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Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth 被引量:8
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作者 Stephen F. Garczynski Brad S. Coates +4 位作者 Thomas R. Unruh Scott Schaeffer Derick Jiwan Tyson Koepke Amit Dhingra 《Insect Science》 SCIE CAS CSCD 2013年第5期559-574,共16页
The codling moth, Cydia pomonella, is one of the most important pests of pome fruits in the world, yet the molecular genetics and the physiology of this insect remain poorly understood. A combined assembly of 8?341 e... The codling moth, Cydia pomonella, is one of the most important pests of pome fruits in the world, yet the molecular genetics and the physiology of this insect remain poorly understood. A combined assembly of 8?341 expressed sequence tags was generated from Roche 454 GS-FLX sequencing of eight tissue-specific cDNA libraries. Putative chemosensory proteins (12) and odorant binding proteins (OBPs) (18) were annotated, which included three putative general OBP (GOBP), one more than typically reported for other Lepidoptera. To further characterize CpomGOBPs, we cloned cDNA copies of their transcripts and determined their expression patterns in various tissues. Cloning and sequencing of the 698?nt transcript for CpomGOBP1 resulted in the prediction of a 163 amino acid coding region, and subsequent RT-PCR indicated that the transcripts were mainly expressed in antennae and mouthparts. The 1?289 nt (160 amino acid) CpomGOBP2 and the novel 702 nt (169 amino acid) CpomGOBP3 transcripts are mainly expressed in antennae, mouthparts, and female abdomen tips. These results indicate that next generation sequencing is useful for the identification of novel transcripts of interest, and that codling moth expresses a transcript encoding for a new member of the GOBP subfamily. 展开更多
关键词 codling moth EST odorant binding proteins
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Comparison of fatty acid composition in total lipid of diapause and non-diapause larvae of Cydia pomonella (Lepidoptera: Tortricidae) 被引量:6
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作者 ABBAS KHANI SAEID MOHARRAMIPOUR +1 位作者 MOHSEN BARZEGAR HOSSEIN NADERI-MANESH 《Insect Science》 SCIE CAS CSCD 2007年第2期125-131,共7页
Seasonal changes in the fatty acid composition of the total lipid extracted from the whole body of Cydia pomonella L. larvae were determined by gas chromatography. The six most abundant fatty acids in both non-diapaus... Seasonal changes in the fatty acid composition of the total lipid extracted from the whole body of Cydia pomonella L. larvae were determined by gas chromatography. The six most abundant fatty acids in both non-diapause and diapause larvae of codling moth were oleic (35%-39%), palmitic (23%-33%), linoleic (16%-30%), palmitoleic (5%-10%), stearic (1.5%-3.0%) and linolenic acids (1.0%-2.5%). This represents a typical complement of Lepidopteran fatty acids. The fatty acid composition of total lipid of C. pomonella larvae was related to diapause. In similarity to most other reports, the proportion of unsaturated fatty acids increased in diapause initiation state. The total lipid of diapause larvae contained more linoleic acid (25.8% vs. 16.1%) and less palmitic acid (24.7% vs. 33.4%), than that of non-diapause larvae. The weight percentage of linoleic acid (C 18:2) increased from 16% to 26% from early-August through early-September during transition to diapause, while palmitic acid (C16:0) decreased from 33% to 25% at the same time. These changes resulted in an increase in the ratio of unsaturated to saturated fatty acids (UFA/SFA) from 1.72 in non-diapause larvae to 2.63 in diapause larvae. 展开更多
关键词 codling moth Cydia pomonella DIAPAUSE fatty acid composition LIPID
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Putative nicotinic acetylcholine receptor subunits express differentially through the life cycle of codling moth, Cydia pomonella (Lepidoptera: Tortricidae) 被引量:3
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作者 Jessica A. Martin Stephen F. Garczynski 《Insect Science》 SCIE CAS CSCD 2016年第2期277-287,共11页
