An investigation was carried out on living coccolithophores （LCs） distribution in the Yellow Sea and the East China Sea from October 17 to November 24, 2011. A total of 223 samples from different depths were col-lec...An investigation was carried out on living coccolithophores （LCs） distribution in the Yellow Sea and the East China Sea from October 17 to November 24, 2011. A total of 223 samples from different depths were col-lected at 48 stations. Totally 18 taxa belonging to coccolithophyceae were identified using a polarized micro-scope at the 1 000× magnification. The maximum species abundance was found at the outside of Transect P. The dominated species wereGephyrocapsa oceanica, Emiliania huxleyi,Helicosphaera carteri,andAlgiros-phaera robusta.The abundance of coccoliths and cells ranged 0-2 965.73 coccoliths/mL, and 0-119.16 cells/mL, with the average values of 471.00 coccoliths/mL and 23.42 cells/mL, respectively. The LCs in surface layer were mainly observed on the coastal belt and middle part of the survey area. The comparison among Transects A, F, P and E indicated lower species diversity and less abundance in the Yellow Sea than those of the East China Sea. The highest abundance of LCs was found in transect F and P. The coccolith abundance increased slightly from surface to bottom in the water column, but the highest value of the cell abundance was observed in the depth of 10-30 m. Temperature, depth and nutrient concentration were suggested as the major environmental factors controlling the distribution and species composition of LCs in the studying area based on canonical correspondence analysis （CCA）.展开更多
Analyses of the original and pyrolytic products of coccolithophore at various temperatures suggest that the contribution of coccolithophore to the formation of large immature oil reservoirs should attract keen interes...Analyses of the original and pyrolytic products of coccolithophore at various temperatures suggest that the contribution of coccolithophore to the formation of large immature oil reservoirs should attract keen interest. Through biochemical processes algae can change inorganic sulphur into organic sulphur, which could be one of the most important precursors of organic sulphur compounds in oil and source rocks. When Methylphenanthrene Index (MPI) and Methylphenanthrene Ratio (MPR) indices were used to evaluate the evolution degree of source rocks and oil maturity, other maturity indices must be used together for correction. In low maturity, the relative abundances of diben-zothiophene (DBT), fluorine (F) and dibenzofuran (DBF) can be used to identify the oxidation-reduction environments.展开更多
以北部湾1999年7月至2009年6月11年的Sea Wi FS卫星遥感颗粒无机碳度资料为基础,研究北部湾颗粒无机碳浓度的时空分布特征,并初步探讨其可能的海洋动力调控机制。结果表明:在季节变化上,整个冬季北部湾颗粒无机碳浓度普遍较高,表现出季...以北部湾1999年7月至2009年6月11年的Sea Wi FS卫星遥感颗粒无机碳度资料为基础,研究北部湾颗粒无机碳浓度的时空分布特征,并初步探讨其可能的海洋动力调控机制。结果表明:在季节变化上,整个冬季北部湾颗粒无机碳浓度普遍较高,表现出季节平均最大值(0.012 mol·m-3),春季普遍较低(<0.004 mol·m-3),表现出全年最低水平;不同区域的最高水平出现的季节却有所不同:北部湾北部沿岸区域颗粒无机碳浓度在秋季较高,海南岛西部大部分海域在冬季较高,北部湾西北部沿岸则在夏季较高;在空间分布上,近岸海区的颗粒无机碳浓度明显高于海湾中央区域,北部湾北部海域普遍高于南部海域,东部海域高于西部海域。综合相关分析表明,北部湾颗粒无机碳浓度的这一时空分布特征主要同季风的强弱及风向有关,同时也可能与海表温度、流场(如沿岸上升流等)及陆地径流量等有关。展开更多
Coccolith production is an important part of the biogenic carbon cycle as the largest source of Calcium carbonate on earth, accounting for about 75% of the deposition of carbon on the sea floor. Recent studies based o...Coccolith production is an important part of the biogenic carbon cycle as the largest source of Calcium carbonate on earth, accounting for about 75% of the deposition of carbon on the sea floor. Recent studies based on laboratory experiment results indicated that increasing anthropogenic CO2 in the atmosphere triggered global ocean acidification leading to a decrease of calcite or aragonite saturation and calcium carbonate, and to decreasing efficiency of carbon export/pumping to deep layers. In the present study, we analyzed about 20 years of field observations of coccolithophore pigment, dissolved inorganic carbon （DIC）, nutrients, and temperatures from the Bermuda Atlantic Time-series Study （BATS） site and satellite remote sensing to investigate the variable tendency of the coccolithophore pigment, and to evaluate the influence of ocean acidification on coccolithophore biomass. The results indicated that there was a generally increasing tendency of coccolithophore pigment, coupled with increasing bicarbonate concentrations or decreasing carbonate ion concentration. The change of coccolithophore pigment was also closely associated with pH, nutrients, mixed layer depth （MLD）, and temperature. Correlation analyses between coceolithophores and abiotic parameter imply that coccoliths production or coccolitho- phore pigment has increased with increasing acidification in the recent 20 years.展开更多
