A new triterpenoid,possessing a lanost-9（11）-3-one skeleton,was isolated from the roots of Kadsura coccinea,based on the spectral analysis,its structure was elucidated as 3-hydroxy-12-acetoxycoccinic acid.
AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. The...AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS. RESULTS: Two compounds were determined as(7′S,8′S,8R)-(8β,8′α)- dimethyl-4,4′-dihydroxy-5,3′-dimethoxy-5′-cyclolignan glucoside(1) and micrandiactone H(2), respectively. CONCLUSION: Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide(NO) production in lipopolysaccharide-induced RAW264.7 macrophages.展开更多
Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of ani...Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of animals were divided each containing six animals.Two wound models including incision and excision wound models were used in this study.The parameters studied were tensile strength on incision wound model and in terms of wound contraction for excision wound model were compared with standard Nitrofurazone(NFZ) ointment(0.2%w/w). Six extracts(ethanol.aqueous,petroleum ether,benzene,chloroform and ethyl acetate ) of I. coccinea were screened for in vitro growth inhibiting activity against different bacterial strains riz. Staphylococcia aureus.Bacillus pumilius,Enterococcus facialis.Escherichia coli,Salmonella typhi and Pseudomonas aeruginosa and fungi Candida albicans and Aspergillus niger were compared with the standard drugs ciprofloxacin and chloramphenicol for antibacterial and griscofulvin for antifungal screening.The serial dilution and cup(or) well plate methods were used for the antimicrobial study and MIC was determined.Results:The ethanolic extract showed significant (P【0.001) wound healing activity when compared to standard drug NFZ with respect to normal control group.Amongst all.ethanolic extract showed highly significant antibacterial activity against all bacterial strains used in this study when compared to standard.The aqueous extract showed moderate significant inhibition against all bacterial strains when compared to standard. All the extracts were shown negligible activity against the fungal strains used in this study. Conclusions:The ethanolic root extract of I.coccinea showed pronounced wound healing and antibacterial activity.The probable reason to heal the wound was that the external application of the extract prevented the microbes to invade through the wound thus the protection of wound occurs against the infection of the various organisms.展开更多
Background: Few studies have often focused on medicinal plant mixtures, yet the most used in low-and middle-income areas as alternative drug to treat diseases. Objective: To evaluate the antidiabetic effects of Emilia...Background: Few studies have often focused on medicinal plant mixtures, yet the most used in low-and middle-income areas as alternative drug to treat diseases. Objective: To evaluate the antidiabetic effects of Emilia coccinea (Ec) (Asteraceae), Scoparia dulcis (Sd) (Plantaginaceae) and Tetracarpidium conophorum (Tc) (Euphorbiaceae) aqueous extracts mixture (EcSdTc) in rats. Methodology: Single plant aqueous extracts (Ec, Sd and Tc) and their mixtures (EcSd, EcTc, SdTc and EcSdTc) (each at the doses of 125 and 250 mg/kg body weight respectively) were evaluated in acute administration on blood glucose in normal, glucose-overloaded and diabetic rats;then EcSdTc mixture was assessed in prolonged administration (21 days) on blood glucose, body weight, serum biochemical and antioxidant parameters in diabetic rats. Diabetes was induced by single intraperitoneal administration of streptozotocin (STZ;50 mg/kg), and glibenclamide (10 mg/kg) was used as standard drug. Results: In acute administration, EcTc250, EcSdTc125, SdTc250, SdTc125, EcSd250, and EcSdTc250 extracts mixtures reduced (p Conclusion: EcSdTc aqueous extracts mixture has potent hypoglycemic and antidiabetic effects, probably due to their bioactive compounds synergistic and/or additive actions, justifying its traditional use as alternative remedies.展开更多
Ixora coccinea Linn., （Rubiaceae） commonly known as jungle of geranium and red ixora, is an evergreen shrub found throughout India. Depending on the medical condition, the flowers, leaves, roots, and the stem are us...Ixora coccinea Linn., （Rubiaceae） commonly known as jungle of geranium and red ixora, is an evergreen shrub found throughout India. Depending on the medical condition, the flowers, leaves, roots, and the stem are used to treat various ailments in the Indian traditional system of medicine, the Ayurveda, and also in various folk medicines. The fruits, when fully ripe, are used as a dietary source. Phytochemical studies indicate that the plant contains important phytochemicals such as lupeol, ursolic acid, oleanolic acid, sitosterol, rutin, lecocyanadin, anthocyanins, proanthocyanidins, glycosides of kaempferol and quercetin. Pharmacological studies suggest that the plant possesses antioxidative, antibacterial, gastroprotective, hepatoprotective, antidiarrhoeal, antinociceptive, antimutagenic, antineoplastic and chemopreventive effects, thus lending scientific support to the plant＇s ethnomedicinal uses. In the present review, efforts are made in addressing its ethnomedicinal uses, chemical constituents, and validated pharmacological observations.展开更多