Nicotinic acetylcholine receptors (nAChRs) are the targets of neonicotinoids and spinosads, two insecticides used in orchards to effectively control codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae)... Nicotinic acetylcholine receptors (nAChRs) are the targets of neonicotinoids and spinosads, two insecticides used in orchards to effectively control codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). Orchardists in Washington State are concerned about the possibility of codling moth field populations developing resistance to these two insecticides. In an effort to help mitigate this issue, we initiated a project to identify and characterize codling moth nAChR subunits expressed in heads. This study had two main goals; (i) identify transcripts from a codling moth head transcriptome that encode for nAChR subunits, and (ii) determine nAChR subunit expression profiles in various life stages of codling moth. From a codling moth head transcriptome, 24 transcripts encoding for 12 putative nAChR subunit classes were identified and verified by PCR amplification, cloning, and sequence determination. Characterization of the deduced protein sequences encoded by putative nAChR transcripts revealed that they share the distinguishing features of the cys-loop ligand-gated ion channel superfamily with 9 α-type subunits and 3 β- type subunits identified. Phylogenetic analysis comparing these protein sequences to those of other insect nAChR subunits supports the identification of these proteins as nAChR subunits. Stage expression studies determined that there is clear differential expression of many of these subunits throughout the codling moth life cycle. The information from this study will be used in the future to monitor for potential target-site resistance mechanisms to neonicotinoids and spinosads in tolerant codling moth populations. 展开更多
关键词 codling moth nAChR expression nicotinic acetylcholine receptor subunits
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Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults 被引量:1
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作者 Laura Beatriz Parra Morales Raul Adolfo Alzogaray +3 位作者 Liliana Cichon Silvina Garrido Jimena Soleno Cristina Monica Montagna 《Insect Science》 SCIE CAS CSCD 2017年第3期455-466,共12页
The control program of codling moth (Cydia pomonella L.) in the Rio Negro and Neuqu6n Valley is intended to neonate larvae. However, adults may be subjected to sublethal pesticide concentrations generating stress wh... The control program of codling moth (Cydia pomonella L.) in the Rio Negro and Neuqu6n Valley is intended to neonate larvae. However, adults may be subjected to sublethal pesticide concentrations generating stress which might enhance both mutation rates and activity of the detoxification system. This study assessed the exposure effects of chlorpyrifos on target enzyme and, both detoxifying and antioxidant systems of surviving adults from both a laboratory susceptible strain (LSS) and a field population (FP). The results showed that the FP was as susceptible to chlorpyrifos as the LSS and, both exhibited a similar chlorpyrifos-inhibitory concentration 50 (IC50) of acetylcholinesterase (ACHE). The FP displayed higher carboxylesterase (CarE) and 7-ethoxycoumarine O-deethylase (ECOD) activities than LSS. Both LSS and FP showed an increase on CarE activity after the exposure to low-chlorpyrifos concentrations, followed by enzyme inhibition at higher concentrations. There were no significant differences neither in the activities of glutathione S-transferases (GST), catalase (CAT) and superoxide dismutase (SOD) nor in the reduced glutathione (GSH) content between LSS and FP. Moreover, these enzymes were unaffected by chlorpyrifos. In conclusion, control adults from the FP exhibited higher CarE and ECOD activities than control adults from the LSS. AChE and CarE activities were the most affected by chlorpyrifos. Control strategies used for C. pomonella, such as rotations of insecticides with different modes of action, will probably delay the evolution of insecticide resistance in FPs from the study area. 展开更多
关键词 ACETYLCHOLINESTERASE antioxidant system CHLORPYRIFOS codling moth detoxification enzymes
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Identification and developmental expression of putative gene encoding juvenile hormone esterase(CpJHE-like) in codling moth, Cydia pomonella(L.) 被引量:1
