral homograft in 119 patients prospectively followed with clinical, echocardiographic and structural valve deterioration assessments. Methods: 119 pat...Objective: This study reports the outcomes of the cryopreserved mitral homograft in 119 patients prospectively followed with clinical, echocardiographic and structural valve deterioration assessments. Methods: 119 patients undergoing mitral and aortic homograft implantation. Patient’s causes of mitral disease were: rheumatic disease (n = 75), endocarditis (n = 37) and others (n = 7). There were 40 partial homografts and 88 total homografts. Mitro-aortic homograft valve replacement was performed in 29 cases. Results: Mean follow-up was 9.8 ± 6.3 years (up to 19.2 yrs). There were 7 early (2展开更多
Post-operative adhesions,a common complication of surgery,cause pain,impair organ functionality,and often require additional surgical interventions.Control of inflammation,protection of injured tissue,and rapid tissue...Post-operative adhesions,a common complication of surgery,cause pain,impair organ functionality,and often require additional surgical interventions.Control of inflammation,protection of injured tissue,and rapid tissue repair are critical for adhesion prevention.Adhesion barriers are biomaterials used to prevent adhesions by physical separation of opposing injured tissues.Current adhesion barriers have poor anti-inflammatory and tissue regenerative properties.Umbilical cord tissue(UT),a part of the placenta,is inherently soft,conforming,biocompatible,and biodegradable,with antimicrobial,anti-inflammatory,and antifibrotic properties,making it an attractive alternative to currently available adhesion barriers.While use of fresh tissue is preferable,availability and short storage time limit its clinical use.A viable cryopreserved UT(vCUT)“point of care”allograft has recently become available.vCUT retains the extracellular matrix,growth factors,and native viable cells with the added advantage of a long shelf life at−80℃.In this study,vCUT's anti-adhesion property was evaluated in a rabbit abdominal adhesion model.The cecum was abraded on two opposing sides,and vCUT was sutured to the abdominal wall on the treatment side;whereas the contralateral side of the abdomen served as an internal untreated control.Gross and histological evaluation was performed at 7,28,and 67 days post-surgery.No adhesions were detectable on the vCUT treated side at all time points.Histological scores for adhesion,inflammation,and fibrosis were lower on the vCUT treated side as compared to the control side.In conclusion,the data supports the use of vCUT as an adhesion barrier in surgical procedures.展开更多
To Editor,We read with interest the article entitled ＂Transplantation of cryopreserved teeth： a systematic review＂ （Osathanon, 2010）. Although the author is to be congratulated for his systematic approach to cryo...To Editor,We read with interest the article entitled ＂Transplantation of cryopreserved teeth： a systematic review＂ （Osathanon, 2010）. Although the author is to be congratulated for his systematic approach to cryopreserved tooth transplantation （CTT）, we would like to draw your attention to some technical limitations of this review.展开更多
Traditional radiotherapy and chemotherapy often cause irreversible damage to the fertility and endocrine function of cancer patients.The current methods of fertility preservation include freezing the sperms of adult a...Traditional radiotherapy and chemotherapy often cause irreversible damage to the fertility and endocrine function of cancer patients.The current methods of fertility preservation include freezing the sperms of adult and adolescent males after puberty;freezing the embryos,oocytes,and ovarian tissue of females;and drug intervention and fertility preservation surgery.This article reviews fertility preservation in cancer patients with respect to current methods,indications,and some more recently developed methods that remain under investigation.展开更多
基金National Natural Science Foundation of China(82271651)。
文摘Objective:To develop a clinically applicable tool for predicting clinical pregnancy,providing individualized patient counseling,and helping couples with non-obstructive azoospermia(NOA)decide whether to use fresh or cryopreserved spermatozoa for oocyte insemination before microdissection testicular sperm extraction(mTESE).Methods:A total of 240 couples with NOA who underwent mTESE-ICSI were divided into two groups based on the type of spermatozoa used for intracytoplasmic sperm injection(ICSI):the fresh and cryopreserved groups.After evaluating several machine learning algorithms,logistic regression was selected.Using LASSO regression and 10-fold cross-validation,the factors associated with clinical pregnancy were analyzed.Results:The area under the curves(AUCs)for the fresh and cryopreserved groups in the Logistic Regression-based prediction model were 0.977 and 0.759,respectively.Compared with various modeling algorithms,Logistic Regression outperformed machine learning in both groups,with an AUC of 0.945 for the fresh group and 0.788 for the cryopreserved group.Conclusion:The model accurately predicted clinical pregnancies in NOA couples.
