目的：根据WHO（2003版）乳腺肿瘤分类标准，探讨军事医学科学院附属医院乳腺肿瘤的病理类型及分布特点。方法：收集2009年8月~2014年8月军事医学科学院附属医院诊治2392例乳腺肿瘤，复习其临床资料、HE及免疫组织化学切片。按WHO（200...目的：根据WHO（2003版）乳腺肿瘤分类标准，探讨军事医学科学院附属医院乳腺肿瘤的病理类型及分布特点。方法：收集2009年8月~2014年8月军事医学科学院附属医院诊治2392例乳腺肿瘤，复习其临床资料、HE及免疫组织化学切片。按WHO（2003版）分类标准进行病理诊断及分类。结果：2392例乳腺肿瘤中，绝大多数为女性患者（2382例），占99.58%，男性10例（0.42%）。乳腺良性肿瘤982例（41.05%），浸润性乳腺癌和原位癌1389例（58.07%），乳腺恶性和交界性叶状肿瘤10例（0.42%），淋巴瘤2例（0.08%），转移性肿瘤9例（0.38%）。其中乳腺良性肿瘤中构成比居前5位分别为纤维腺瘤、导管内乳头状瘤、叶状肿瘤、脂肪瘤和管状腺瘤；纤维腺瘤患者发病高峰为20~40岁。浸润性乳腺癌中浸润性导管癌1112例（87.63%），浸润性小叶癌37例（2.91%），其他特殊类型癌120例（9.46%）。乳腺癌中以浸润性导管癌、浸润性小叶癌、基底细胞样乳腺癌、黏液癌最为多见。浸润性导管癌发病年龄以40~60岁居多。导管原位癌61例，导管内癌伴微浸润35例，小叶原位癌3例。Paget病10例（其中4例合并浸润性癌，4例合并导管内癌且3例伴微浸润）。乳腺癌中分子亚型分类Luminal A型693例（60.63%）；Luminal B型127例（11.11%）；Her-2过表达型130例（11.37%）；正常乳腺样（normal breast -like）型172例（15.05%）；Basal-like型21例（1.84%）。结论：对乳腺定期检查，提高早期诊断率，以期早期发现及时治疗。展开更多
Objective： The novel fully automated immunohistochemistry （IHC） assay-Ventana anaplastic lymphoma kinase （ALK）-DSF3 for screening ALK rearrangements has been approved by China＇s Food and Drug Administration in 2...Objective： The novel fully automated immunohistochemistry （IHC） assay-Ventana anaplastic lymphoma kinase （ALK）-DSF3 for screening ALK rearrangements has been approved by China＇s Food and Drug Administration in 2013, our previous study disclosed a highly specificity and sensitivity nearly 100%, and its efficacy needs to be evaluated in a large cohort of primary lung adenocarcinoma patients, and to compare clinicopathological features with ALK （＋） and ALK （-） lung adenocarcinoma.Methods： A total of 1,504 consecutive surgical lung adenocareinoma eases of Chinese Han population were collected and re-diagnosed according to the 2011 multidisciplinary classification of lung adenocarcinoma. Fully automated Ventana ALK-D5F3 IHC staining with a binary scoring was adopted to evaluate staining and correlated with dinieopathologieal characters, including age, sex, differentiation degree, histological subtype, lymph node metastasis, and clinical staging. ALK （＋） patients were followed-up, and targeted therapy of ALKinhibitors was adopted and observed in patients with stage IV according to the NCCN guideline.Results： ALK positive adenocarcinomas were identified in 6.6% of the surgically resected 1,504 NSCLCs, and significantly younger than the negative group （P〈0.05）.Mucinous adenocarcinoma （28.2%） was determined to be predominant in ALK （＋） cases, followed by the solid type （11.7%）, specific type （6.8%）, papillary type （5.6%）, acinar type （5.5%）, and lepidic type （3.1%）, and the differences were statistically significant （χ2=42.01 1, P〈0.05）. ALK （＋） adenocarcinoma with lymph node metastasis （10.8%） were significantly higher than that without lymph node metastasis （4.5%） （g2=19.809, P〈0.05）; and ALK （＋） in phase Ⅳ （20%） was significantly higher than phaseⅢ （12.9%）, phase Ⅱ （4.2%）, phase Ⅰ （4.5%）, and phase 0 （0） （g2=36.068, P〈0.05）. Multivariate logistic regression disclosed that patient age, A展开更多
目的:分析食管原发性恶性黑色素瘤(primary malignant melanoma of the esophagus,PMME)的病理诊断特点、鉴别诊断、生物学行为及预后。方法:收集安阳市肿瘤医院5例PMME,对其进行光镜观察、免疫组织化学染色、临床病理分析并进行随访。...目的:分析食管原发性恶性黑色素瘤(primary malignant melanoma of the esophagus,PMME)的病理诊断特点、鉴别诊断、生物学行为及预后。方法:收集安阳市肿瘤医院5例PMME,对其进行光镜观察、免疫组织化学染色、临床病理分析并进行随访。结果:PMME多见于中老年人,常发生于食管中段,大体呈蕈伞型。光镜下瘤细胞异型性明显,呈梭形、卵圆形或不规则形,以梭形细胞为主。肿瘤细胞胞核体积大,核仁清晰、染色质丰富。瘤细胞形态多样且无色素易误诊为分化差的鳞状细胞癌、肉瘤样癌、淋巴瘤等。免疫组织化学结果肿瘤细胞显示vimentin,S-100,HMB-45和Melan-A阳性;不表达神经特异性烯醇化酶(neuron-specific enolase,NSE)、白细胞共同抗原(leukocyte common antigen,LCA)、细胞角蛋白(cytokeratin,CK)、嗜铬素蛋白-A(chromogranin proteins A,Cg A)和突触素(syn)。随瘤细胞浸润不同组织层次可伴有淋巴结转移。结论:PMME是一种罕见肿瘤,恶性度高、预后差。由于其症状与食管其它肿瘤相似,在活检或手术前很难确诊,对发生于中老年人的食管中段蕈伞型肿物应考虑原发性恶性黑色素瘤的可能。临床病理分析肿瘤大小、浸润层次、淋巴结转移及治疗方式可能影响预后。展开更多
文摘目的：根据WHO（2003版）乳腺肿瘤分类标准，探讨军事医学科学院附属医院乳腺肿瘤的病理类型及分布特点。方法：收集2009年8月~2014年8月军事医学科学院附属医院诊治2392例乳腺肿瘤，复习其临床资料、HE及免疫组织化学切片。按WHO（2003版）分类标准进行病理诊断及分类。结果：2392例乳腺肿瘤中，绝大多数为女性患者（2382例），占99.58%，男性10例（0.42%）。乳腺良性肿瘤982例（41.05%），浸润性乳腺癌和原位癌1389例（58.07%），乳腺恶性和交界性叶状肿瘤10例（0.42%），淋巴瘤2例（0.08%），转移性肿瘤9例（0.38%）。其中乳腺良性肿瘤中构成比居前5位分别为纤维腺瘤、导管内乳头状瘤、叶状肿瘤、脂肪瘤和管状腺瘤；纤维腺瘤患者发病高峰为20~40岁。浸润性乳腺癌中浸润性导管癌1112例（87.63%），浸润性小叶癌37例（2.91%），其他特殊类型癌120例（9.46%）。乳腺癌中以浸润性导管癌、浸润性小叶癌、基底细胞样乳腺癌、黏液癌最为多见。浸润性导管癌发病年龄以40~60岁居多。导管原位癌61例，导管内癌伴微浸润35例，小叶原位癌3例。Paget病10例（其中4例合并浸润性癌，4例合并导管内癌且3例伴微浸润）。乳腺癌中分子亚型分类Luminal A型693例（60.63%）；Luminal B型127例（11.11%）；Her-2过表达型130例（11.37%）；正常乳腺样（normal breast -like）型172例（15.05%）；Basal-like型21例（1.84%）。结论：对乳腺定期检查，提高早期诊断率，以期早期发现及时治疗。
基金supported by the PUMC Youth Fundthe Fundamental Research Funds for the Central Universities (3332015060)