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作者 HUANG Cong WU Qiang +8 位作者 JIANG Chun-yan XING Long-sheng SHI Guo-liang ZHANG Bin QIAN Wan-qiang LI You-zhi XI Yu YANG Nian-wan WAN Fang-hao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第7期1624-1633,共10页
Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(Cp... Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data.The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE).CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE).Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae.The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva,increased about 300-fold compared to the basal level.Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body.This study indicates that the CpJHE-like is the functional JHE,which may play vital roles in the development and reproduction of C.pomonella. 展开更多
关键词 JUVENILE HORMONE ESTERASE CpJHE-like codling MOTH Cydia pomonella
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Thin-Plate Spline Analysis of Diversity in Field Populations of Codling Moth, Cydia pomonella L. (Lep., Tortricidae) in North West of Iran
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作者 S. Khaghaninia S. A. Mohammadi +2 位作者 A. M. Sarafrazi K. Haddad Irani Nejad R. Zahiri 《Journal of Agricultural Science and Technology》 2011年第1期35-41,共7页
In order to study morphological diversity of codling moth, Cydia pomonella (L.) using thin-plate spline analysis, nine geographical populations from four north western provinces of Iran namely East Azarbayjan, West ... In order to study morphological diversity of codling moth, Cydia pomonella (L.) using thin-plate spline analysis, nine geographical populations from four north western provinces of Iran namely East Azarbayjan, West Azarbayjan, Ardebil and Zandjan were collected during 2003 and 2004. 575 and 564 images were prepared from fore and hind wings, respectively. Then 15 and 11 landmarks were determined from fore and hind wings, respectively. With transforming of landmark's two dimensional coordinate data into partial warp scores, 26 and 18 scores were generated for fore and hind wings, respectively. Cluster analysis based on wing shape variables using Ward's algorithm assigned nine geographical populations into two groups. The pattern of grouping based on fore and hind wings was different in both sexes. Principal component analysis revealed discrimination between geographic populations and confirmed the result of cluster analysis. Among environmental parameters, wind speed showed the highest correlation with wing shape variables. Non significant correlation was observed between geographic and morphological distance matrices as revealed by Mantel test. 展开更多
关键词 Geometric morphometrics population variation thin-plate spline analysis codling moth Cydia pomonella (L.)
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PREMISE Insect Model: Integrated Population Dynamics Model for the Ex-ante Evaluation of IPM against Insect Pest
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作者 Wil Hennen Aude Alaphilippe 《Journal of Agricultural Science and Technology(B)》 2015年第4期231-240,共10页
Codling moth Cydiapomonella L. is the most serious pest of apple and pear worldwide and causes damage and decreased yields. To minimize this risk, IPM tools can be applied to reduce the use of chemicals. A cost-effect... Codling moth Cydiapomonella L. is the most serious pest of apple and pear worldwide and causes damage and decreased yields. To minimize this risk, IPM tools can be applied to reduce the use of chemicals. A cost-effective application of IPM depends on the number of insects at the time of application. Several conditions and factors influence the lifecycle and numbers of generations within a year. In order to perform ex-ante evaluations of the cost-effectiveness of IPM measures against pest insects, the generic PREMISE insect model has been developed, which integrates insect's population dynamics, the climate and other conditions, applied measures, economics, environment and human health (residues). This paper describes these components of the PREMISE insect model, and how these components interact to assess the cost-effectiveness of IPM measures and the effects on the environment and human health. Codling moth is used as a case study for this generic model. 展开更多
关键词 IPM integrated model population dynamics ECONOMICS environment RESIDUES codling moth.
