Chemical investigation of the organic solvent extract of Cretaceous Ginkgo coriacea Florin leaves by liquid chromatography-mass spectroscopy （LC-MS） and gas chromatography-mass spectrometry （GC-MS）, analogous ...Chemical investigation of the organic solvent extract of Cretaceous Ginkgo coriacea Florin leaves by liquid chromatography-mass spectroscopy （LC-MS） and gas chromatography-mass spectrometry （GC-MS）, analogous to those from extant leaves of Ginkgo biloba L., led to the detection of a group of natural flavonoids and other volatiles. The similarity of the chemical constituents in these two species of Ginkgo suggest that the secondary metabolism of extant G. biloba is close to that of the Cretaceous species. The remaining natural products may be one explanation why the leaves of the Cretaceous G. coriacea have been preserved morphologically in fossilization. The detection of flavonoids suggests that the leaves of G. coriacea experienced a mild post-depositional environment during their fossilization. This appears to be the oldest occurrence of flavonoids in plant fossils.展开更多
The present study was carried out with the objective of evaluating, in castrated rats, the utero trophic, hormonal and biochemical activities of aqueous extracts of Buchholzia coriacea (BC) and Cogniauxia podolaena (C...The present study was carried out with the objective of evaluating, in castrated rats, the utero trophic, hormonal and biochemical activities of aqueous extracts of Buchholzia coriacea (BC) and Cogniauxia podolaena (CP) leaves. Each extract administered at the dose of 600 mg/Kg in castrated rats did not cause a significant change in the fresh weight/dry weight ratio of the uterus compared to castrated rats given distilled water. However, those receiving 17-β-estradiol as a reference product showed a significant (p < 0.5) increase in this ratio. These results indicate the absence of uterotrophic effects of both extracts in the ovariectomized rat compared with the effects of 17-β estradiol. In addition, the extracts did not cause significant changes in estrogen or progesterone levels in treated rats, as observed with 17-β-estradiol. In addition, the determination of protein and total cholesterol in the uterus of castrated rats treated with each extract did not show significant variation from controls. At the time, castrated rats treated with 17-β-estradiol showed a significant increase (p < 0.5) in uterine protein level and a significant decrease (p < 0.5) in total cholesterol level. Only the blood protein level was significantly increased in the castrated rats that received the extracts. These results suggest that the respective estrogenic and progesterone effects of the extracts of the two plants may be ovarian-dependent, these plants would not contain phytohormones.展开更多
Background: Different studies have unveiled the traditional usefulness and clinical potentials of Buchholzia coriacea, a medicinal plant known for its effectiveness in lowering blood glucose. Its role in intestinal gl...Background: Different studies have unveiled the traditional usefulness and clinical potentials of Buchholzia coriacea, a medicinal plant known for its effectiveness in lowering blood glucose. Its role in intestinal glucose uptake was investigated. Materials and methods: Thirty male Wistar rats, weighing between 100 - 120 g were used and randomly assigned into three groups of 5 rats each per experiment. Group 1: control (not treated), groups 2 and 3, were treated with 100 mg/kg (BC100) and 200 mg/kg (BC200) of Buchholzia coriacea orally respectively for 2 weeks. Fasting blood glucose, luminal and in vitro glucose levels of rats were determined by glucose oxidase method using glucometer stripes plus glucose monitoring system (Fine test glucometer(R) ). Luminal electrolytes in the in vitro study were determined by Atomic Absorption Spectrophotometry method. Data were expressed as Mean ± SEM and statistical analysis was by one way ANOVA, and p-values Results: There was no significant change in the fasting blood glucose level (mg/dl) of rats in BC100 (78.00 ± 2.16) and BC200 (76.0 ± 3.57) compared with control (79.50 ± 1.70). There was significant increase in glucose uptake (mg/dl/g tissue) in the in vivo experiments in both the ileum and jejunum of BC100 (23.08 ± 0.18;19.68 ± 0.72) and BC200 (14.50 ± 1.02;20.55 ± 0.45) compared with control (30.40 ± 1.01;35.53 ± 1.45), respectively. The glucose uptake at the mucosa end of distal jejunum in the BC100 (292 ± 3.33) and BC200 (209.30 ± 2.67) decreased significantly compared with control (90.00 ± 1.50). There was a significant decrease in K+ concentration and increase in Na+ concentration at the mucosa end in the BC100 and BC200 compared with control. Conclusion: The study suggests glucose stowing in the intestinal cells in the in vivo study and inhibition of glucose transport from the in vitro study. The roles of alpha-amylase on the activity of this extract are suggested for future studies.展开更多
