This work has been undertaken to study the occurrence of Clostridium perfringens contamination in the poultry feed ingredients and find out its in-vitro antibiotic sensitivity pattern to various antimicrobial drugs. T...This work has been undertaken to study the occurrence of Clostridium perfringens contamination in the poultry feed ingredients and find out its in-vitro antibiotic sensitivity pattern to various antimicrobial drugs. Two hundred and ninety-eight poultry feed ingredient samples received at Poultry Disease Diagnosis and Surveillance Laboratory, Namakkal, Tamil Nadu in South India were screened for the presence of C. perfringens. The organisms were isolated in Perfringens agar under anaerobic condition and subjected to standard biochemical tests for confirmation. In vitro antibiogram assay has been carried out to determine the sensitivity pattern of the isolates to various antimicrobial drugs. One hundred and one isolates of C. perfringens were obtained from a total of 298 poultry feed ingredient samples. Overall positivity of 33.89% could be made from the poultry feed ingredients. Highest level of C. perfringens contamination was detected in fish meal followed by bone meal, meat and bone meal and dry fish.Antibiogram assay indicated that the organisms are highly sensitive to gentamicin(100%), chlortetracycline(96.67%), gatifloxacin(93.33%), ciprofloxacin(86.67%), ofloxacin(86.67%) and lincomycin(86.67%). All the isolates were resistant to penicillin-G. Feed ingredients rich in animal proteins are the major source of C. perfringens contamination.展开更多
真姬菇(Hypsizygus marmoreus)凭借独特的风味和优良的保健功效成为工厂化栽培的食用菌之一,但生产过程中易受杂菌污染,为了分析污染原因,降低污染率和生产成本,采用稀释涂平板法从菌袋上部变黄吐黄水的污染菌袋中分离得到5株细菌及5株...真姬菇(Hypsizygus marmoreus)凭借独特的风味和优良的保健功效成为工厂化栽培的食用菌之一,但生产过程中易受杂菌污染,为了分析污染原因,降低污染率和生产成本,采用稀释涂平板法从菌袋上部变黄吐黄水的污染菌袋中分离得到5株细菌及5株真菌,通过形态学结合细菌16S r RNA以及真菌ITS保守序列进行鉴定并构建了系统发育树。研究结果表明,5株细菌分别属于芽孢杆菌属(Bacillus)、肠杆菌科(Enterobacter)、西地西菌属(Cedecea)、假单胞菌属(Pseudomonas)、藤黄色杆菌属(Luteibacter),5株真菌分别属于枝孢菌属(Cladosporium)、青霉菌属(Penicillium)、曲霉菌属(Aspergillus)、格孢腔菌目(Pleosporales)、镰孢菌属(Fusarium)。系统发育树分析表明污染菌相互之间的亲缘关系较远。展开更多
为加强石油污染土壤治理,加大相关降解菌资源的挖掘和筛选,研究采集南疆干旱区域石油污染土壤3份,采用石油为唯一碳源的无机盐培养基,通过富集和分离筛选,共获得63株潜在石油烃降解菌。经过16S r DNA测序鉴定,确定菌株共涉及厚壁菌门(Fi...为加强石油污染土壤治理,加大相关降解菌资源的挖掘和筛选,研究采集南疆干旱区域石油污染土壤3份,采用石油为唯一碳源的无机盐培养基,通过富集和分离筛选,共获得63株潜在石油烃降解菌。经过16S r DNA测序鉴定,确定菌株共涉及厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)等菌门的23个属,其中假单胞菌属(Pseudomonas)、微杆菌属(Microbacterium)、短芽胞杆菌属(Brevibacillus sp.)分别为2个种,涉及种类最多,其它均为1个种;在数量上,假单胞菌属10株,微杆菌属(Microbacterium)8株,假黄色单胞菌属(Psuedoxanthomonas)7株,苍白好氧小杆菌属(Aeribacillus)、土芽孢杆菌属(Geobacillus)4株,短芽孢杆菌属(Brevibacillus)、根瘤菌属(Rhizobium)、新鞘脂菌属(Novosphingobium)均为3株,沙壤土杆菌(Ramlibacter)、短杆菌属(Brevibacterium)、玫瑰单胞菌属(Roseomonas)、水杆菌属(Aquabacterium)、假根瘤菌属(Pseudorhizobium)、荒漠副根瘤菌属(paramesorhizobium)均为2株,其余均为1株,本次筛选菌株为进一步筛选高效石油降解菌提供了丰富研究材料。展开更多