Four new rearranged 6/6/5/6-fused lanostane-type triterpenoids,kadcoccitanes A-D(1-4),were isolated from the roots of Kadsura coccinea,and their structures were mainly elucidated by comprehensive analysis of their spe...Four new rearranged 6/6/5/6-fused lanostane-type triterpenoids,kadcoccitanes A-D(1-4),were isolated from the roots of Kadsura coccinea,and their structures were mainly elucidated by comprehensive analysis of their spectroscopic data.Additionally,the structure of 1 was ambiguously verifed by single-crystal X-ray difraction,while the structure of 2,which features a novel 8,16-epoxy motif,was validated by quantum chemical calculation of NMR parameters and ECD spectrum.Moreover,1 and 4 were found to exhibited anticoagulant activity,while 3 and 4 were found to possess anti-platelet aggregation activity.展开更多
The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chrom...The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.展开更多
[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rat...[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.展开更多
Four new 14(13→12)-abeolanostane triterpenoids featuring extendedπ-conjugated systems,kadcoccitanes E-H(1-4),were obtained from the stems of Kadsura coccinea through using a HPLC−UV-guided approach.Their structural ...Four new 14(13→12)-abeolanostane triterpenoids featuring extendedπ-conjugated systems,kadcoccitanes E-H(1-4),were obtained from the stems of Kadsura coccinea through using a HPLC−UV-guided approach.Their structural and configurational determination was accomplished through extensive spectroscopic analysis coupled with quantum chemical calculations.Kadcoccitanes E-H were tested for their cytotoxic activities against five human tumor cell lines(HL-60,A-549,SMMC-7721,MDA-MB-231,SW-480)but none of them exhibited activities at the concentration 40μM.展开更多
Green synthesis of metal oxide nanoparticles using plant extract is a promising alternative to traditional method of chemical synthesis. In this paper, we report the synthesis of nanostructured zinc oxide particles by...Green synthesis of metal oxide nanoparticles using plant extract is a promising alternative to traditional method of chemical synthesis. In this paper, we report the synthesis of nanostructured zinc oxide particles by biological method. Highly stable and spherical zinc oxide nanoparticles are produced by using zinc acetate and Ixora coccinea leaf extract. Formation of zinc oxide nanoparticles has been confirmed by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering analysis (DLS), zetapotential study and Scanning Electron Microscope with the Energy Dispersive X-ray studies (EDX). Dynamic light scattering analysis shows average particle size of 145.1 nm whereas high zeta potential value confirms the stability of formed zinc oxide nanoparticles. The Scanning Electron Microscope reveals spherical morphology of nanoparticles and Energy Dispersive X-ray analysis confirms the formation of highly pure zinc oxide nanoparticles. The zinc oxide nanoparticles from Ixora coccinea leaves are expected to have applications in biomedical, cosmetic industries, biotechnology, sensors, medical, catalysis, optical device, coatings, drug delivery and water remediation, and also may be applied for electronic and magneto-electric devices. This new eco-friendly approach of synthesis is a novel, cheap, and convenient technique suitable for large scale commercial production.展开更多
Musa beccarii N.W.Simmonds var. beccarii and Musa beccarii N.W.Simmonds var. hottana H?kkinen were described earlier in Acta Phytotaxonomica et Geobotanica based on their morphological characteristics. In order to dis...Musa beccarii N.W.Simmonds var. beccarii and Musa beccarii N.W.Simmonds var. hottana H?kkinen were described earlier in Acta Phytotaxonomica et Geobotanica based on their morphological characteristics. In order to distinguish the genomes between the two M. beccarii varieties and M. coccinea Andrews, we have now studied them with the Inter- Retrotransposon Amplified Polymorphism (IRAP) marker analyses. The high levels of IRAP polymorphism detected in this study showed that M. beccarii var. beccarii and M. beccarii var. hottana are two distinct varieties. Additional IRAP bands found in M. beccarii var. beccarii indicated that M. beccarii var. hottana might be evolutionarily more ancient than M. beccarii var. beccarii because LTR retrotransposons are not transpositionally removed.展开更多
Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 in...Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats and associated complications.Methodology: In vitro radical scavenging activity of I. coccinea was assessed using DPPH, FRAP and hydrogen peroxide. In vivo antiobesity and antihyperlipidimic activity of I. coccinea was tested in Triton X-100 induced hyperlipidemic rats and assessed for its biochemical parameters in blood and tissue samples.The relationship between physiological responses and regulation of body temperature was investigated by using animal surface temperature images captured with infrared camera.Results: The results of mineral analysis, antioxidant, total flavonoid and phenolic content represented high amount of mineral and had the potential to scavenge free radicals tested with DPPH, FRAP and hydrogen peroxide radicals with dose dependent activity. The highest activity was observed in aqueous extract, DPPH with 71.5% inhibition, FRAP with 56.8%, H2 O2 with 33% activity at 100 μg/mL concentration.Triton X-100 induced hyperlipidemic rats when treated with I. coccinea aqueous extract showed significant activity by regulating the biochemical parameters and maintaining the lipid profile by decreasing TC,LDL-C, VLDL-C, TG and improving HDL-C levels. Similarly, the elevated levels of creatinine, urea, uric acid,AST, ALT, ALP due to induction of hyperlipidemia, were brought back to near normal levels after treatment with I. coccinea. The levels of tissue anti-oxidants enzymes like SOD and CAT were also found to be improved in treated I. coccinea groups. The whole body asymmetrical temperature distribution analysis showed that significant decreases in temperature was observed in obesity induced groups but a gradual increase in temperature(2%–5%) was observed after treatment.Conclusion: Thus, the results indicated that I. coccinea can be a drug of choice to decrea展开更多
Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and...Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at R<sub>f</sub>0.55.展开更多
Kadsura coccinea(Lem.)is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds.The non-editable part of the fruit,i.e.,the seed and peel,represents more than 60%of the fruit and is ...Kadsura coccinea(Lem.)is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds.The non-editable part of the fruit,i.e.,the seed and peel,represents more than 60%of the fruit and is considered a biowaste.This significantly restricts the development of the K.coccinea fruit industry.Clarifying the metabolic components of the different fruit parts can help to improve the utilization rate and valorization of K.coccinea.Herein,we evaluated K.coccinea fruit peel,pulp,and seed using widely-targeted metabolomics and quantified a set of 736 bioactive compounds from 11 major metabolite classes.The most prominent metabolite classes included lipids,amino acids,flavonoids,and lignans.Furthermore,our results emphasized a significant accumulation of flavonoids in pulp tissues,while alkaloids and lignans were abundant in peel and seed tissues,respectively.A total of 183 metabolites were differentially accumulated among the three tissues.Procyanidin C2,rutinoside,2-hydroxyoleanolic acid,5-hydroxymethyluracil,nootkatol,isoquercitrin,isohyperoside,quercetin-7-O-glucoside,hyperin,and rutin showed elevated accumulation in the peel.In the seed,kadsuralignan G,kadcoccilactone A,kadsuralignan H,lysoPE 20:5,iso-schisandrin ethyl alcohol,and kadangustin were significantly enriched.Our results highlight the diverse metabolome composition of K.coccinea fruit parts,which can be further exploited for its valorization in various industries.展开更多
[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materia...[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materials in order to investigate and analyze leaf SPAD value, relative water content （RWC）, malonaldehyde （MAD）, cell membrane permeability （RP）, proline （PRO） and superoxide dismutase activity （SOD）. [Result] As drought stress improved, leaf SPAD value and RWC were both diminishing and RWC fell remarkably; MAD, Pro, RP and SOD activity were increasing; MAD and RP grew significantly. [Conclusion] Kadsura coccinea is much sensitive to drought stress and will be affected if water loss is too much high.展开更多
