AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time...AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time PCR method,which is capable of differentiation of HBV viral genomic DNA and cccDNA,was used to quantify the total HBV cccDNA.The total HBV viral DNA was quantitated by real-time PCR using a HBV diagnostic kit (PG Biotech,LTD,Shenzhen,China) described previously. RESULTS:For the first time,we measured the level of HBV DNA and cccDNA isolated from ten HBV patients' liver biopsies and sera.In the liver biopsies,cccDNA was detected from all the biopsy samples.The copy number of cccDNA ranged from from 0.03 to 173.1 per cell,the copy number of total HBV DNA ranged from 0.08 to 3 717 per cell.The ratio of total HBV DNA to cccDNA ranged from 1 to 3 406.In the sera, cccDNA was only detected from six samples whereas HBV viral DNA was detected from all ten samples.The ratio of cccDNA to total HBV DNA ranged from 0 to 1.77%.To further investigate the reason why cccDNA could only be detected in some patients' sera,we performed longitudinal studies.The cccDNA was detected from the patients' sera with HBV reactivation but not from the patients' sera without HBV reactivation.The level of cccDNA in the sera was correlated with ALT and viral load in the HBV reactivation patients. CONCLUSION:HBV cccDNA is actively transcribed and replicated in some patients' hepatoo/tes,which is reflected by a high ratio of HBV total DNA vs cccDNA.Detection of cccDNA in the liver biopsy will provide an end-point for the anti-HBV therapy.The occurrence of cccDNA in the sera is an early signal of liver damage,which may be another important clinical parameter.展开更多
The rotating circular plate is widely used in mechanical engineering, meanwhile the plates are often in the electromagnetic field in modern industry with complex loads. In order to study the resonance of a rotating ci...The rotating circular plate is widely used in mechanical engineering, meanwhile the plates are often in the electromagnetic field in modern industry with complex loads. In order to study the resonance of a rotating circular plate under static loads in magnetic field, the nonlinear vibration equation about the spinning circular plate is derived according to Hamilton principle. The algebraic expression of the initial deflection and the magneto elastic forced disturbance differential equation are obtained through the application of Galerkin integral method. By mean of modified Multiple scale method, the strongly nonlinear amplitude-frequency response equation in steady state is established. The amplitude frequency characteristic curve and the relationship curve of amplitude changing with the static loads and the excitation force of the plate are obtained according to the numerical calculation. The influence of magnetic induction intensity, the speed of rotation and the static loads on the amplitude and the nonlinear characteristics of the spinning plate are analyzed. The proposed research provides the theory reference for the research of nonlinear resonance of rotating plates in engineering.展开更多
Using a circular incremental step load and unload method, a set of rheological experiments were performed to study the viscoelasto-plastic properties of amphibolite in Jinchuan No.2 diggings under different environmen...Using a circular incremental step load and unload method, a set of rheological experiments were performed to study the viscoelasto-plastic properties of amphibolite in Jinchuan No.2 diggings under different environments. Based on the scientific analysis on rheological experimental data, the viscoelasto-plastic properties of amphibolite under different environments were studied. The results show that the instantaneous elastic modulus and viscoplastic properties of amphibolite are affected little, the viscoelastic properties of amphibolite are significantly affected. Based on the experimental results and characteristics of recovered rock specimen, a generalized Kelvin model was chosen to simulate rheological properties of amphibolites and key parameters were obtained. It is found that the creep deformation modulus E1 is significantly influenced by the water, while the instant elastic modulus E2 is not significantly affected. The discreteness of the viscosity coefficient η is large, the influence of water on η is not clear and needs to be further studied.展开更多
In this paper, a novel efficient energy absorber with free inversion of a metal foam-filled circular tube(MFFCT) is designed, and the axial compressive behavior of the MFFCT under free inversion is studied analyticall...In this paper, a novel efficient energy absorber with free inversion of a metal foam-filled circular tube(MFFCT) is designed, and the axial compressive behavior of the MFFCT under free inversion is studied analytically and numerically. The theoretical analysis reveals that the energy is mainly dissipated through the radial bending of the metal circular tube, the circumferential expansion of the metal circular tube, and the metal filled-foam compression. The principle of energy conservation is used to derive the theoretical formula for the minimum compressive force of the MFFCT over free inversion under axial loading. Furthermore, the free inversion deformation characteristics of the MFFCT are analyzed numerically. The theoretical steady values are found to be in good agreement with the results of the finite element(FE) analysis. The effects of the average diameter of the metal tube, the wall thickness of the metal tube, and the filled-foam strength on the free inversion deformation of the MFFCT are considered. It is observed that in the steady deformation stage, the load-carrying and energy-absorbing capacities of the MFFCT increase with the increase in the average diameter of the metal tube, the wall thickness of the metal tube, or the filled-foam strength. The specific energy absorption(SEA) of free inversion of the MFFCT is significantly higher than that of the metal tube alone.展开更多
With the terms of the exact series solution taken as trial functions, the method of point collocation was used to calculate the large deflection of a circular plate. The axisymmetrical bending formulae were developed ...With the terms of the exact series solution taken as trial functions, the method of point collocation was used to calculate the large deflection of a circular plate. The axisymmetrical bending formulae were developed for the calculation of a circular plate subjected to polynomial distributed loads, a concentrated load at the center, uniform radial forces and moments along the edge or their combinations. The support may be elastic. The buckling load was calculated. Under action of uniformly distributed load, central load or their compound load, solutions were compared with those obtained by other methods. Buckling beyond critical thrust was compared with that calculated by the power series method. The method presented in this paper has advantages of wide convergent range, high precision and short computing time. Moreover, the computing time is nearly independent of the complexity of the loads.展开更多
基金SuppoSed by CRCG grant from the University of Hong KongCERG grant from University Grant Council of Hong Kong Research Fund from Science and Technology Commission of Shanghai,China
文摘AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time PCR method,which is capable of differentiation of HBV viral genomic DNA and cccDNA,was used to quantify the total HBV cccDNA.The total HBV viral DNA was quantitated by real-time PCR using a HBV diagnostic kit (PG Biotech,LTD,Shenzhen,China) described previously. RESULTS:For the first time,we measured the level of HBV DNA and cccDNA isolated from ten HBV patients' liver biopsies and sera.In the liver biopsies,cccDNA was detected from all the biopsy samples.The copy number of cccDNA ranged from from 0.03 to 173.1 per cell,the copy number of total HBV DNA ranged from 0.08 to 3 717 per cell.The ratio of total HBV DNA to cccDNA ranged from 1 to 3 406.In the sera, cccDNA was only detected from six samples whereas HBV viral DNA was detected from all ten samples.The ratio of cccDNA to total HBV DNA ranged from 0 to 1.77%.To further investigate the reason why cccDNA could only be detected in some patients' sera,we performed longitudinal studies.The cccDNA was detected from the patients' sera with HBV reactivation but not from the patients' sera without HBV reactivation.The level of cccDNA in the sera was correlated with ALT and viral load in the HBV reactivation patients. CONCLUSION:HBV cccDNA is actively transcribed and replicated in some patients' hepatoo/tes,which is reflected by a high ratio of HBV total DNA vs cccDNA.Detection of cccDNA in the liver biopsy will provide an end-point for the anti-HBV therapy.The occurrence of cccDNA in the sera is an early signal of liver damage,which may be another important clinical parameter.
