A novel amperometric immunosensor based on the micro electromechanical systems （MEMS） technology, using protein A and self-assembled monolayers （SAMs） for the orientation-controlled immobilization of antibodies, h...A novel amperometric immunosensor based on the micro electromechanical systems （MEMS） technology, using protein A and self-assembled monolayers （SAMs） for the orientation-controlled immobilization of antibodies, has been developed. Using MEMS technology, an ＂Au, Pt, Pt＂ three-microelectrode system enclosed in a SU-8 micro pool was fabricated. Employing SAMs, a monolayer of protein A was immobilized on the cysteamine modified Au electrode to achieve the orientation-controlled immobilization of the human immunoglobulin （HIgG） antibody. The immunosensor aimed at low unit cost, small dimension, high level of integration and the prospect of a biosensor system-on-a-chip. Cyclic voltammetry and chronoamperometry were conducted to characterize the immunosensor. Compared with the traditional immunosensor using bulky gold electrode or screen-printed electrode and the procedure directly binding protein A to electrode for immobilization of antibodies, it had attractive advantages, such as miniaturization, compatibility with CMOS technology, fast response （30 s）, broad linear range （50-400 pg/L） and low detection limit （10 pg/L） for HIgG. In addition, this immunosensor was easy to be designed into micro array and to realize the simultaneously multi-parameter detection.展开更多
该文实现了一种基于电控旋转磁场的电磁跟踪系统。设计了以数字信号处理器(Digital Signal Processor,DSP)作为控制处理装置,并且包括一个可控恒流源模块、一个磁场源模块、一个三轴磁传感器及ADC接口电路的电磁跟踪系统。初步的实验测...该文实现了一种基于电控旋转磁场的电磁跟踪系统。设计了以数字信号处理器(Digital Signal Processor,DSP)作为控制处理装置,并且包括一个可控恒流源模块、一个磁场源模块、一个三轴磁传感器及ADC接口电路的电磁跟踪系统。初步的实验测试结果表明系统每次都能稳定实现定位,平均位置误差为0.282 cm,平均姿态误差为0.696°,定位时间为1.572 s。通过标定、校准以及硬件电路的进一步改进,该系统的性能有望进一步提高。展开更多
基金supported by the Open Fund of the State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation(Southwest Petroleum University,PLN2021-17)the Science and Technology Project of Southwest Petroleum University(2021JBGS08)Sichuan Science and Technology Program(2022YFSY0040)。
基金supported by the National Natural Science Foundation of China(Grant No.90307014).
文摘A novel amperometric immunosensor based on the micro electromechanical systems （MEMS） technology, using protein A and self-assembled monolayers （SAMs） for the orientation-controlled immobilization of antibodies, has been developed. Using MEMS technology, an ＂Au, Pt, Pt＂ three-microelectrode system enclosed in a SU-8 micro pool was fabricated. Employing SAMs, a monolayer of protein A was immobilized on the cysteamine modified Au electrode to achieve the orientation-controlled immobilization of the human immunoglobulin （HIgG） antibody. The immunosensor aimed at low unit cost, small dimension, high level of integration and the prospect of a biosensor system-on-a-chip. Cyclic voltammetry and chronoamperometry were conducted to characterize the immunosensor. Compared with the traditional immunosensor using bulky gold electrode or screen-printed electrode and the procedure directly binding protein A to electrode for immobilization of antibodies, it had attractive advantages, such as miniaturization, compatibility with CMOS technology, fast response （30 s）, broad linear range （50-400 pg/L） and low detection limit （10 pg/L） for HIgG. In addition, this immunosensor was easy to be designed into micro array and to realize the simultaneously multi-parameter detection.
文摘该文实现了一种基于电控旋转磁场的电磁跟踪系统。设计了以数字信号处理器(Digital Signal Processor,DSP)作为控制处理装置,并且包括一个可控恒流源模块、一个磁场源模块、一个三轴磁传感器及ADC接口电路的电磁跟踪系统。初步的实验测试结果表明系统每次都能稳定实现定位,平均位置误差为0.282 cm,平均姿态误差为0.696°,定位时间为1.572 s。通过标定、校准以及硬件电路的进一步改进,该系统的性能有望进一步提高。