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Field Trials of CpGV Virus Isolates Overcoming Resistance to CpGV-M
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作者 M. Berling J. -B. Rey +5 位作者 S. -J. Ondet Y. Tallot O. Soubabère A. Bonhomme B. Sauphanor M. Lopez-Ferber 《Virologica Sinica》 SCIE CAS CSCD 2009年第5期470-477,共8页
The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France... The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations. 展开更多
关键词 Cydia pomonella codling moth Biological control Genotype selection Field trials
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两种苹果蠹蛾性引诱剂诱捕器诱捕效率比较及地面植被的影响 被引量:36
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作者 杜磊 张润志 +1 位作者 蒲崇建 贾迎春 《昆虫知识》 CSCD 北大核心 2007年第2期233-237,共5页
在甘肃省高台县通过对三角胶粘式和水盆式这2种类型苹果蠹蛾Cydia pomonella(L.)诱捕器的田间比较研究。结果显示,二者在诱捕效率上存在的明显差异。在苹果蠹蛾密度较低的条件下,三角胶粘式诱捕器平均日诱捕量为2.50只,比水盆式诱捕... 在甘肃省高台县通过对三角胶粘式和水盆式这2种类型苹果蠹蛾Cydia pomonella(L.)诱捕器的田间比较研究。结果显示,二者在诱捕效率上存在的明显差异。在苹果蠹蛾密度较低的条件下,三角胶粘式诱捕器平均日诱捕量为2.50只,比水盆式诱捕器高出近2.84倍,最早监测到成虫的时间也比水盆式诱捕器提前3-4d,因此,三角胶粘式诱捕器具有更高的监测效率。结果还显示地面植被的遮盖极大地降低诱捕器的诱捕效果,严重时会使诱捕器对密度较低的苹果蠹蛾种群的监测功能丧失。 展开更多
关键词 苹果蠹蛾 cydia pomonella 性诱剂诱捕器 植被 监测
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3种性诱捕器诱捕苹果蠹蛾效果比较及成虫的时序动态变化 被引量:20
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作者 石磊 陈明 罗进仓 《甘肃农业大学学报》 CAS CSCD 北大核心 2009年第1期115-117,137,共4页
通过对3种苹果蠹蛾性诱捕器诱捕效果的比较,发现板式性诱捕器的诱捕效果最好,盆式与瓶式之间无差异;对苹果蠹蛾成虫综合防治果园和未防治果园中的时序变化以及防治措施对成虫动态变化的研究发现,在综合防治果园中苹果蠹蛾成虫的数量明... 通过对3种苹果蠹蛾性诱捕器诱捕效果的比较,发现板式性诱捕器的诱捕效果最好,盆式与瓶式之间无差异;对苹果蠹蛾成虫综合防治果园和未防治果园中的时序变化以及防治措施对成虫动态变化的研究发现,在综合防治果园中苹果蠹蛾成虫的数量明显降低,第1代和第2代成虫每单位诱捕器3 d内诱捕虫头数在2头以下,盛发期前后的2次化学防治为越冬代成虫防治的关健时期. 展开更多
关键词 苹果蠹蛾 性诱捕器 成虫时序动态
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苹果蠹蛾疫情防控阻截动态及思考 被引量:20
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作者 王春林 王福祥 《植物保护》 CAS CSCD 北大核心 2009年第2期102-104,共3页
