Acetylcholinesterase (AChE) is the target of organophosphate and carbamate pesticides. Organophosphate resistance is worldspread in the cotton bollworm[Helicoverpa armigera (Hübner)]. With the degenerate primers ...Acetylcholinesterase (AChE) is the target of organophosphate and carbamate pesticides. Organophosphate resistance is worldspread in the cotton bollworm[Helicoverpa armigera (Hübner)]. With the degenerate primers we amplified a 281 bp cDNA fragment of acetylcholinesterase (AChE) gene in H. armigera by reverse transcription-polymerase chain reaction (RT-PCR) method using total RNA extracted from 4th larva as the template. The cDNA fragment was inserted into pGEMT vector and then cloned. The deduced amino acid sequence of AChE consisted of 94 residues. The sequence analysis indicated that the deduced amino acid sequence of the cDNA fragment shares high identity with AChE gene from other published insects and animals. The acquired sequence had 84%,79%,74%,70%,70%,72%,68%,61%,55% and 57% of amino acid residues identical to those of Leptinotarsa decemlineata(L.d.), Nephotettix cincticeps(N.c.),Anopheles stephensi(A.s.), Aedes aegypti(A.a.), Lucilia cuprina(L.c.), Drosophila melanogaster(D.m.), Musca domestica(M.d.), Meloidogyne incognita(M.i.), Torpedo californica(T.c.) and Gallus gallus(G.g.), respectively. All these results firmly established that the amplified cDNA fragment was the partial sequence of AChE gene in H. armigera. This is the first report of partial cDNA sequence of AChE in H. armigera.展开更多
运用地统计学方法，分析了山东省滨州地区二代棉铃虫在1965-2004年间种群动态的时间序列结构特征和自相关性，根据决定系数尺。和Akaike’s Information Criteria（AIC）判别参数，筛选出最优拟合模型。结果表明：棉铃虫越冬蛹量、二代...运用地统计学方法，分析了山东省滨州地区二代棉铃虫在1965-2004年间种群动态的时间序列结构特征和自相关性，根据决定系数尺。和Akaike’s Information Criteria（AIC）判别参数，筛选出最优拟合模型。结果表明：棉铃虫越冬蛹量、二代棉铃虫百株累计卵量、发生程度的最优拟合模型均是球形模型，呈聚集分布；三者的变程分别为10．41、12和12．06年，高度一致；三者在时间序列上的自相关性分别是53．22％、52．62％和69．06％；三者的结构特征引起的种群变异介于25％～75％，为中等程度的空间自相关性，其相关强度顺序是百株累计卵量〈越冬蛹量〈发生程度，随机部分引起的空间变异则相反。展开更多
文摘Acetylcholinesterase (AChE) is the target of organophosphate and carbamate pesticides. Organophosphate resistance is worldspread in the cotton bollworm[Helicoverpa armigera (Hübner)]. With the degenerate primers we amplified a 281 bp cDNA fragment of acetylcholinesterase (AChE) gene in H. armigera by reverse transcription-polymerase chain reaction (RT-PCR) method using total RNA extracted from 4th larva as the template. The cDNA fragment was inserted into pGEMT vector and then cloned. The deduced amino acid sequence of AChE consisted of 94 residues. The sequence analysis indicated that the deduced amino acid sequence of the cDNA fragment shares high identity with AChE gene from other published insects and animals. The acquired sequence had 84%,79%,74%,70%,70%,72%,68%,61%,55% and 57% of amino acid residues identical to those of Leptinotarsa decemlineata(L.d.), Nephotettix cincticeps(N.c.),Anopheles stephensi(A.s.), Aedes aegypti(A.a.), Lucilia cuprina(L.c.), Drosophila melanogaster(D.m.), Musca domestica(M.d.), Meloidogyne incognita(M.i.), Torpedo californica(T.c.) and Gallus gallus(G.g.), respectively. All these results firmly established that the amplified cDNA fragment was the partial sequence of AChE gene in H. armigera. This is the first report of partial cDNA sequence of AChE in H. armigera.
文摘运用地统计学方法，分析了山东省滨州地区二代棉铃虫在1965-2004年间种群动态的时间序列结构特征和自相关性，根据决定系数尺。和Akaike’s Information Criteria（AIC）判别参数，筛选出最优拟合模型。结果表明：棉铃虫越冬蛹量、二代棉铃虫百株累计卵量、发生程度的最优拟合模型均是球形模型，呈聚集分布；三者的变程分别为10．41、12和12．06年，高度一致；三者在时间序列上的自相关性分别是53．22％、52．62％和69．06％；三者的结构特征引起的种群变异介于25％～75％，为中等程度的空间自相关性，其相关强度顺序是百株累计卵量〈越冬蛹量〈发生程度，随机部分引起的空间变异则相反。