Terpenoids are the largest class of natural products with complex structures and extensive bioactivities;their scaffolds are generated by diverse terpenoid synthases(TPSs)from a limited number of isoprenoid diphosphat...Terpenoids are the largest class of natural products with complex structures and extensive bioactivities;their scaffolds are generated by diverse terpenoid synthases(TPSs)from a limited number of isoprenoid diphosphate precursors.Promiscuous TPSs play important roles in the evolution of terpenoid chemodiversity,but they remain largely unappreciated.Here,an extremely promiscuous terpenoid synthase(CcTPS1)of the TPS-b subfamily was cloned and functionally characterized from a leaf-specific transcriptome of the Lamiaceae plant Colquhounia coccinea var.mollis.CcTPS1 is the first sester-/di-/sesqui-/mono-TPS identified from the plant kingdom,accepting C_(25)/C_(20)/C_(15)/C_(10) diphosphate substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids.Engineered Escherichia coli expressing CcTPS1 produced three previously unreported terpenoids(two sesterterpenoids and a diterpenoid)with rare cyclohexanecontaining skeletons,along with four sesquiterpenoids and one monoterpenoid.Their structures were elucidated by extensive nuclear magnetic resonance spectroscopy.Nicotiana benthamiana transiently expressing CcTPS1 also produced the diterpenoid and sesquiterpenoids,demonstrating the enzyme’s promiscuity in planta.Its highly leaf-specific expression pattern combined with detectable terpenoid products in leaves of C.coccinea var.mollis and N.benthamiana expressing CcTPS1 suggested that CcTPS1 was mainly responsible for diterpenoid and sesquiterpenoid biosynthesis in plants.CcTPS1 expression and the terpenoid products could be induced by methyl jasmonate,suggesting their possible role in plant–environment interaction.CcTPS1 was localized to the cytosol and may differ from mono-TPSs in subcellular compartmentalization and substrate tolerance.These findings will greatly aid our understanding of plant TPS evolution and terpenoid chemodiversity;they also highlight the enormous potential of transcriptome mining and heterologous expression for the exploration of unique enzymes and natural products hidden in plants.展开更多
文摘A new triterpenoid,possessing a lanost-9（11）-3-one skeleton,was isolated from the roots of Kadsura coccinea,based on the spectral analysis,its structure was elucidated as 3-hydroxy-12-acetoxycoccinic acid.
基金supported by the Program for New Century Excellent Talents in University(NCET-12-0977)the National New Drug Innovation Great Project of China(2011ZX09307-002-02)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in a University(PCSIRT-IRT1193)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS. RESULTS: Two compounds were determined as(7′S,8′S,8R)-(8β,8′α)- dimethyl-4,4′-dihydroxy-5,3′-dimethoxy-5′-cyclolignan glucoside(1) and micrandiactone H(2), respectively. CONCLUSION: Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide(NO) production in lipopolysaccharide-induced RAW264.7 macrophages.
文摘Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of animals were divided each containing six animals.Two wound models including incision and excision wound models were used in this study.The parameters studied were tensile strength on incision wound model and in terms of wound contraction for excision wound model were compared with standard Nitrofurazone(NFZ) ointment(0.2%w/w). Six extracts(ethanol.aqueous,petroleum ether,benzene,chloroform and ethyl acetate ) of I. coccinea were screened for in vitro growth inhibiting activity against different bacterial strains riz. Staphylococcia aureus.Bacillus pumilius,Enterococcus facialis.Escherichia coli,Salmonella typhi and Pseudomonas aeruginosa and fungi Candida albicans and Aspergillus niger were compared with the standard drugs ciprofloxacin and chloramphenicol for antibacterial and griscofulvin for antifungal screening.The serial dilution and cup(or) well plate methods were used for the antimicrobial study and MIC was determined.Results:The ethanolic extract showed significant (P【0.001) wound healing activity when compared to standard drug NFZ with respect to normal control group.Amongst all.ethanolic extract showed highly significant antibacterial activity against all bacterial strains used in this study when compared to standard.The aqueous extract showed moderate significant inhibition against all bacterial strains when compared to standard. All the extracts were shown negligible activity against the fungal strains used in this study. Conclusions:The ethanolic root extract of I.coccinea showed pronounced wound healing and antibacterial activity.The probable reason to heal the wound was that the external application of the extract prevented the microbes to invade through the wound thus the protection of wound occurs against the infection of the various organisms.