Rapid changes on nutrient supply and CO2 concentration that occurred in the northern South China Sea(SCS)during the Early Oligocene,provides an ideal natural laboratory,allowing us to peer into the coccolithophores’p...Rapid changes on nutrient supply and CO2 concentration that occurred in the northern South China Sea(SCS)during the Early Oligocene,provides an ideal natural laboratory,allowing us to peer into the coccolithophores’physiology in the geological records.In this study,we established a new nannofossil assemblage index,termed as E^*ratio,which is calculated by the relative abundance of eutrophic taxa and meso-oligotrophic taxa(E^*=e/e+c,where e is eutrophic taxa,and c is meso-oligotrophic taxa)Eutrophic taxa include small Reticulofenestra,Reticulofenestra lockeri group,Reticulofenestra bisecta group and Coccolithus pelagicus group,while meso-oligotrophic taxa include Cyclicargolithus spp.The E^*ratio is well correlated with nutrient proxy during the Early Oligocene,while with different covarying patterns under the higher and lower CO2 condition.By comparing the assemblage changes to the published data,we suggest that coccolithophores may change the way they use carbon source and nutrient with the decline of CO2.Furthermore,this implies a possible initiation of the carbon concentrating mechanism.展开更多
Pleurochrysis carterae is a calcifi ed coccolithophorid species that usually blooms in the coastal area and causes aquaculture losses. The cellular calcifi cation, blooming and many other critical species specifi c ec...Pleurochrysis carterae is a calcifi ed coccolithophorid species that usually blooms in the coastal area and causes aquaculture losses. The cellular calcifi cation, blooming and many other critical species specifi c eco-physiological processes are closely related to various metabolic pathways. The purpose of this study is to apply the unbiased and non-destructive method of nuclear magnetic resonance(NMR) to detect the unknown holistic metabolite of P. carterae. The results show that NMR spectroscopic method is practical in the analysis of metabolites of phytoplankton. The metabolome of P. carterae was dominated by 26 metabolites involved in a number of dif ferent primary and secondary metabolic pathways. Organic acids and their derivatives, amino acids, sugars, nucleic aides were mainly detected. The abundant metabolites are that closely related to the process of cellular osmotic adjustment, which possibly refl ect the active ability of P. carterae to adapt to the versatile coastal niche. DMSP(dimethylsulphoniopropionate) was the most dominant metabolite in P. carterae, up to 2.065±0.278 mg/g lyophilized cells, followed by glutamate and lactose, the contents were 0.349±0.035 and 0.301±0.073 mg/g lyophilized cells respectively. Other metabolites that had the content ranged between 0.1–0.2 mg/g lyophilized cells were alanine, isethionate and arabinose. Amino acid(valine, phenylalanine, isoleucine, tyrosine), organic acid salts(lactate, succinate), scyllitol and uracil had content ranged from 0.01 to below 0.1 mg/g lyophilized cells. Trigonelline, fumarate and formate were detected in very low content(only thousandths of 1 mg per gram of lyophilized cells or below). Our results of the holistic metabolites of P. carterae are the basic references for the further studies when multiple problems will be addressed to this notorious blooming calcifying species.展开更多
基金The National Program on Key Basic Research Project of China under contract Nos 2010CB428900 and 2009CB421202the National Natural Science Foundation of China under contract Nos 41176136,40776093,41276124,41050110436 and 41306118