文摘Objective： The novel fully automated immunohistochemistry （IHC） assay-Ventana anaplastic lymphoma kinase （ALK）-DSF3 for screening ALK rearrangements has been approved by China＇s Food and Drug Administration in 2013, our previous study disclosed a highly specificity and sensitivity nearly 100%, and its efficacy needs to be evaluated in a large cohort of primary lung adenocarcinoma patients, and to compare clinicopathological features with ALK （＋） and ALK （-） lung adenocarcinoma.Methods： A total of 1,504 consecutive surgical lung adenocareinoma eases of Chinese Han population were collected and re-diagnosed according to the 2011 multidisciplinary classification of lung adenocarcinoma. Fully automated Ventana ALK-D5F3 IHC staining with a binary scoring was adopted to evaluate staining and correlated with dinieopathologieal characters, including age, sex, differentiation degree, histological subtype, lymph node metastasis, and clinical staging. ALK （＋） patients were followed-up, and targeted therapy of ALKinhibitors was adopted and observed in patients with stage IV according to the NCCN guideline.Results： ALK positive adenocarcinomas were identified in 6.6% of the surgically resected 1,504 NSCLCs, and significantly younger than the negative group （P〈0.05）.Mucinous adenocarcinoma （28.2%） was determined to be predominant in ALK （＋） cases, followed by the solid type （11.7%）, specific type （6.8%）, papillary type （5.6%）, acinar type （5.5%）, and lepidic type （3.1%）, and the differences were statistically significant （χ2=42.01 1, P〈0.05）. ALK （＋） adenocarcinoma with lymph node metastasis （10.8%） were significantly higher than that without lymph node metastasis （4.5%） （g2=19.809, P〈0.05）; and ALK （＋） in phase Ⅳ （20%） was significantly higher than phaseⅢ （12.9%）, phase Ⅱ （4.2%）, phase Ⅰ （4.5%）, and phase 0 （0） （g2=36.068, P〈0.05）. Multivariate logistic regression disclosed that patient age, A
文摘目的:分析食管原发性恶性黑色素瘤(primary malignant melanoma of the esophagus,PMME)的病理诊断特点、鉴别诊断、生物学行为及预后。方法:收集安阳市肿瘤医院5例PMME,对其进行光镜观察、免疫组织化学染色、临床病理分析并进行随访。结果:PMME多见于中老年人,常发生于食管中段,大体呈蕈伞型。光镜下瘤细胞异型性明显,呈梭形、卵圆形或不规则形,以梭形细胞为主。肿瘤细胞胞核体积大,核仁清晰、染色质丰富。瘤细胞形态多样且无色素易误诊为分化差的鳞状细胞癌、肉瘤样癌、淋巴瘤等。免疫组织化学结果肿瘤细胞显示vimentin,S-100,HMB-45和Melan-A阳性;不表达神经特异性烯醇化酶(neuron-specific enolase,NSE)、白细胞共同抗原(leukocyte common antigen,LCA)、细胞角蛋白(cytokeratin,CK)、嗜铬素蛋白-A(chromogranin proteins A,Cg A)和突触素(syn)。随瘤细胞浸润不同组织层次可伴有淋巴结转移。结论:PMME是一种罕见肿瘤,恶性度高、预后差。由于其症状与食管其它肿瘤相似,在活检或手术前很难确诊,对发生于中老年人的食管中段蕈伞型肿物应考虑原发性恶性黑色素瘤的可能。临床病理分析肿瘤大小、浸润层次、淋巴结转移及治疗方式可能影响预后。