文摘Background: Few studies have often focused on medicinal plant mixtures, yet the most used in low-and middle-income areas as alternative drug to treat diseases. Objective: To evaluate the antidiabetic effects of Emilia coccinea (Ec) (Asteraceae), Scoparia dulcis (Sd) (Plantaginaceae) and Tetracarpidium conophorum (Tc) (Euphorbiaceae) aqueous extracts mixture (EcSdTc) in rats. Methodology: Single plant aqueous extracts (Ec, Sd and Tc) and their mixtures (EcSd, EcTc, SdTc and EcSdTc) (each at the doses of 125 and 250 mg/kg body weight respectively) were evaluated in acute administration on blood glucose in normal, glucose-overloaded and diabetic rats;then EcSdTc mixture was assessed in prolonged administration (21 days) on blood glucose, body weight, serum biochemical and antioxidant parameters in diabetic rats. Diabetes was induced by single intraperitoneal administration of streptozotocin (STZ;50 mg/kg), and glibenclamide (10 mg/kg) was used as standard drug. Results: In acute administration, EcTc250, EcSdTc125, SdTc250, SdTc125, EcSd250, and EcSdTc250 extracts mixtures reduced (p Conclusion: EcSdTc aqueous extracts mixture has potent hypoglycemic and antidiabetic effects, probably due to their bioactive compounds synergistic and/or additive actions, justifying its traditional use as alternative remedies.
文摘Ixora coccinea Linn., （Rubiaceae） commonly known as jungle of geranium and red ixora, is an evergreen shrub found throughout India. Depending on the medical condition, the flowers, leaves, roots, and the stem are used to treat various ailments in the Indian traditional system of medicine, the Ayurveda, and also in various folk medicines. The fruits, when fully ripe, are used as a dietary source. Phytochemical studies indicate that the plant contains important phytochemicals such as lupeol, ursolic acid, oleanolic acid, sitosterol, rutin, lecocyanadin, anthocyanins, proanthocyanidins, glycosides of kaempferol and quercetin. Pharmacological studies suggest that the plant possesses antioxidative, antibacterial, gastroprotective, hepatoprotective, antidiarrhoeal, antinociceptive, antimutagenic, antineoplastic and chemopreventive effects, thus lending scientific support to the plant＇s ethnomedicinal uses. In the present review, efforts are made in addressing its ethnomedicinal uses, chemical constituents, and validated pharmacological observations.
基金supported financially by the National Natural Science Foundation of China(Nos.81874298 and 81673329).
文摘Four new rearranged 6/6/5/6-fused lanostane-type triterpenoids,kadcoccitanes A-D(1-4),were isolated from the roots of Kadsura coccinea,and their structures were mainly elucidated by comprehensive analysis of their spectroscopic data.Additionally,the structure of 1 was ambiguously verifed by single-crystal X-ray difraction,while the structure of 2,which features a novel 8,16-epoxy motif,was validated by quantum chemical calculation of NMR parameters and ECD spectrum.Moreover,1 and 4 were found to exhibited anticoagulant activity,while 3 and 4 were found to possess anti-platelet aggregation activity.
基金supported by the PAPD(Priority Academic Program Development)of Jiangsu Higher Education Institutions,University Science Research Project of Jiangsu Province(No.09KJB350002)National Key Technology R&D Program(No.2012BAI30B02),National Natural Science Foundation of China(No.30901853)Hepatic Fibrosis Innovative Medicine-Research and Development of Fructus Schisandrae capsule(No.F12-157-9-00)
文摘The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.
基金Supported by National Natural Science Foundation of China(No.81303162)China Postdoctoral Science Foundation(No.2015T80784,2014M552017)Postdoctoral Scientific Research Project of Henan Province(No.2013010)
文摘[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.
基金Natural Science Foundation of Yunnan Province(202101AT070188).