文摘This work has been undertaken to study the occurrence of Clostridium perfringens contamination in the poultry feed ingredients and find out its in-vitro antibiotic sensitivity pattern to various antimicrobial drugs. Two hundred and ninety-eight poultry feed ingredient samples received at Poultry Disease Diagnosis and Surveillance Laboratory, Namakkal, Tamil Nadu in South India were screened for the presence of C. perfringens. The organisms were isolated in Perfringens agar under anaerobic condition and subjected to standard biochemical tests for confirmation. In vitro antibiogram assay has been carried out to determine the sensitivity pattern of the isolates to various antimicrobial drugs. One hundred and one isolates of C. perfringens were obtained from a total of 298 poultry feed ingredient samples. Overall positivity of 33.89% could be made from the poultry feed ingredients. Highest level of C. perfringens contamination was detected in fish meal followed by bone meal, meat and bone meal and dry fish.Antibiogram assay indicated that the organisms are highly sensitive to gentamicin(100%), chlortetracycline(96.67%), gatifloxacin(93.33%), ciprofloxacin(86.67%), ofloxacin(86.67%) and lincomycin(86.67%). All the isolates were resistant to penicillin-G. Feed ingredients rich in animal proteins are the major source of C. perfringens contamination.
文摘真姬菇(Hypsizygus marmoreus)凭借独特的风味和优良的保健功效成为工厂化栽培的食用菌之一,但生产过程中易受杂菌污染,为了分析污染原因,降低污染率和生产成本,采用稀释涂平板法从菌袋上部变黄吐黄水的污染菌袋中分离得到5株细菌及5株真菌,通过形态学结合细菌16S r RNA以及真菌ITS保守序列进行鉴定并构建了系统发育树。研究结果表明,5株细菌分别属于芽孢杆菌属(Bacillus)、肠杆菌科(Enterobacter)、西地西菌属(Cedecea)、假单胞菌属(Pseudomonas)、藤黄色杆菌属(Luteibacter),5株真菌分别属于枝孢菌属(Cladosporium)、青霉菌属(Penicillium)、曲霉菌属(Aspergillus)、格孢腔菌目(Pleosporales)、镰孢菌属(Fusarium)。系统发育树分析表明污染菌相互之间的亲缘关系较远。
文摘为加强石油污染土壤治理,加大相关降解菌资源的挖掘和筛选,研究采集南疆干旱区域石油污染土壤3份,采用石油为唯一碳源的无机盐培养基,通过富集和分离筛选,共获得63株潜在石油烃降解菌。经过16S r DNA测序鉴定,确定菌株共涉及厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)等菌门的23个属,其中假单胞菌属(Pseudomonas)、微杆菌属(Microbacterium)、短芽胞杆菌属(Brevibacillus sp.)分别为2个种,涉及种类最多,其它均为1个种;在数量上,假单胞菌属10株,微杆菌属(Microbacterium)8株,假黄色单胞菌属(Psuedoxanthomonas)7株,苍白好氧小杆菌属(Aeribacillus)、土芽孢杆菌属(Geobacillus)4株,短芽孢杆菌属(Brevibacillus)、根瘤菌属(Rhizobium)、新鞘脂菌属(Novosphingobium)均为3株,沙壤土杆菌(Ramlibacter)、短杆菌属(Brevibacterium)、玫瑰单胞菌属(Roseomonas)、水杆菌属(Aquabacterium)、假根瘤菌属(Pseudorhizobium)、荒漠副根瘤菌属(paramesorhizobium)均为2株,其余均为1株,本次筛选菌株为进一步筛选高效石油降解菌提供了丰富研究材料。