文摘An investigation was carried out on living coccolithophores （LCs） distribution in the Yellow Sea and the East China Sea from October 17 to November 24, 2011. A total of 223 samples from different depths were col-lected at 48 stations. Totally 18 taxa belonging to coccolithophyceae were identified using a polarized micro-scope at the 1 000× magnification. The maximum species abundance was found at the outside of Transect P. The dominated species wereGephyrocapsa oceanica, Emiliania huxleyi,Helicosphaera carteri,andAlgiros-phaera robusta.The abundance of coccoliths and cells ranged 0-2 965.73 coccoliths/mL, and 0-119.16 cells/mL, with the average values of 471.00 coccoliths/mL and 23.42 cells/mL, respectively. The LCs in surface layer were mainly observed on the coastal belt and middle part of the survey area. The comparison among Transects A, F, P and E indicated lower species diversity and less abundance in the Yellow Sea than those of the East China Sea. The highest abundance of LCs was found in transect F and P. The coccolith abundance increased slightly from surface to bottom in the water column, but the highest value of the cell abundance was observed in the depth of 10-30 m. Temperature, depth and nutrient concentration were suggested as the major environmental factors controlling the distribution and species composition of LCs in the studying area based on canonical correspondence analysis （CCA）.
基金This study was supported by the National Natural Science Foundation of China (Grant No. 39870064) and the State Key Basic Research Development Program (Grant No. G19990433).
文摘Analyses of the original and pyrolytic products of coccolithophore at various temperatures suggest that the contribution of coccolithophore to the formation of large immature oil reservoirs should attract keen interest. Through biochemical processes algae can change inorganic sulphur into organic sulphur, which could be one of the most important precursors of organic sulphur compounds in oil and source rocks. When Methylphenanthrene Index (MPI) and Methylphenanthrene Ratio (MPR) indices were used to evaluate the evolution degree of source rocks and oil maturity, other maturity indices must be used together for correction. In low maturity, the relative abundances of diben-zothiophene (DBT), fluorine (F) and dibenzofuran (DBF) can be used to identify the oxidation-reduction environments.
文摘以北部湾1999年7月至2009年6月11年的Sea Wi FS卫星遥感颗粒无机碳度资料为基础,研究北部湾颗粒无机碳浓度的时空分布特征,并初步探讨其可能的海洋动力调控机制。结果表明:在季节变化上,整个冬季北部湾颗粒无机碳浓度普遍较高,表现出季节平均最大值(0.012 mol·m-3),春季普遍较低(<0.004 mol·m-3),表现出全年最低水平;不同区域的最高水平出现的季节却有所不同:北部湾北部沿岸区域颗粒无机碳浓度在秋季较高,海南岛西部大部分海域在冬季较高,北部湾西北部沿岸则在夏季较高;在空间分布上,近岸海区的颗粒无机碳浓度明显高于海湾中央区域,北部湾北部海域普遍高于南部海域,东部海域高于西部海域。综合相关分析表明,北部湾颗粒无机碳浓度的这一时空分布特征主要同季风的强弱及风向有关,同时也可能与海表温度、流场(如沿岸上升流等)及陆地径流量等有关。
文摘Coccolith production is an important part of the biogenic carbon cycle as the largest source of Calcium carbonate on earth, accounting for about 75% of the deposition of carbon on the sea floor. Recent studies based on laboratory experiment results indicated that increasing anthropogenic CO2 in the atmosphere triggered global ocean acidification leading to a decrease of calcite or aragonite saturation and calcium carbonate, and to decreasing efficiency of carbon export/pumping to deep layers. In the present study, we analyzed about 20 years of field observations of coccolithophore pigment, dissolved inorganic carbon （DIC）, nutrients, and temperatures from the Bermuda Atlantic Time-series Study （BATS） site and satellite remote sensing to investigate the variable tendency of the coccolithophore pigment, and to evaluate the influence of ocean acidification on coccolithophore biomass. The results indicated that there was a generally increasing tendency of coccolithophore pigment, coupled with increasing bicarbonate concentrations or decreasing carbonate ion concentration. The change of coccolithophore pigment was also closely associated with pH, nutrients, mixed layer depth （MLD）, and temperature. Correlation analyses between coceolithophores and abiotic parameter imply that coccoliths production or coccolitho- phore pigment has increased with increasing acidification in the recent 20 years.