苹果蠹蛾近年来正从甘肃河西走廊向内地蔓延,形势十分严峻。甘肃省从保护本省苹果生产大局出发,自2006年以来采取一系列措施阻截苹果蠹蛾,全省设立了1300多个疫情监测点,开展了疫情普查,划定了疫区,设立了10个公路检查站,开展了防控行动... 苹果蠹蛾近年来正从甘肃河西走廊向内地蔓延,形势十分严峻。甘肃省从保护本省苹果生产大局出发,自2006年以来采取一系列措施阻截苹果蠹蛾,全省设立了1300多个疫情监测点,开展了疫情普查,划定了疫区,设立了10个公路检查站,开展了防控行动,砍伐废弃果园,取得了明显的效果。但是,目前疫情控制工作仍面临一些突出的问题,如疫情人为传播严重、防控资金缺口较大、监测防控能力不足等。建议从4个方面加强今后的疫情防控工作:一是加强部省行政领导;二是加大各级对疫情防控的资金投入;三是强化疫情阻截和防控措施;四是进一步开展防控技术研究,提高检疫控制的效果。 展开更多
关键词 苹果蠹蛾 阻截 控制
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检疫性危险害虫苹果蠹蛾在我国分布的调查研究 被引量:16
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作者 金瑞华 《中国科学基金》 CSCD 1997年第2期124-125,共2页
苹果蠢蛾[Cydia Pomonella(L.)]是世界上分布最广、为害最重的苹果、梨、桃、核桃等仁果类、核果类水果的毁灭性害虫。
关键词 苹果蠹蛾 中国 分布 调查研究 果树 害虫 水果 检疫
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查清我国东部地区无苹果蠹蛾发生 被引量:15
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作者 全国苹果蠹蛾研究协作组 《植物保护学报》 CAS CSCD 北大核心 1994年第2期169-175,共7页
苹果蠹蛾[Laspeyresia pomonella(L.)]是世界检疫性害虫。英联邦农业局(CAB)受联合国粮农组织(FAO)的委托,多次公布的该虫世界分布图上,一再将我国东部(尤其渤海湾沿岸)划为该虫分布区,从而严重影响我国苹果、梨的出口;同时,一些有该虫... 苹果蠹蛾[Laspeyresia pomonella(L.)]是世界检疫性害虫。英联邦农业局(CAB)受联合国粮农组织(FAO)的委托,多次公布的该虫世界分布图上,一再将我国东部(尤其渤海湾沿岸)划为该虫分布区,从而严重影响我国苹果、梨的出口;同时,一些有该虫分布的国家也据此要求我国放宽对苹果进口的检疫规定,构成了对我国苹果、梨生产的严重威胁。为查清该虫在我国的分布实况,作者在1991年监测调查的基础上,于1992—1993年,在辽、鲁、冀、京、豫五省市进行了多点重复监测调查。3年来,各基点于4—10月进行该虫性诱剂诱集监测,监测面积累计达648.2ha,代表不同地区不同类型果园;于7—10月进行果园调查及剖果检查,共计44次,调查了77县(区)的176个果园,累计调查面积达5058.9ha,共抽查了15163株果树的1978419个果实。结果在上述地区连续三年未发现该虫。结合对CAB图依据文献的核查,发现其错误引用丁资料。因此,本文论证了我国东部地区无苹果蠹蛾的分布,CAB图应予更正。 展开更多
关键词 苹果 囊蛾 中国东部地区 果园调查
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7种药剂对苹果蠹蛾的防治效果 被引量:17
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作者 杨建强 赵骁 +2 位作者 严勇敢 张雅林 冯纪年 《西北农业学报》 CAS CSCD 北大核心 2011年第9期194-196,共3页
为了筛选对苹果蠹蛾毒力大、防效好的生物药剂,为其防治提供依据,选用7种药剂进行室内毒力测定和田间药效试验。结果表明,5%甲氨基阿维菌素苯甲酸盐GR1 000倍液和8 000 IU/mg苏云金芽孢杆菌SP1 000倍液的防效在药后30 d均达到80%以上,... 为了筛选对苹果蠹蛾毒力大、防效好的生物药剂,为其防治提供依据,选用7种药剂进行室内毒力测定和田间药效试验。结果表明,5%甲氨基阿维菌素苯甲酸盐GR1 000倍液和8 000 IU/mg苏云金芽孢杆菌SP1 000倍液的防效在药后30 d均达到80%以上,且不污染环境,正常施药后不产生药害。因此,这两种药剂适合在防治苹果蠹蛾时大面积推广使用。 展开更多
关键词 苹果蠹蛾 毒力测定 药效试验 致死中量
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苹果蠹蛾在中国的扩散与危害 被引量:16
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作者 徐婧 刘伟 +2 位作者 刘慧 吴立峰 张润志 《生物安全学报》 2015年第4期327-336,共10页
