AIM To study the interaction between ciprofloxacin and BSA in physiological condition by fluorescence spectroscopy. METHODS The affection of drug to the protein conformation was investigated. The binding constant betw...AIM To study the interaction between ciprofloxacin and BSA in physiological condition by fluorescence spectroscopy. METHODS The affection of drug to the protein conformation was investigated. The binding constant between drug and BSA from a double reciprocal Lineweaver Burk plot was determined and the main sort of binding force was found according to the thermodynamic parameters. RESULTS The binding constants between BSA and ciprofloxacin at 26℃and 45℃ are about 10 4. At 26℃, the thermodynamic parameters of reaction between BSA and ciprofloxacin are Δ H = -49 13 kJ·mol -1 , Δ G = -26 45 kJ·mol -1 , Δ S = -75 kJ·mol -1 . The maximum wavelength of the synchronous fluorescence spectra of BSA moved from 279 nm to 289nm with the incresing of the amount of ciprofloxacin. CONCLUSION There exists fluorescence energy transfer between BSA and ciprofloxacin. The main sort of binding force between BSA and ciprofloxacin is Van der Waals′ interaction. Ciprofloxacin can be deposited and be transported by serum protein in vivo . Ciprofloxacin affects the protein conformation.展开更多
采用碱活化过一硫酸盐(peroxymonosulfate,PMS)对环丙沙星(ciprofloxacin,CIP)的去除进行了系统地研究.结果表明,碱活化PMS体系能够高效地去除CIP.通过自由基捕获实验确定了单线态氧(~1O_2)和超氧自由基(O_2^-·)是反应体系中的主...采用碱活化过一硫酸盐(peroxymonosulfate,PMS)对环丙沙星(ciprofloxacin,CIP)的去除进行了系统地研究.结果表明,碱活化PMS体系能够高效地去除CIP.通过自由基捕获实验确定了单线态氧(~1O_2)和超氧自由基(O_2^-·)是反应体系中的主要活性物种.NaOH浓度、PMS浓度、反应温度和共存阴离子等对CIP在碱活化PMS体系中的去除均有一定影响.随着NaOH和PMS浓度的增加,CIP的降解均呈现出先增加后降低的趋势.提高反应温度能够加大CIP的反应速率,经过阿伦尼乌斯方程拟合得到的反应活化能为5.09 k J·mol^(-1).不同的阴离子对CIP的去除呈现不同的影响:Cl^-、SO_4^(2-)和NO_3^-对CIP的降解没有呈现明显的作用,H_2PO_4^(2-)能够有效地抑制环丙沙星的去除,而CO_3^(2-)极大地促进了反应进程.通过UPLC-MS/MS可检测到10种降解产物,CIP分子结构中的哌嗪环易于受到活性物种的攻击.展开更多
文摘AIM To study the interaction between ciprofloxacin and BSA in physiological condition by fluorescence spectroscopy. METHODS The affection of drug to the protein conformation was investigated. The binding constant between drug and BSA from a double reciprocal Lineweaver Burk plot was determined and the main sort of binding force was found according to the thermodynamic parameters. RESULTS The binding constants between BSA and ciprofloxacin at 26℃and 45℃ are about 10 4. At 26℃, the thermodynamic parameters of reaction between BSA and ciprofloxacin are Δ H = -49 13 kJ·mol -1 , Δ G = -26 45 kJ·mol -1 , Δ S = -75 kJ·mol -1 . The maximum wavelength of the synchronous fluorescence spectra of BSA moved from 279 nm to 289nm with the incresing of the amount of ciprofloxacin. CONCLUSION There exists fluorescence energy transfer between BSA and ciprofloxacin. The main sort of binding force between BSA and ciprofloxacin is Van der Waals′ interaction. Ciprofloxacin can be deposited and be transported by serum protein in vivo . Ciprofloxacin affects the protein conformation.
文摘采用碱活化过一硫酸盐(peroxymonosulfate,PMS)对环丙沙星(ciprofloxacin,CIP)的去除进行了系统地研究.结果表明,碱活化PMS体系能够高效地去除CIP.通过自由基捕获实验确定了单线态氧(~1O_2)和超氧自由基(O_2^-·)是反应体系中的主要活性物种.NaOH浓度、PMS浓度、反应温度和共存阴离子等对CIP在碱活化PMS体系中的去除均有一定影响.随着NaOH和PMS浓度的增加,CIP的降解均呈现出先增加后降低的趋势.提高反应温度能够加大CIP的反应速率,经过阿伦尼乌斯方程拟合得到的反应活化能为5.09 k J·mol^(-1).不同的阴离子对CIP的去除呈现不同的影响:Cl^-、SO_4^(2-)和NO_3^-对CIP的降解没有呈现明显的作用,H_2PO_4^(2-)能够有效地抑制环丙沙星的去除,而CO_3^(2-)极大地促进了反应进程.通过UPLC-MS/MS可检测到10种降解产物,CIP分子结构中的哌嗪环易于受到活性物种的攻击.