基金Foundation item:The National Science and Technology Major Project of the Ministry of Science and Technology of China under contract No.2016ZX05026007-03the National Natural Science Foundation of China under contract Nos 41876046 and 41930536。
文摘Rapid changes on nutrient supply and CO2 concentration that occurred in the northern South China Sea(SCS)during the Early Oligocene,provides an ideal natural laboratory,allowing us to peer into the coccolithophores’physiology in the geological records.In this study,we established a new nannofossil assemblage index,termed as E^*ratio,which is calculated by the relative abundance of eutrophic taxa and meso-oligotrophic taxa(E^*=e/e+c,where e is eutrophic taxa,and c is meso-oligotrophic taxa)Eutrophic taxa include small Reticulofenestra,Reticulofenestra lockeri group,Reticulofenestra bisecta group and Coccolithus pelagicus group,while meso-oligotrophic taxa include Cyclicargolithus spp.The E^*ratio is well correlated with nutrient proxy during the Early Oligocene,while with different covarying patterns under the higher and lower CO2 condition.By comparing the assemblage changes to the published data,we suggest that coccolithophores may change the way they use carbon source and nutrient with the decline of CO2.Furthermore,this implies a possible initiation of the carbon concentrating mechanism.
基金Supported by the Natural Science Foundation of Zhengjiang Province(No.LY12D06001)the Academic Discipline Project of Ningbo University(No.XKl15D236)+4 种基金the Zhejiang Marine Biotechnology Innovation Team(ZMBIT)(No.2010R50029)the National Natural Science Foundation of China(No.31172448)the Project of Ministry of Education,China(No.20133305130001)the Zhejiang Natural Science Foundation(No.LY15C190004)the K.C.Wong Magna Fund of Ningbo University
文摘Pleurochrysis carterae is a calcifi ed coccolithophorid species that usually blooms in the coastal area and causes aquaculture losses. The cellular calcifi cation, blooming and many other critical species specifi c eco-physiological processes are closely related to various metabolic pathways. The purpose of this study is to apply the unbiased and non-destructive method of nuclear magnetic resonance(NMR) to detect the unknown holistic metabolite of P. carterae. The results show that NMR spectroscopic method is practical in the analysis of metabolites of phytoplankton. The metabolome of P. carterae was dominated by 26 metabolites involved in a number of dif ferent primary and secondary metabolic pathways. Organic acids and their derivatives, amino acids, sugars, nucleic aides were mainly detected. The abundant metabolites are that closely related to the process of cellular osmotic adjustment, which possibly refl ect the active ability of P. carterae to adapt to the versatile coastal niche. DMSP(dimethylsulphoniopropionate) was the most dominant metabolite in P. carterae, up to 2.065±0.278 mg/g lyophilized cells, followed by glutamate and lactose, the contents were 0.349±0.035 and 0.301±0.073 mg/g lyophilized cells respectively. Other metabolites that had the content ranged between 0.1–0.2 mg/g lyophilized cells were alanine, isethionate and arabinose. Amino acid(valine, phenylalanine, isoleucine, tyrosine), organic acid salts(lactate, succinate), scyllitol and uracil had content ranged from 0.01 to below 0.1 mg/g lyophilized cells. Trigonelline, fumarate and formate were detected in very low content(only thousandths of 1 mg per gram of lyophilized cells or below). Our results of the holistic metabolites of P. carterae are the basic references for the further studies when multiple problems will be addressed to this notorious blooming calcifying species.