【背景】苹果蠹蛾原产于欧亚大陆,是世界著名的严重危害苹果生产的入侵害虫,也是中国的重要对外检疫性有害生物。【方法】通过收集整理历史文献、资料,并于2005~2013年使用标准性信息素诱芯对全国各苹果、梨主产区的苹果蠹蛾发生情况进... 【背景】苹果蠹蛾原产于欧亚大陆,是世界著名的严重危害苹果生产的入侵害虫,也是中国的重要对外检疫性有害生物。【方法】通过收集整理历史文献、资料,并于2005~2013年使用标准性信息素诱芯对全国各苹果、梨主产区的苹果蠹蛾发生情况进行系统监测,明确该虫在我国的分布现状及扩散历史;此外,使用特定公式对苹果蠹蛾在发生区造成的当前经济损失和扩散完成后的潜在经济损失进行了系统评估,以明确该虫在我国的现有和潜在危害。【结果】苹果蠹蛾于1953年首次在中国新疆被发现,目前在中国分布于新疆、甘肃、内蒙古、宁夏、黑龙江、辽宁和吉林7省的144个县(市、区、旗),主要集中分布在东经74.56°~106.8°以及东经120.02°~132.95°,发生面积约49410 hm^2;经济损失评估结果显示,苹果蠹蛾导致中国苹果和梨的潜在减产数量为185.01万和81.33万t。【结论与意义】该虫已在中国形成东西2个分布区,对占据中国苹果产量80%的西北黄土高原(陕西为主)和渤海湾(山东、河北、辽宁为主)两大苹果主产区构成了严重威胁,须引起高度重视。 展开更多
关键词 苹果蠹蛾 入侵生物 中国 分布 扩散 经济损失
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苹果蠹蛾性信息素田间应用技术研究 被引量:13
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作者 张涛 赵江华 +1 位作者 冯俊涛 张兴 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2011年第5期167-171,178,共6页
【目的】探讨苹果蠹蛾性信息素田间应用中的技术问题,为苹果蠹蛾性信息素的科学应用提供理论依据。【方法】采用三角式、飞翼式、水盆式和圆筒式4种诱捕器,较系统地研究了诱捕器类型和悬挂高度、苹果蠹蛾性信息素剂量和纯度等因素对诱... 【目的】探讨苹果蠹蛾性信息素田间应用中的技术问题,为苹果蠹蛾性信息素的科学应用提供理论依据。【方法】采用三角式、飞翼式、水盆式和圆筒式4种诱捕器,较系统地研究了诱捕器类型和悬挂高度、苹果蠹蛾性信息素剂量和纯度等因素对诱捕效果的影响。【结果】三角式和飞翼式诱捕器的诱捕效果较佳;诱捕器设置在树冠中上部的诱捕量极显著高于下部及顶部;每诱芯中苹果蠹蛾性信息素含量为0.75 mg时诱捕效果最佳,剂量低于每诱芯0.25 mg或高于1.5 mg时,诱捕量显著下降;性信息素纯度对诱捕效果影响较大,在每诱芯1.00 mg剂量下,纯度为75%的性信息素诱捕效果较佳。【结论】田间宜采用三角式粘胶诱捕器;每诱芯中含0.75 mg苹果蠹蛾性信息素处理的诱捕效果最佳;性信息素纯度不低于75%即可达到制备诱芯的要求;诱捕器设置在树冠中上部诱蛾效果更好。 展开更多
关键词 苹果蠹蛾 性信息素 诱捕器类型 悬挂高度 诱捕效果
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苹果蠹蛾空间分布型及数学消长模型研究 被引量:9
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作者 林伟丽 于江南 薛光华 《新疆农业大学学报》 CAS 2007年第1期40-43,共4页
为进一步了解苹果蠹蛾(Cydia pomonellaL.)的生态学习性,对该虫在香梨园的空间分布型及其数学消长模型进行了研究。结果表明:苹果蠹蛾在田间的空间分布型受密度的影响较大:成虫在平均密度m≤3时,个体间相互吸引,分布的基本成分为个体群... 为进一步了解苹果蠹蛾(Cydia pomonellaL.)的生态学习性,对该虫在香梨园的空间分布型及其数学消长模型进行了研究。结果表明:苹果蠹蛾在田间的空间分布型受密度的影响较大:成虫在平均密度m≤3时,个体间相互吸引,分布的基本成分为个体群,呈聚集分布,且聚集强度随种群密度的升高而增加;在m>3时,个体间相互排斥,呈均匀分布;构建了苹果蠹蛾的数学消长模型:越冬代Nt=0.02669.t3.07095.e-0.26881t;第一代Nt=5.27497×10-7.t6.70833.e-0.24771t。 展开更多
关键词 香梨 苹果蠹蛾 空间分布型 数学模型