基金Supported by National Natural Science Foundation of China(Grant No11472239)Hebei Provincial Natural Science Foundation of China(Grant No.A2015203023)Key Project of Science and Technology Research of Higher Education of Hebei Province of China(Grant No.ZD20131055)
文摘The rotating circular plate is widely used in mechanical engineering, meanwhile the plates are often in the electromagnetic field in modern industry with complex loads. In order to study the resonance of a rotating circular plate under static loads in magnetic field, the nonlinear vibration equation about the spinning circular plate is derived according to Hamilton principle. The algebraic expression of the initial deflection and the magneto elastic forced disturbance differential equation are obtained through the application of Galerkin integral method. By mean of modified Multiple scale method, the strongly nonlinear amplitude-frequency response equation in steady state is established. The amplitude frequency characteristic curve and the relationship curve of amplitude changing with the static loads and the excitation force of the plate are obtained according to the numerical calculation. The influence of magnetic induction intensity, the speed of rotation and the static loads on the amplitude and the nonlinear characteristics of the spinning plate are analyzed. The proposed research provides the theory reference for the research of nonlinear resonance of rotating plates in engineering.
基金Project (10972238) supported by the National Natural Science Foundation of ChinaProject (2009bsxt061) supported by the Thesis Innovation Funds for the Central South University, ChinaProject (2009) supported by Hunan Administration of Work Safety Fund, China
文摘Using a circular incremental step load and unload method, a set of rheological experiments were performed to study the viscoelasto-plastic properties of amphibolite in Jinchuan No.2 diggings under different environments. Based on the scientific analysis on rheological experimental data, the viscoelasto-plastic properties of amphibolite under different environments were studied. The results show that the instantaneous elastic modulus and viscoplastic properties of amphibolite are affected little, the viscoelastic properties of amphibolite are significantly affected. Based on the experimental results and characteristics of recovered rock specimen, a generalized Kelvin model was chosen to simulate rheological properties of amphibolites and key parameters were obtained. It is found that the creep deformation modulus E1 is significantly influenced by the water, while the instant elastic modulus E2 is not significantly affected. The discreteness of the viscosity coefficient η is large, the influence of water on η is not clear and needs to be further studied.
基金Project supported by the National Natural Science Foundation of China (Nos. 12272290 and11872291)the State Key Laboratory of Automotive Safety and Energy of China (No. KFY2202)。
文摘In this paper, a novel efficient energy absorber with free inversion of a metal foam-filled circular tube(MFFCT) is designed, and the axial compressive behavior of the MFFCT under free inversion is studied analytically and numerically. The theoretical analysis reveals that the energy is mainly dissipated through the radial bending of the metal circular tube, the circumferential expansion of the metal circular tube, and the metal filled-foam compression. The principle of energy conservation is used to derive the theoretical formula for the minimum compressive force of the MFFCT over free inversion under axial loading. Furthermore, the free inversion deformation characteristics of the MFFCT are analyzed numerically. The theoretical steady values are found to be in good agreement with the results of the finite element(FE) analysis. The effects of the average diameter of the metal tube, the wall thickness of the metal tube, and the filled-foam strength on the free inversion deformation of the MFFCT are considered. It is observed that in the steady deformation stage, the load-carrying and energy-absorbing capacities of the MFFCT increase with the increase in the average diameter of the metal tube, the wall thickness of the metal tube, or the filled-foam strength. The specific energy absorption(SEA) of free inversion of the MFFCT is significantly higher than that of the metal tube alone.
文摘With the terms of the exact series solution taken as trial functions, the method of point collocation was used to calculate the large deflection of a circular plate. The axisymmetrical bending formulae were developed for the calculation of a circular plate subjected to polynomial distributed loads, a concentrated load at the center, uniform radial forces and moments along the edge or their combinations. The support may be elastic. The buckling load was calculated. Under action of uniformly distributed load, central load or their compound load, solutions were compared with those obtained by other methods. Buckling beyond critical thrust was compared with that calculated by the power series method. The method presented in this paper has advantages of wide convergent range, high precision and short computing time. Moreover, the computing time is nearly independent of the complexity of the loads.