文摘Objective: Comparison of vaginal progesterone (VP) versus VP and intermittent intramuscular progesterone (IMP) use in frozen/thawed blastocyst transfer cycles. Study Design: A single center retrospective analyses of 470 elective FET cycles which were performed between January 2015 and September 2019 were evaluated. Patients were divided into two groups. Control group was consisted of VP (n = 272), the study group was consisted of VP plus IMP (n = 198) users. Results: The number of transfer attempts in control and study groups was 272 and 198, respectively. Age (29.8 ± 4 vs 30.6 ± 4;p = 0.09), BMI (22 ± 2 vs 21.9 ± 3;p = 0.79) and the number of transferred embryos (1.4 ± 0.5 vs 1.4 ± 0.5;p = 0.48) were comparable between groups. Altough, implantation rates (43.7% vs 43.6%;p = 0.9), ectopic pregnancy (0.8% vs 0.3%;p = 0.46) and abortion rates (8.2% vs 4.8%;p = 0.07) were similar. Biochemical pregnancy rate (8.4% vs 3.4% p = 0.01) in control group and ongoing pregnancy rate (OPR) (27.9% vs 38.1%;p = 0.005) in study group were significantly higher. Conclusion: Within the FET cycles in which good quality blastocyst are being transferred additional IMP supplementation to VP may increase OPR while reducing the biochemical pregnancy rate.
文摘Objective: This study reports the outcomes of the cryopreserved mitral homograft in 119 patients prospectively followed with clinical, echocardiographic and structural valve deterioration assessments. Methods: 119 patients undergoing mitral and aortic homograft implantation. Patient’s causes of mitral disease were: rheumatic disease (n = 75), endocarditis (n = 37) and others (n = 7). There were 40 partial homografts and 88 total homografts. Mitro-aortic homograft valve replacement was performed in 29 cases. Results: Mean follow-up was 9.8 ± 6.3 years (up to 19.2 yrs). There were 7 early (2
文摘Post-operative adhesions,a common complication of surgery,cause pain,impair organ functionality,and often require additional surgical interventions.Control of inflammation,protection of injured tissue,and rapid tissue repair are critical for adhesion prevention.Adhesion barriers are biomaterials used to prevent adhesions by physical separation of opposing injured tissues.Current adhesion barriers have poor anti-inflammatory and tissue regenerative properties.Umbilical cord tissue(UT),a part of the placenta,is inherently soft,conforming,biocompatible,and biodegradable,with antimicrobial,anti-inflammatory,and antifibrotic properties,making it an attractive alternative to currently available adhesion barriers.While use of fresh tissue is preferable,availability and short storage time limit its clinical use.A viable cryopreserved UT(vCUT)“point of care”allograft has recently become available.vCUT retains the extracellular matrix,growth factors,and native viable cells with the added advantage of a long shelf life at−80℃.In this study,vCUT's anti-adhesion property was evaluated in a rabbit abdominal adhesion model.The cecum was abraded on two opposing sides,and vCUT was sutured to the abdominal wall on the treatment side;whereas the contralateral side of the abdomen served as an internal untreated control.Gross and histological evaluation was performed at 7,28,and 67 days post-surgery.No adhesions were detectable on the vCUT treated side at all time points.Histological scores for adhesion,inflammation,and fibrosis were lower on the vCUT treated side as compared to the control side.In conclusion,the data supports the use of vCUT as an adhesion barrier in surgical procedures.
文摘To Editor,We read with interest the article entitled ＂Transplantation of cryopreserved teeth： a systematic review＂ （Osathanon, 2010）. Although the author is to be congratulated for his systematic approach to cryopreserved tooth transplantation （CTT）, we would like to draw your attention to some technical limitations of this review.
基金Gansu Province Science Foundation for Distinguished Young Scholars(Grant No.18JR3RA262)。
文摘Traditional radiotherapy and chemotherapy often cause irreversible damage to the fertility and endocrine function of cancer patients.The current methods of fertility preservation include freezing the sperms of adult and adolescent males after puberty;freezing the embryos,oocytes,and ovarian tissue of females;and drug intervention and fertility preservation surgery.This article reviews fertility preservation in cancer patients with respect to current methods,indications,and some more recently developed methods that remain under investigation.