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生物源农药在苹果蠹蛾防治中的应用 被引量:10
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作者 吴正伟 杨雪清 张雅林 《生物安全学报》 2015年第4期299-305,共7页
苹果蠹蛾是世界性检疫害虫,对我国苹果优势产区构成了巨大威胁。长期依赖化学防治使该虫抗性问题变得十分严峻。为了保障食品安全,以环境友好的生物源农药替代化学农药已成为当前苹果蠹蛾防治的热点。本文对国内外现有的生物源农药,如... 苹果蠹蛾是世界性检疫害虫,对我国苹果优势产区构成了巨大威胁。长期依赖化学防治使该虫抗性问题变得十分严峻。为了保障食品安全,以环境友好的生物源农药替代化学农药已成为当前苹果蠹蛾防治的热点。本文对国内外现有的生物源农药,如寄生蜂、不育昆虫、颗粒体病毒、病原线虫、Bt、病原真菌、微孢子虫、性信息素、斑蝥素、多杀菌素等,在苹果蠹蛾防治中的最新应用及其存在的问题进行论述,讨论了生物源农药凭借其种类多、来源广且在用药时期上选择性强等特点,在该虫综合治理中的重要地位及面临的挑战。 展开更多
关键词 生物农药 苹果蠹蛾 生物防治 杀虫剂
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苹果蠹蛾在辽宁省彰武县梨树上的发生规律 被引量:10
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作者 房阳 蔡明 +3 位作者 可欣 邢竣策 杨雪清 王小奇 《植物保护学报》 CAS CSCD 北大核心 2018年第4期724-730,共7页
为明确辽宁省苹果蠹蛾Cydia pomonella(Linnaeus)的发生规律,于2014—2016年连续3年对辽宁省彰武县哈尔套镇10个村59个不同梨园采用三角屋胶粘式诱捕器进行了雄成虫数量消长动态的监测,并对幼虫蛀果率、老熟幼虫数量及化蛹行径、蛹期进... 为明确辽宁省苹果蠹蛾Cydia pomonella(Linnaeus)的发生规律,于2014—2016年连续3年对辽宁省彰武县哈尔套镇10个村59个不同梨园采用三角屋胶粘式诱捕器进行了雄成虫数量消长动态的监测,并对幼虫蛀果率、老熟幼虫数量及化蛹行径、蛹期进行调查。结果表明,在辽宁省彰武县,从4月下旬至9月下旬,苹果蠹蛾成虫共有2次发生高峰:第1次为5月下旬,第2次为7月中下旬;苹果蠹蛾在2016年全年内有3次明显的蛀果高峰期:第1次为6月下旬至7月上旬,第2次为8月上旬,第3次为8月下旬。老熟幼虫更趋向于脱果后直接掉落到地上再从地上沿着树干往上爬并在老翘皮下寻找合适位置化蛹;苹果蠹蛾经人工饲养的蛹期为9.95 d。研究表明,苹果蠹蛾在辽宁省彰武县1年发生2代,第1代从5月中旬到9月下旬,越冬代从7月中旬到翌年6月下旬。 展开更多
关键词 苹果蠹蛾 发生规律 综合治理
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苹果蠹蛾入侵的影响因素及检疫调控措施 被引量:10
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作者 蔡青年 赵欣 胡远 《中国农学通报》 CSCD 2007年第11期279-283,共5页
苹果蠹蛾是世界性重要的果树害虫之一,适应性广,危害严重,成为许多国家重要的检疫对象。为了更有效地防止苹果蠹蛾入侵中国主要水果产区,借鉴更安全环保的检疫处理和苹果蠹蛾发生区的控制措施,分析了苹果蠹蛾的传播途径和成功入侵的影... 苹果蠹蛾是世界性重要的果树害虫之一,适应性广,危害严重,成为许多国家重要的检疫对象。为了更有效地防止苹果蠹蛾入侵中国主要水果产区,借鉴更安全环保的检疫处理和苹果蠹蛾发生区的控制措施,分析了苹果蠹蛾的传播途径和成功入侵的影响因素如寄主植物、温度、湿度、降雨和光周期等,并重点介绍了溴甲烷熏蒸、低氧空气或混合气体处理和高温低氧低温冷藏处理等检疫处理,以及性诱剂、苹果蠹蛾颗粒病毒和利用赤眼蜂等田间生物防治措施。苹果蠹蛾的成功入侵与寄主植物的种类有直接的关系,而气候条件则是影响其种群的重要因素。3种检疫处理措施均能安全有效地杀灭水果中苹果蠹蛾的不同虫态,而田间生物防治措施则能有效地控制苹果蠹蛾成虫、卵和幼虫3个重要的虫态,从而降低水果的损失。因此,这些结果为苹果蠹蛾的内外检疫和和田间控制提供了重要的参考。 展开更多
关键词 苹果蠹蛾 入侵 检疫措施 防治措施
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