文摘Four new 14(13→12)-abeolanostane triterpenoids featuring extendedπ-conjugated systems,kadcoccitanes E-H(1-4),were obtained from the stems of Kadsura coccinea through using a HPLC−UV-guided approach.Their structural and configurational determination was accomplished through extensive spectroscopic analysis coupled with quantum chemical calculations.Kadcoccitanes E-H were tested for their cytotoxic activities against five human tumor cell lines(HL-60,A-549,SMMC-7721,MDA-MB-231,SW-480)but none of them exhibited activities at the concentration 40μM.
文摘Green synthesis of metal oxide nanoparticles using plant extract is a promising alternative to traditional method of chemical synthesis. In this paper, we report the synthesis of nanostructured zinc oxide particles by biological method. Highly stable and spherical zinc oxide nanoparticles are produced by using zinc acetate and Ixora coccinea leaf extract. Formation of zinc oxide nanoparticles has been confirmed by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering analysis (DLS), zetapotential study and Scanning Electron Microscope with the Energy Dispersive X-ray studies (EDX). Dynamic light scattering analysis shows average particle size of 145.1 nm whereas high zeta potential value confirms the stability of formed zinc oxide nanoparticles. The Scanning Electron Microscope reveals spherical morphology of nanoparticles and Energy Dispersive X-ray analysis confirms the formation of highly pure zinc oxide nanoparticles. The zinc oxide nanoparticles from Ixora coccinea leaves are expected to have applications in biomedical, cosmetic industries, biotechnology, sensors, medical, catalysis, optical device, coatings, drug delivery and water remediation, and also may be applied for electronic and magneto-electric devices. This new eco-friendly approach of synthesis is a novel, cheap, and convenient technique suitable for large scale commercial production.
文摘Musa beccarii N.W.Simmonds var. beccarii and Musa beccarii N.W.Simmonds var. hottana H?kkinen were described earlier in Acta Phytotaxonomica et Geobotanica based on their morphological characteristics. In order to distinguish the genomes between the two M. beccarii varieties and M. coccinea Andrews, we have now studied them with the Inter- Retrotransposon Amplified Polymorphism (IRAP) marker analyses. The high levels of IRAP polymorphism detected in this study showed that M. beccarii var. beccarii and M. beccarii var. hottana are two distinct varieties. Additional IRAP bands found in M. beccarii var. beccarii indicated that M. beccarii var. hottana might be evolutionarily more ancient than M. beccarii var. beccarii because LTR retrotransposons are not transpositionally removed.
文摘Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats and associated complications.Methodology: In vitro radical scavenging activity of I. coccinea was assessed using DPPH, FRAP and hydrogen peroxide. In vivo antiobesity and antihyperlipidimic activity of I. coccinea was tested in Triton X-100 induced hyperlipidemic rats and assessed for its biochemical parameters in blood and tissue samples.The relationship between physiological responses and regulation of body temperature was investigated by using animal surface temperature images captured with infrared camera.Results: The results of mineral analysis, antioxidant, total flavonoid and phenolic content represented high amount of mineral and had the potential to scavenge free radicals tested with DPPH, FRAP and hydrogen peroxide radicals with dose dependent activity. The highest activity was observed in aqueous extract, DPPH with 71.5% inhibition, FRAP with 56.8%, H2 O2 with 33% activity at 100 μg/mL concentration.Triton X-100 induced hyperlipidemic rats when treated with I. coccinea aqueous extract showed significant activity by regulating the biochemical parameters and maintaining the lipid profile by decreasing TC,LDL-C, VLDL-C, TG and improving HDL-C levels. Similarly, the elevated levels of creatinine, urea, uric acid,AST, ALT, ALP due to induction of hyperlipidemia, were brought back to near normal levels after treatment with I. coccinea. The levels of tissue anti-oxidants enzymes like SOD and CAT were also found to be improved in treated I. coccinea groups. The whole body asymmetrical temperature distribution analysis showed that significant decreases in temperature was observed in obesity induced groups but a gradual increase in temperature(2%–5%) was observed after treatment.Conclusion: Thus, the results indicated that I. coccinea can be a drug of choice to decrea
文摘Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at R<sub>f</sub>0.55.
