为了解新疆阿勒泰地区额尔齐斯河流域野生冷水鱼肠道微生物多样性及其群落结构组成。采用NGS高通量测序技术,分析3种冷水鱼肠道内容物和黏膜中细菌16S r RNA V4区基因序列,进而比较不同种类冷水鱼肠道内容物和黏膜中微生物的群落结构组...为了解新疆阿勒泰地区额尔齐斯河流域野生冷水鱼肠道微生物多样性及其群落结构组成。采用NGS高通量测序技术,分析3种冷水鱼肠道内容物和黏膜中细菌16S r RNA V4区基因序列,进而比较不同种类冷水鱼肠道内容物和黏膜中微生物的群落结构组成及多样性差异。结果共获得58 328条有效序列,242个OTU。多样性分析表明,哲罗鱼样品中的Shannon指数明显低于细鳞鱼和黑斑狗鱼,细鳞鱼和黑斑狗鱼在群落结构上更为接近。在门水平6个样品中的菌群均以厚壁菌门(Firmicutes)、丙型变形菌门(Gammaproteobacteria)为主;哲罗鱼中Firmicutes是最占优势的菌群门,而细鳞鱼和黑斑狗鱼中Gammaproteobacteria显著增加,Firmicutes显著减少。属水平上哲罗鱼中主要以肉杆菌属(Carnobacterium)为主,细鳞鱼和黑斑狗鱼中嗜冷杆菌属(Psychrobacter)、不动杆菌属(Acinetobacter)、梭菌属(Clostridium sensu stricto)等比例较高。不同种鱼肠道微生物的组成及多样性在门和属水平上有明显差异,而同种冷水鱼的肠道内容物和黏膜间微生物群落组成较为相似,无显著差异。展开更多
This article first analyzes the status quo of industrial development of cold water fishes in Henan Province. And then it points out the main problems in the process of cold water fish industrialization as follows: the...This article first analyzes the status quo of industrial development of cold water fishes in Henan Province. And then it points out the main problems in the process of cold water fish industrialization as follows: the parent fish rearing of cold water fishes and the supply of roe and fry are inadequate; the demand for feed nutrition,research and development of disease prevention and control technology lag behind. In order to provide scientific basis for promoting the industrial independent innovation and international competitiveness,finally the following ideas and recommendations for industrial development of cold water fishes are put forward: strengthening policy support for the enterprises,and striving to create the leading enterprises,to achieve dramatic development; implementing the strategy of developing fishery by relying on science and technology,promoting the industrial independent innovation and competitiveness; creating specialized cooperative organizations,actively improving the supporting system,and expanding the space for development of the industry.展开更多
文摘为了解新疆阿勒泰地区额尔齐斯河流域野生冷水鱼肠道微生物多样性及其群落结构组成。采用NGS高通量测序技术,分析3种冷水鱼肠道内容物和黏膜中细菌16S r RNA V4区基因序列,进而比较不同种类冷水鱼肠道内容物和黏膜中微生物的群落结构组成及多样性差异。结果共获得58 328条有效序列,242个OTU。多样性分析表明,哲罗鱼样品中的Shannon指数明显低于细鳞鱼和黑斑狗鱼,细鳞鱼和黑斑狗鱼在群落结构上更为接近。在门水平6个样品中的菌群均以厚壁菌门(Firmicutes)、丙型变形菌门(Gammaproteobacteria)为主;哲罗鱼中Firmicutes是最占优势的菌群门,而细鳞鱼和黑斑狗鱼中Gammaproteobacteria显著增加,Firmicutes显著减少。属水平上哲罗鱼中主要以肉杆菌属(Carnobacterium)为主,细鳞鱼和黑斑狗鱼中嗜冷杆菌属(Psychrobacter)、不动杆菌属(Acinetobacter)、梭菌属(Clostridium sensu stricto)等比例较高。不同种鱼肠道微生物的组成及多样性在门和属水平上有明显差异,而同种冷水鱼的肠道内容物和黏膜间微生物群落组成较为相似,无显著差异。
基金Supported by Special Fund for Agro-scientific Research in the Public Interest ( 201003055)
文摘This article first analyzes the status quo of industrial development of cold water fishes in Henan Province. And then it points out the main problems in the process of cold water fish industrialization as follows: the parent fish rearing of cold water fishes and the supply of roe and fry are inadequate; the demand for feed nutrition,research and development of disease prevention and control technology lag behind. In order to provide scientific basis for promoting the industrial independent innovation and international competitiveness,finally the following ideas and recommendations for industrial development of cold water fishes are put forward: strengthening policy support for the enterprises,and striving to create the leading enterprises,to achieve dramatic development; implementing the strategy of developing fishery by relying on science and technology,promoting the industrial independent innovation and competitiveness; creating specialized cooperative organizations,actively improving the supporting system,and expanding the space for development of the industry.
