Banana bunchy top virus （BBTV） （Nanoviridae： Babuvirus） is transmitted by aphids of the genus Pentalonia in a circulative manner. The cellular mechanisms by which BBTV translocates from the anterior midgut to the...Banana bunchy top virus （BBTV） （Nanoviridae： Babuvirus） is transmitted by aphids of the genus Pentalonia in a circulative manner. The cellular mechanisms by which BBTV translocates from the anterior midgut to the salivary gland epithelial tissues are not understood. Here, we used multiple fluorescent markers to study the distribution and the cellular localization of early and late endosomes, macropinosomes, lysosomes, microtubules, actin filaments, and lipid raft subdomains in the gut and principal salivary glands ofPentalonia nigronervosa. We applied colabeling assays, to colocalize BBTV viral particles with these cellular compartments and structures. Our results suggest that multiple potential cellular processes, including clathrin- and caveolae-mediated endocytosis and lipid rafts, may not be involved in BBTV internalization.展开更多
Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent,circulative manner by homopteran insects.Using fluorescence 2-D difference gel electrophoresis to ...Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent,circulative manner by homopteran insects.Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of F2 genotypes of Schizaphis graminum segregating for virus transmission ability,we recently discovered a panel of protein biomarkers that predict vector competency.Here we used aphid and whitefly nucleotide and expressed sequence tag database mining to test whether these biomarkers are conserved in other homopteran insects.S.graminum gene homologs that shared a high degree of predicted amino acid identity were discovered in two other aphid species and in the whitefly Bemisia tabaci.Selected reaction monitoring mass spectrometry was used to validate the expression of these biomarkers proteins in multiple aphid vector species.The conservation of these proteins in multiple insect taxa that transmit plant viruses along the circulative transmission pathway creates the opportunity to use these biomarkers to rapidly identify insect populations that are the most efficient vectors and allow them to be targeted for control prior to the spread of virus within a crop.展开更多
针对指挥控制组织结构设计中任务—平台关系的设计问题,提出了一种使命完成时间限制条件下的问题的设计模型及其求解算法。分析了使命完成时间限制条件下任务—平台关系设计(task-platform relation de-sign under mission completion t...针对指挥控制组织结构设计中任务—平台关系的设计问题,提出了一种使命完成时间限制条件下的问题的设计模型及其求解算法。分析了使命完成时间限制条件下任务—平台关系设计(task-platform relation de-sign under mission completion time constraint,TPRDTC)问题的约束条件,建立了以使命执行质量的值最大为目标的问题数学模型。设计了用于求解该模型的循环多动态列表规划(multi-dimensional dynamic list scheduling,MDLS)算法,给出了该算法的详细步骤和流程。最后通过一个联合作战的战役案例,分析并验证了循环MDLS算法对求解TPRDTC问题的有效性和适用性。展开更多
文摘Banana bunchy top virus （BBTV） （Nanoviridae： Babuvirus） is transmitted by aphids of the genus Pentalonia in a circulative manner. The cellular mechanisms by which BBTV translocates from the anterior midgut to the salivary gland epithelial tissues are not understood. Here, we used multiple fluorescent markers to study the distribution and the cellular localization of early and late endosomes, macropinosomes, lysosomes, microtubules, actin filaments, and lipid raft subdomains in the gut and principal salivary glands ofPentalonia nigronervosa. We applied colabeling assays, to colocalize BBTV viral particles with these cellular compartments and structures. Our results suggest that multiple potential cellular processes, including clathrin- and caveolae-mediated endocytosis and lipid rafts, may not be involved in BBTV internalization.
基金supported by NSF BREAD IOS:1109989,USDA-NRI 2007-04567,NSFDBI-0606596USDA-ARS 764 CRIS projects1907-101-16,1907-21000-024/25-00DNIH/NCRR funded Yeast Resource Center P41RR01182
文摘Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent,circulative manner by homopteran insects.Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of F2 genotypes of Schizaphis graminum segregating for virus transmission ability,we recently discovered a panel of protein biomarkers that predict vector competency.Here we used aphid and whitefly nucleotide and expressed sequence tag database mining to test whether these biomarkers are conserved in other homopteran insects.S.graminum gene homologs that shared a high degree of predicted amino acid identity were discovered in two other aphid species and in the whitefly Bemisia tabaci.Selected reaction monitoring mass spectrometry was used to validate the expression of these biomarkers proteins in multiple aphid vector species.The conservation of these proteins in multiple insect taxa that transmit plant viruses along the circulative transmission pathway creates the opportunity to use these biomarkers to rapidly identify insect populations that are the most efficient vectors and allow them to be targeted for control prior to the spread of virus within a crop.
文摘针对指挥控制组织结构设计中任务—平台关系的设计问题,提出了一种使命完成时间限制条件下的问题的设计模型及其求解算法。分析了使命完成时间限制条件下任务—平台关系设计(task-platform relation de-sign under mission completion time constraint,TPRDTC)问题的约束条件,建立了以使命执行质量的值最大为目标的问题数学模型。设计了用于求解该模型的循环多动态列表规划(multi-dimensional dynamic list scheduling,MDLS)算法,给出了该算法的详细步骤和流程。最后通过一个联合作战的战役案例,分析并验证了循环MDLS算法对求解TPRDTC问题的有效性和适用性。