文摘Kadsura coccinea(Lem.)is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds.The non-editable part of the fruit,i.e.,the seed and peel,represents more than 60%of the fruit and is considered a biowaste.This significantly restricts the development of the K.coccinea fruit industry.Clarifying the metabolic components of the different fruit parts can help to improve the utilization rate and valorization of K.coccinea.Herein,we evaluated K.coccinea fruit peel,pulp,and seed using widely-targeted metabolomics and quantified a set of 736 bioactive compounds from 11 major metabolite classes.The most prominent metabolite classes included lipids,amino acids,flavonoids,and lignans.Furthermore,our results emphasized a significant accumulation of flavonoids in pulp tissues,while alkaloids and lignans were abundant in peel and seed tissues,respectively.A total of 183 metabolites were differentially accumulated among the three tissues.Procyanidin C2,rutinoside,2-hydroxyoleanolic acid,5-hydroxymethyluracil,nootkatol,isoquercitrin,isohyperoside,quercetin-7-O-glucoside,hyperin,and rutin showed elevated accumulation in the peel.In the seed,kadsuralignan G,kadcoccilactone A,kadsuralignan H,lysoPE 20:5,iso-schisandrin ethyl alcohol,and kadangustin were significantly enriched.Our results highlight the diverse metabolome composition of K.coccinea fruit parts,which can be further exploited for its valorization in various industries.
基金Supported by Guangxi Science Research and Technology Development Planning Program(11107010-1-411107010-1-4-1)~~
文摘[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materials in order to investigate and analyze leaf SPAD value, relative water content （RWC）, malonaldehyde （MAD）, cell membrane permeability （RP）, proline （PRO） and superoxide dismutase activity （SOD）. [Result] As drought stress improved, leaf SPAD value and RWC were both diminishing and RWC fell remarkably; MAD, Pro, RP and SOD activity were increasing; MAD and RP grew significantly. [Conclusion] Kadsura coccinea is much sensitive to drought stress and will be affected if water loss is too much high.
基金supported financially by the National Natural Science Foundation of China(21937006,31770390,31770340 and 31525005)the Yunnan Key Research and Development Program(no.2019ZF011-2)+1 种基金the Science Foundation of Yunnan(2018FA017)the Youth Innovation Promotion association and“Western Light”Program of the CAS(to Y.Liu).
文摘Terpenoids are the largest class of natural products with complex structures and extensive bioactivities;their scaffolds are generated by diverse terpenoid synthases(TPSs)from a limited number of isoprenoid diphosphate precursors.Promiscuous TPSs play important roles in the evolution of terpenoid chemodiversity,but they remain largely unappreciated.Here,an extremely promiscuous terpenoid synthase(CcTPS1)of the TPS-b subfamily was cloned and functionally characterized from a leaf-specific transcriptome of the Lamiaceae plant Colquhounia coccinea var.mollis.CcTPS1 is the first sester-/di-/sesqui-/mono-TPS identified from the plant kingdom,accepting C_(25)/C_(20)/C_(15)/C_(10) diphosphate substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids.Engineered Escherichia coli expressing CcTPS1 produced three previously unreported terpenoids(two sesterterpenoids and a diterpenoid)with rare cyclohexanecontaining skeletons,along with four sesquiterpenoids and one monoterpenoid.Their structures were elucidated by extensive nuclear magnetic resonance spectroscopy.Nicotiana benthamiana transiently expressing CcTPS1 also produced the diterpenoid and sesquiterpenoids,demonstrating the enzyme’s promiscuity in planta.Its highly leaf-specific expression pattern combined with detectable terpenoid products in leaves of C.coccinea var.mollis and N.benthamiana expressing CcTPS1 suggested that CcTPS1 was mainly responsible for diterpenoid and sesquiterpenoid biosynthesis in plants.CcTPS1 expression and the terpenoid products could be induced by methyl jasmonate,suggesting their possible role in plant–environment interaction.CcTPS1 was localized to the cytosol and may differ from mono-TPSs in subcellular compartmentalization and substrate tolerance.These findings will greatly aid our understanding of plant TPS evolution and terpenoid chemodiversity;they also highlight the enormous potential of transcriptome mining and heterologous expression for the exploration of unique enzymes and natural products hidden in plants.