Objective: To investigate the anti-colitic effects of diet containing seeds of Buchholzia coriacea(B. coriacea) on acetic acid induced ulcerative colitis. Methods: Male Wistar rats(70-100 g) were fed with standard die...Objective: To investigate the anti-colitic effects of diet containing seeds of Buchholzia coriacea(B. coriacea) on acetic acid induced ulcerative colitis. Methods: Male Wistar rats(70-100 g) were fed with standard diets(control group) or with same diet containing B. coriacea seeds at 12.5% or 25% for six weeks. At the sixth week, colitis was induced by intra-rectal administration of 1 mL/200 g 6% acetic acid. Animals were sacrificed at days 0(before induction), 1, 3 and 7 post induction to assess disease severity via evaluation of stool consistency, haematological variables and histomorphometry of colon tissues. Results: A significant increase in body weight was observed in the 12.5% B. coriacea fed group compared to the control. B. coriacea significantly reduced stool consistency and microscopic scores. Histological evaluation revealed significantly decreased inflammatory aggregates in B. coriacea fed groups compared to control after colitis induction. There was a significant decrease in packed cell volume, red blood cell and haemoglobin concentration in the control group at day 1 post colitis induction compared to 12.5% B. coriacea fed groups. Neutrophils and white blood cell were also significantly increased after colitis induction in the control group while these were significantly decreased in the B. coriacea fed groups. Conclusions: The addition of B. coriacea seeds to diets promotes healing of acetic acid induced colitis by suppressing infiltration of inflammatory aggregates and ameliorating anemia.展开更多
Investigation was carried out on the significance of seed size on morphological traits in seedlings of Buchholzia eoriaeea Engler, a medicinal plant in Southwestern Nigeria. Three size classes-large （seeds 〉 40 g）,...Investigation was carried out on the significance of seed size on morphological traits in seedlings of Buchholzia eoriaeea Engler, a medicinal plant in Southwestern Nigeria. Three size classes-large （seeds 〉 40 g）, medium （seeds between 25 and 40 g） and small （seed 〈 25 g） were used. Ninety uniformly growing seedlings from each size class were assessed for shoot height, number of leaves, collar diameter, leaf area and biomass production for six months. Seed size showed significant effect on height growth, collar diameter and number of leaves （P 〈 0.05）. Seedlings from large seed size class had the best height and collar diameter （45.3 cm, 8.7 mm）. Seeds of large and medium seed size classes had overall means of 15.6 and 15.5 leaves/seedling respectively. Leaf dry weight, stem dry weight and root biomass in seedlings from the three seed size classes were significantly different at P 〈 0.05 with the highest mean values for leaf dry weight, stem dry weight and root biomass recorded by seedlings from the large seed size class. The results showed that seed size has great influence on seedling growth when they are young. With availability of sufficient nutrient and light, effect of seed size on seedling growth and development could disappear as seedlings attain maturity.展开更多
基金Supported by the National Natural Science Foundation of China(40403014)the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX1-10-01 and KZCX2-SW-118)
文摘Chemical investigation of the organic solvent extract of Cretaceous Ginkgo coriacea Florin leaves by liquid chromatography-mass spectroscopy （LC-MS） and gas chromatography-mass spectrometry （GC-MS）, analogous to those from extant leaves of Ginkgo biloba L., led to the detection of a group of natural flavonoids and other volatiles. The similarity of the chemical constituents in these two species of Ginkgo suggest that the secondary metabolism of extant G. biloba is close to that of the Cretaceous species. The remaining natural products may be one explanation why the leaves of the Cretaceous G. coriacea have been preserved morphologically in fossilization. The detection of flavonoids suggests that the leaves of G. coriacea experienced a mild post-depositional environment during their fossilization. This appears to be the oldest occurrence of flavonoids in plant fossils.
文摘The present study was carried out with the objective of evaluating, in castrated rats, the utero trophic, hormonal and biochemical activities of aqueous extracts of Buchholzia coriacea (BC) and Cogniauxia podolaena (CP) leaves. Each extract administered at the dose of 600 mg/Kg in castrated rats did not cause a significant change in the fresh weight/dry weight ratio of the uterus compared to castrated rats given distilled water. However, those receiving 17-β-estradiol as a reference product showed a significant (p < 0.5) increase in this ratio. These results indicate the absence of uterotrophic effects of both extracts in the ovariectomized rat compared with the effects of 17-β estradiol. In addition, the extracts did not cause significant changes in estrogen or progesterone levels in treated rats, as observed with 17-β-estradiol. In addition, the determination of protein and total cholesterol in the uterus of castrated rats treated with each extract did not show significant variation from controls. At the time, castrated rats treated with 17-β-estradiol showed a significant increase (p < 0.5) in uterine protein level and a significant decrease (p < 0.5) in total cholesterol level. Only the blood protein level was significantly increased in the castrated rats that received the extracts. These results suggest that the respective estrogenic and progesterone effects of the extracts of the two plants may be ovarian-dependent, these plants would not contain phytohormones.
