The recycling of biowaste from municipal wastewater treatment plants(WWTPs)in agriculture represents a circular economy-driven source of water and nutrients to support food system sustainability.However,biowaste may r...The recycling of biowaste from municipal wastewater treatment plants(WWTPs)in agriculture represents a circular economy-driven source of water and nutrients to support food system sustainability.However,biowaste may represent the source of emerging hazards of anthropogenic and animal origin that can transfer from agricultural soils to related food production,posing a risk to consumers’health,as in the case of outbreaks due to the consumption of ready-to-eat leafy vegetables contaminated with pathogenic E.coli.From this perspective,we propose a combined strategy based on both classical methods and culture-independent metagenomics approaches to identify microbial hazards relevant to foodborne diseases in WWTP-related biowastes.The virulence genes targeted by real-time PCR,performed before and after the enrichment of the raw samples,may represent a proxy for the viability of pathogens,the presence of which is then confirmed via classical microbiological methods.Bioinformatics analysis of shotgun metagenomic sequences could assess the presence of genes associated with resistance to specific antimicrobials followed by phenotypic confirmation via cultivation of the raw samples in the presence of the predicted molecules.Bacterial 16S rDNA analysis supports biowaste traceability based on their taxonomic composition.This strategy would support a“One Health”Action based on a cross-cutting assessment of emerging food-borne risks along the food chain.展开更多
罗拉·布瑞格斯(Laura Briggs)教授是美国马萨诸塞大学阿默斯特分校(University of Massachusetts Amherst)性别研究专业(Women, Gender, and Sexuality Studies Department)系主任。该校性别研究专业创建于20世纪70年代,与马萨诸...罗拉·布瑞格斯(Laura Briggs)教授是美国马萨诸塞大学阿默斯特分校(University of Massachusetts Amherst)性别研究专业(Women, Gender, and Sexuality Studies Department)系主任。该校性别研究专业创建于20世纪70年代,与马萨诸塞州先锋谷的史密斯女子学院(Smith College)和曼荷莲女子学院(Mount Holyoke College)等五所高校组成的妇女研究中心(Five College Women’s Studies Research Center)一起构成北美性别研究领域的重镇。展开更多
Group dynamics is applied not only in the professional life but also in the educational framework becoming one of the tools of group therapy aiming at reducing conflicts and tensions.Under this perspective it would be...Group dynamics is applied not only in the professional life but also in the educational framework becoming one of the tools of group therapy aiming at reducing conflicts and tensions.Under this perspective it would be interesting to study the dynamic exchanges that take place within the interrelations of the facilitator with his target group aiming at further exploiting the cognitive and affective resources of the participants in order to manage their potential more effectively and to accompany them in any training situation.From an actional perspective the adoption of collaborative tasks promotes cooperation and problem solving in a mentoring context,where the members invest in the realization of a meaningful action project in order to implement creative tasks and cultivate transversal skills using digital technology.展开更多
35 k V线路的投运时间较长,原线路的设计标准较低。部分35 k V线路的对地距离、对交跨距离严重不足,加之受地形影响,部分线路无法加高,导致夏季高温弧垂增大,严重影响了线路的安全、稳定运行。因此,研究、设计了35 k V线路ZS型单杆加高...35 k V线路的投运时间较长,原线路的设计标准较低。部分35 k V线路的对地距离、对交跨距离严重不足,加之受地形影响,部分线路无法加高,导致夏季高温弧垂增大,严重影响了线路的安全、稳定运行。因此,研究、设计了35 k V线路ZS型单杆加高顶架,以提高对地和交叉跨越的安全,提高架空输电线路的安全性和可靠性,从而达到缩短线路停电时间、降低线路改造成本的目的。展开更多
基金supported by the Italian Ministry of Health grant no.RF-2019-12369714,PI Stefano Morabito.
文摘The recycling of biowaste from municipal wastewater treatment plants(WWTPs)in agriculture represents a circular economy-driven source of water and nutrients to support food system sustainability.However,biowaste may represent the source of emerging hazards of anthropogenic and animal origin that can transfer from agricultural soils to related food production,posing a risk to consumers’health,as in the case of outbreaks due to the consumption of ready-to-eat leafy vegetables contaminated with pathogenic E.coli.From this perspective,we propose a combined strategy based on both classical methods and culture-independent metagenomics approaches to identify microbial hazards relevant to foodborne diseases in WWTP-related biowastes.The virulence genes targeted by real-time PCR,performed before and after the enrichment of the raw samples,may represent a proxy for the viability of pathogens,the presence of which is then confirmed via classical microbiological methods.Bioinformatics analysis of shotgun metagenomic sequences could assess the presence of genes associated with resistance to specific antimicrobials followed by phenotypic confirmation via cultivation of the raw samples in the presence of the predicted molecules.Bacterial 16S rDNA analysis supports biowaste traceability based on their taxonomic composition.This strategy would support a“One Health”Action based on a cross-cutting assessment of emerging food-borne risks along the food chain.
文摘罗拉·布瑞格斯(Laura Briggs)教授是美国马萨诸塞大学阿默斯特分校(University of Massachusetts Amherst)性别研究专业(Women, Gender, and Sexuality Studies Department)系主任。该校性别研究专业创建于20世纪70年代,与马萨诸塞州先锋谷的史密斯女子学院(Smith College)和曼荷莲女子学院(Mount Holyoke College)等五所高校组成的妇女研究中心(Five College Women’s Studies Research Center)一起构成北美性别研究领域的重镇。
文摘Group dynamics is applied not only in the professional life but also in the educational framework becoming one of the tools of group therapy aiming at reducing conflicts and tensions.Under this perspective it would be interesting to study the dynamic exchanges that take place within the interrelations of the facilitator with his target group aiming at further exploiting the cognitive and affective resources of the participants in order to manage their potential more effectively and to accompany them in any training situation.From an actional perspective the adoption of collaborative tasks promotes cooperation and problem solving in a mentoring context,where the members invest in the realization of a meaningful action project in order to implement creative tasks and cultivate transversal skills using digital technology.
文摘35 k V线路的投运时间较长,原线路的设计标准较低。部分35 k V线路的对地距离、对交跨距离严重不足,加之受地形影响,部分线路无法加高,导致夏季高温弧垂增大,严重影响了线路的安全、稳定运行。因此,研究、设计了35 k V线路ZS型单杆加高顶架,以提高对地和交叉跨越的安全,提高架空输电线路的安全性和可靠性,从而达到缩短线路停电时间、降低线路改造成本的目的。