文摘Background: Different studies have unveiled the traditional usefulness and clinical potentials of Buchholzia coriacea, a medicinal plant known for its effectiveness in lowering blood glucose. Its role in intestinal glucose uptake was investigated. Materials and methods: Thirty male Wistar rats, weighing between 100 - 120 g were used and randomly assigned into three groups of 5 rats each per experiment. Group 1: control (not treated), groups 2 and 3, were treated with 100 mg/kg (BC100) and 200 mg/kg (BC200) of Buchholzia coriacea orally respectively for 2 weeks. Fasting blood glucose, luminal and in vitro glucose levels of rats were determined by glucose oxidase method using glucometer stripes plus glucose monitoring system (Fine test glucometer(R) ). Luminal electrolytes in the in vitro study were determined by Atomic Absorption Spectrophotometry method. Data were expressed as Mean ± SEM and statistical analysis was by one way ANOVA, and p-values Results: There was no significant change in the fasting blood glucose level (mg/dl) of rats in BC100 (78.00 ± 2.16) and BC200 (76.0 ± 3.57) compared with control (79.50 ± 1.70). There was significant increase in glucose uptake (mg/dl/g tissue) in the in vivo experiments in both the ileum and jejunum of BC100 (23.08 ± 0.18;19.68 ± 0.72) and BC200 (14.50 ± 1.02;20.55 ± 0.45) compared with control (30.40 ± 1.01;35.53 ± 1.45), respectively. The glucose uptake at the mucosa end of distal jejunum in the BC100 (292 ± 3.33) and BC200 (209.30 ± 2.67) decreased significantly compared with control (90.00 ± 1.50). There was a significant decrease in K+ concentration and increase in Na+ concentration at the mucosa end in the BC100 and BC200 compared with control. Conclusion: The study suggests glucose stowing in the intestinal cells in the in vivo study and inhibition of glucose transport from the in vitro study. The roles of alpha-amylase on the activity of this extract are suggested for future studies.
文摘Objective: To investigate the anti-colitic effects of diet containing seeds of Buchholzia coriacea(B. coriacea) on acetic acid induced ulcerative colitis. Methods: Male Wistar rats(70-100 g) were fed with standard diets(control group) or with same diet containing B. coriacea seeds at 12.5% or 25% for six weeks. At the sixth week, colitis was induced by intra-rectal administration of 1 mL/200 g 6% acetic acid. Animals were sacrificed at days 0(before induction), 1, 3 and 7 post induction to assess disease severity via evaluation of stool consistency, haematological variables and histomorphometry of colon tissues. Results: A significant increase in body weight was observed in the 12.5% B. coriacea fed group compared to the control. B. coriacea significantly reduced stool consistency and microscopic scores. Histological evaluation revealed significantly decreased inflammatory aggregates in B. coriacea fed groups compared to control after colitis induction. There was a significant decrease in packed cell volume, red blood cell and haemoglobin concentration in the control group at day 1 post colitis induction compared to 12.5% B. coriacea fed groups. Neutrophils and white blood cell were also significantly increased after colitis induction in the control group while these were significantly decreased in the B. coriacea fed groups. Conclusions: The addition of B. coriacea seeds to diets promotes healing of acetic acid induced colitis by suppressing infiltration of inflammatory aggregates and ameliorating anemia.
文摘Investigation was carried out on the significance of seed size on morphological traits in seedlings of Buchholzia eoriaeea Engler, a medicinal plant in Southwestern Nigeria. Three size classes-large （seeds 〉 40 g）, medium （seeds between 25 and 40 g） and small （seed 〈 25 g） were used. Ninety uniformly growing seedlings from each size class were assessed for shoot height, number of leaves, collar diameter, leaf area and biomass production for six months. Seed size showed significant effect on height growth, collar diameter and number of leaves （P 〈 0.05）. Seedlings from large seed size class had the best height and collar diameter （45.3 cm, 8.7 mm）. Seeds of large and medium seed size classes had overall means of 15.6 and 15.5 leaves/seedling respectively. Leaf dry weight, stem dry weight and root biomass in seedlings from the three seed size classes were significantly different at P 〈 0.05 with the highest mean values for leaf dry weight, stem dry weight and root biomass recorded by seedlings from the large seed size class. The results showed that seed size has great influence on seedling growth when they are young. With availability of sufficient nutrient and light, effect of seed size on seedling growth and development could disappear as seedlings attain maturity.
